Plumbagin production by root cultures of Plumbago rosea

Panichayupakaranant, Pharkphoom; Tewtrakul, Supinya

doi:10.2225/vol5-issue3-fulltext-4

Cited by 29 publications

(27 citation statements)

References 8 publications

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“…Effect of cytokinins in combination with NAA on rooting depends on kind and concentration of cytokinin. Panichayupakaranant and Tewtrakul (2002) reported that the combination of NAA and Kn was found to be best for root growth in P. rosea. However, in the present study, the combination of NAA and Kn was not suitable for increasing the rooting percentage.…”

Section: Resultsmentioning

confidence: 99%

Establishment of In vitro Adventitious Root Cultures and Analysis of Flavonoids in Rumex crispus

Mahdieh

Noori

Hoseinkhani

2015

Plant Tissue Cult. & Biotech.

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Adventitious root culture of leaf explants of R. crispus was established using different MS supplemented with different concentrations of auxins and a combination of NAA and Kn for growth and flavonoids production. Among the different auxins, NAA was more effective than IAA to induce adventitious roots. Adventitious roots grown on MS containing 5 μM NAA and 0.5 μM Kn showed the highest root growth, as well as the highest amount of total flavonoids (= 6) as compared with roots grown in other media. Chromatographic purification of the root extract showed that flavonoid composition also was influenced by hormone combinations in the culture media. The addition of Kn to the medium reduced or suppressed myricetin (M) and naringenin (N) production. Quercetin (Q) was not found in media containing Kn alone similar to the control medium. Isorhamnetin (I), kaempferol (K) and rutin (R) were produced in the roots on media supplemented with all hormone combinations, but were absent in 0.1 μM Kn supplemented media similar to the control roots.

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Section: Resultsmentioning

confidence: 99%

Establishment of In vitro Adventitious Root Cultures and Analysis of Flavonoids in Rumex crispus

Mahdieh

Noori

Hoseinkhani

2015

Plant Tissue Cult. & Biotech.

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“…Previous studies (Paiva unplublished data) showed that, in P. scandens, this naphthoquinone is found predominantly on the roots, with low concentrations in aerial parts. In other species of the genus Plumbago, such as P. rosea, the literature data also point to the accumulation of plumbagin in roots (Panichayupakaranant and Tewtrakul 2002). A study carried out by Verma et al 2002, revealed the potentialities of the hairy root cultures of P. zeylanica for the production of plumbagin.…”

Section: Resultsmentioning

confidence: 99%

Chemical composition fluctuations in roots of Plumbago scandens L. in relation to floral development

Paiva

Lima

Figueiredo

et al. 2011

An. Acad. Bras. Ciênc.

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Plumbago scandens L. is a Brazilian tropical/subtropical species that occurs along the coast. Chemically it is mainly represented by naphthoquinones, flavonoids, terpenoids and steroids. The aim of the present work is to study quantitative changes in the root metabolic production of Plumbago scandens during different physiologic developmental stages relative to floration. The results indicated the presence of four substances in the extracts: plumbagin, epi-isoshinanolone, palmitic acid and sitosterol, independent on developmental stage. The naphthoquinone plumbagin has always showed to be the major component of all extracts. Naphthoquinones exhibited their highest content during floration, while the content of the two others components decreased during this stage, revealing an inverse profile. The chemical composition changed depending on the plant requirements.

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“…Moreover, the low germination of seeds and death of young plantlets under natural environment makes conventional propagation of the plant challenging and inadequate to meet the growing demand of the pharmaceutical companies. Various in vitro protocols have been used in an effort to boost plumbagin production in Plumbago species including the induction of adventitious roots in P. indica (Panichayupakaranant and Tewtrakul 2002) and P. zeylanica (Sivanesan and Jeong 2009). …”

Section: Introductionmentioning

confidence: 99%

Genetic Transformation of Plumbago zeylanica with Agrobacterium rhizogenes Strain LBA 9402 and Characterization of Transformed Root Lines

Basu

Joshi

Jha

2015

Plant Tissue Cult. & Biotech.

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High frequency transformation (73.80 ± 2.24%) has been obtained in Plumbago zeylanica using nodes and internodes of axenic whole plants infected with Agrobacterium rhizogenes strain LBA 9402. The root lines could be distinguished morphologically into two types : Root lines of morphotypes I and II. While morphotype I showed profuse branching with short (< 1 cm), highly dense hairy laterals, the roots of morphotype II roots were characterized also by profuse branching with long hairy laterals (> 3 -4 cm). Only four of the ten root lines showed integration of four rol genes (rolA, rolB, rolC and rolD) of TL-DNA. None of the root lines showed presence of any of the five genes of TR-DNA. It is noteworthy that the root morphotypes (I and II) showed a clear distinction in the nature of integration and expression of rol genes. The transformed root lines varied significantly (p ≤ 0.05) with respect to DW (GI DW basis, 2.19 ± 0.24 -5.31 ± 0.6) after 4 weeks of culture on solid modified MS; and plumbagin contents in root lines (4.81 ± 0.16 -6.69 ± 0.34 mg/g DW) were higher than that reported earlier. Transformed root lines of P. zeylanica maintained in vitro on phytohormone devoid medium for over 2 years can be used for scale up studies for the production of plumbagin in bioreactors.

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Plumbagin production by root cultures of Plumbago rosea

Abstract: Root cultures of Plumbago rosea

Cited by 29 publications

References 8 publications

Establishment of In vitro Adventitious Root Cultures and Analysis of Flavonoids in Rumex crispus

Establishment of In vitro Adventitious Root Cultures and Analysis of Flavonoids in Rumex crispus

Chemical composition fluctuations in roots of Plumbago scandens L. in relation to floral development

Genetic Transformation of Plumbago zeylanica with Agrobacterium rhizogenes Strain LBA 9402 and Characterization of Transformed Root Lines

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