Plumbagin ameliorates bile duct ligation-induced cholestatic liver injury in rats

Pan, Pin-Ho; Wang, Yayu; Lin, Shih‐Yi; Liao, Su‐Lan; Chen, Yu‐Fang; Huang, Wei-Chi; Chen, Chun‐Jung; Chen, Wenying

doi:10.1016/j.biopha.2022.113133

Cited by 14 publications

(7 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was further confirmed histologically by the detection of remarkable inflammatory changes with marked fibrosis, which may be brought on by HMGB1/RAGE overexpression. Similar findings were obtained by Pan et al 46 . NF-κB is a pleiotropic transcription factor that plays a fundamental role in fibrogenesis by inducing the expression of numerous pro-inflammatory 47 , as well as pro-fibrogenic cytokines 48 .…”

Section: Discussionsupporting

confidence: 92%

Diacerein ameliorates cholestasis-induced liver fibrosis in rat via modulating HMGB1/RAGE/NF-κB/JNK pathway and endoplasmic reticulum stress

Abdelfattah

Mahmoud

El-Wafaey

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Diacerein is an interleukin (IL)-1β inhibitor approved for osteoarthritis. This study aimed to investigate the potential anti-fibrotic effect of diacerein against bile duct ligation (BDL)-induced liver fibrosis. Forty male Wistar rats were divided into: sham-operated group, BDL group, and BDL groups treated with diacerein at 10, 30, and 50 mg/kg/day starting two days before surgery and continued for 4 weeks. Diacerein decreased the hepatic injury markers and alleviated oxidative stress triggered by BDL by reducing hepatic malondialdehyde (MDA) and increasing hepatic superoxide dismutase (SOD) levels. Diacerein mitigated BDL-induced inflammation via lowering hepatic levels and mRNA expression of high mobility group box 1 (HMGB1), nuclear factor-κB (NF-κB), and IL-1β. The hepatic gene expression of Advanced Glycation End products Receptor (RAGE) gene and immunohistochemical expression of some ER stress markers, e.g., glucose-regulated protein 78 (GRP78), inositol-requiring enzyme 1 (IRE1α), protein kinase RNA-like endoplasmic reticulum kinase (PERK), CCAAT/enhancer-binding protein homologous protein (CHOP), and phosphorylated c-Jun N-terminal kinase protein contents were lowered by diacerein. Furthermore, diacerein suppressed the hepatic levels of fibrogenic mediators, e.g., Transforming growth factor β1 (TGF˗β1), α- smooth muscle actin (α-SMA), collagen 1, and hydroxyproline, as well as the apoptotic caspase 3 and BAX immunostaining in BDL rats. The histopathological abnormalities induced by BDL significantly improved. Our study demonstrated that diacerein exhibited an antifibrotic effect by inhibiting HMGB1/RAGE/NF-κB/JNK pathway, and ER stress. Better protection was observed with increasing the dose.

show abstract

Section: Discussionsupporting

confidence: 92%

Diacerein ameliorates cholestasis-induced liver fibrosis in rat via modulating HMGB1/RAGE/NF-κB/JNK pathway and endoplasmic reticulum stress

Abdelfattah

Mahmoud

El-Wafaey

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Previous research on Pseudomonas aeruginosa keratitis has validated the potential of targeting HMGB1 as an anti-inflammatory approach (Ekanayaka et al, 2018 ; Hazlett et al, 2021 ). Meanwhile, PL has been shown to effectively inhibit HMGB1 in different disease models, including cholestatic liver injury, hepatic ischemic re-injury, and sepsis (Zhang Z. et al, 2016 ; Zaki et al, 2018 ; Pan et al, 2022 ). This research prompted us to conduct related experiments on corneal and peritoneal macrophages in mice.…”

Section: Discussionmentioning

confidence: 99%

“…Plumbagin (5-hydroxy-2-methyl-1, 4-naphthoquinone, PL) is a potent naphthoquinone compound from the roots of traditional medicinal plants. It has a variety of pharmacological properties (Jaradat et al, 2017 ) and has been proven to have anti-inflammatory, antimicrobial activity, anti-oxidative stress and anti-cancer effects (Pan et al, 2022 ; Catalani et al, 2023 ; Lin et al, 2023 ; Xiong et al, 2024 ). PL has been utilized in various inflammatory models, such as the lipopolysaccharide (LPS)-induced glial cell inflammation model and rheumatoid arthritis, to downregulate the expression of inflammatory factors, including TNF-α, IL-6, and IL-1β (Gupta et al, 2018 ; Shu et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Plumbagin inhibits fungal growth, HMGB1/LOX-1 pathway and inflammatory factors in A. fumigatus keratitis

Cong,

Gu,

Lin

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

To investigate the anti-inflammatory and antifungal effects of plumbagin (PL) in Aspergillus fumigatus (A. fumigatus) keratitis, the minimum inhibitory concentration (MIC), time-killing curve, spore adhesion, crystal violet staining, calcium fluoride white staining, and Propidium Iodide (PI) staining were employed to assess the antifungal activity of PL in vitro against A. fumigatus. The cytotoxicity of PL was assessed using the Cell Counting Kit-8 (CCK8). The impact of PL on the expression of HMGB1, LOX-1, TNF-α, IL-1β, IL-6, IL-10 and ROS in A. fumigatus keratitis was investigated using RT-PCR, ELISA, Western blot, and Reactive oxygen species (ROS) assay. The therapeutic efficacy of PL against A. fumigatus keratitis was assessed through clinical scoring, plate counting, Immunofluorescence and Hematoxylin-Eosin (HE) staining. Finally, we found that PL inhibited the growth, spore adhesion, and biofilm formation of A. fumigatus and disrupted the integrity of its cell membrane and cell wall. PL decreased IL-6, TNF-α, and IL-1β levels while increasing IL-10 expression in fungi-infected mice corneas and peritoneal macrophages. Additionally, PL significantly attenuated the HMGB1/LOX-1 pathway while reversing the promoting effect of Boxb (an HMGB1 agonist) on HMGB1/LOX-1. Moreover, PL decreased the level of ROS. In vivo, clinical scores, neutrophil recruitment, and fungal burden were all significantly reduced in infected corneas treated with PL. In summary, the inflammatory process can be inhibited by PL through the regulation of the HMGB-1/LOX-1 pathway. Simultaneously, PL can exert antifungal effects by limiting fungal spore adhesion and biofilm formation, as well as causing destruction of cell membranes and walls.

show abstract

“…This was related to an increase in hepatocyte proliferation and reduced apoptosis levels [ 30 ]. Autophagy as a protective mechanism induced by PB was reported by Pan and colleagues in rat hepatic tissue of bile duct ligation (BDL)-induced cholestatic liver injury [ 31 ].…”

Section: The Cytoprotective Activity Of Pbmentioning

confidence: 99%

“…Similar findings were obtained in thioacetamide-induced hepatic fibrosis, where PB inhibited the Nf-kB and Akt pathways in the hepatic stellate cells, blocking the collagen deposition [ 30 ]. In addition to the α-SMA protein expression, PB downregulated the transforming growth factor (TGF)1-β protein expression in myofibroblasts and hepatic stellate cells [ 31 ].…”

Section: The Anti-inflammatory Activity Of Pbmentioning

confidence: 99%

Plumbagin, a Natural Compound with Several Biological Effects and Anti-Inflammatory Properties

Petrocelli

Marrazzo

Bonsi

et al. 2023

Life

View full text Add to dashboard Cite

Phytochemicals from various medicinal plants are well known for their antioxidant properties and anti-cancer effects. Many of these bioactive compounds or natural products have demonstrated effects against inflammation, while some showed a role that is only approximately described as anti-inflammatory. In particular, naphthoquinones are naturally-occurring compounds with different pharmacological activities and allow easy scaffold modification for drug design approaches. Among this class of compounds, Plumbagin, a plant-derived product, has shown interesting counteracting effects in many inflammation models. However, scientific knowledge about the beneficial effect of Plumbagin should be comprehensively reported before candidating this natural molecule into a future drug against specific human diseases. In this review, the most relevant mechanisms in which Plumbagin plays a role in the process of inflammation were summarized. Other relevant bioactive effects were reviewed to provide a complete and compact scenario of Plumbagin’s potential therapeutic significance.

show abstract

Plumbagin ameliorates bile duct ligation-induced cholestatic liver injury in rats

Cited by 14 publications

References 69 publications

Diacerein ameliorates cholestasis-induced liver fibrosis in rat via modulating HMGB1/RAGE/NF-κB/JNK pathway and endoplasmic reticulum stress

Diacerein ameliorates cholestasis-induced liver fibrosis in rat via modulating HMGB1/RAGE/NF-κB/JNK pathway and endoplasmic reticulum stress

Plumbagin inhibits fungal growth, HMGB1/LOX-1 pathway and inflammatory factors in A. fumigatus keratitis

Plumbagin, a Natural Compound with Several Biological Effects and Anti-Inflammatory Properties

Contact Info

Product

Resources

About