Pluripotent Hair Follicle Neural Crest Stem-Cell-Derived Neurons and Schwann Cells Functionally Repair Sciatic Nerves in Rats

Lin, Haiyan; Liu, Fang; Zhang, Chuansen; Zhang, Zhiying; Guo, Jiasong; (任丛莉), Ren Cong-li; Kong, Zhengdong

doi:10.1007/s12035-009-8082-z

Cited by 35 publications

(26 citation statements)

References 31 publications

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“…HFSCs differentiated into GFAP expressing SCs and were able to myelinate axons; gastrocnemius muscle contraction significantly greater compared with untreated nerves Lin et al [136] Rat sciatic transection (40 increase astrogliosis and potentiate the inflammatory response [8,18] . The observed immunomodulatory effects of stem cells are believed to be due to the secretion of factors such as granulocyte and macrophage colony stimulating factor (G-CSF, M-CSF), interleukin-6, 7, 8, and 11 (IL-6, IL-7, IL-8, IL-11) and TNF-α [8] .…”

Section: U Culture Medium Direct Injection At Sitementioning

confidence: 99%

“…Functional outcomes were significantly improved in those nerves receiving HFSCs. The efficacy of differentiated HFSCs seeded into acellular xenografts has also been investigated [136] . Cells were able to maintain differentiation long term and the number of regenerated axons and myelination in cell-supplemented xenografts was higher than cell-free xenografts.…”

Section: Skin Derived Precursorsmentioning

confidence: 99%

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Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Fairbairn

Meppelink

Ng-Glazier

et al. 2015

WJSC

129

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Outcomes following peripheral nerve injury remain frustratingly poor. The reasons for this are multifactorial, although maintaining a growth permissive environment in the distal nerve stump following repair is arguably the most important. The optimal environment for axonal regeneration relies on the synthesis and release of many biochemical mediators that are temporally and spatially regulated with a high level of incompletely understood complexity. The Schwann cell (SC) has emerged as a key player in this process. Prolonged periods of distal nerve stump denervation, characteristic of large gaps and proximal injuries, have been associated with a reduction in SC number and ability to support regenerating axons. Cell based therapy offers a potential therapy for the improvement of outcomes following peripheral nerve reconstruction. Stem cells have the potential to increase the number of SCs and prolong their ability to support regeneration. They may also have the ability to rescue and replenish populations of chromatolytic and apoptotic neurons following axotomy. Finally, they can be used in non-physiologic ways to preserve injured tissues such as denervated muscle while neuronal ingrowth has not yet occurred. Aside from stem cell type, careful consideration must be given to differentiation status, how stem cells are supported following transplantation and how they will be delivered to the site of injury. It is the aim of this article to review current opinions on the strategies of stem cell based therapy for the augmentation of peripheral nerve regeneration.

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Section: U Culture Medium Direct Injection At Sitementioning

confidence: 99%

Section: Skin Derived Precursorsmentioning

confidence: 99%

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Fairbairn

Meppelink

Ng-Glazier

et al. 2015

WJSC

129

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“…Bone marrow stromal cells (BMSCs), adipose-derived stromal cells (ADSCs), hair follicle stem cells, skin-derived mesenchymal stem cells, and amniotic fluid-derived mesenchymal stem cells can all be applied. 23,31,36,37,47 The different stem cells have different advantages and disadvantages. Stem cells, such as BMSCs of mesenchymal origin, can differentiate successfully into neural cells.…”

Section: ©Aans 2014mentioning

confidence: 99%

“…36 More recently applied stem cells, such as hair follicle pluripotent stem cells and skin-derived stem cells, are also easily accessible and can transdifferentiate into Schwann cell-like cells as well. 23 Although most studies investigating the use of stem cell to enhance nerve regeneration have expressed enthusiasm and have promoted further research, 25,31 researchers have not compared their own data to other studies, nor has anyone proposed an evaluation protocol that would allow a correct comparison of the results. Therefore, the purpose of this meta-analysis is to summarize the effect of different types of stem cells in animal experimental studies used to enhance regeneration after reconstructing a peripheral nerve injury.…”

Section: ©Aans 2014mentioning

confidence: 99%

The effect of stem cells in bridging peripheral nerve defects: a meta-analysis

Hundepool

Nijhuis

Mohseny

et al. 2014

JNS

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Object. For decades the gold standard for reconstructing a large peripheral nerve defect has been, and remains, the nerve autograft. Alternatives to the nerve autograft include biological conduits and vessels. Adding stem cells in the lumen of a nerve conduit has been the subject of multiple studies. The purpose of the present meta-analysis was to summarize animal experimental studies on the effect of stem cells as a luminal additive when reconstructing a peripheral nerve defect with a nerve graft.Methods. A literature search of the MEDLINE and Embase databases was performed from inception to April 2012, searching for animal experiments on peripheral nerve reconstruction models in which a nerve conduit was used with and without the support of 3 different types of stem cells. Stem cells were analyzed according to their origin: bone marrow, adipose tissue, and other origins. Included studies had consistent outcome measurements: walking track analysis, muscle mass ratio, and electrophysiology.Results. Forty-four studies were included in the final analysis. Forest plots of the 3 outcome measurements (walking track analysis, muscle mass ratio, and electrophysiology) showed positive effects of stem cells on the regeneration of peripheral nerves at different time points. Almost all comparisons showed significant differences for all 3 stem cells groups compared with a control group in which stem cells were not used.Conclusions. The present report systematically analyzed the different studies that used stem cells as a luminal additive when bridging a large peripheral nerve defect. All 3 different stem cell groups showed a beneficial effect when used in the reconstruction compared with control groups in which stem cells were not used. (http://thejns.org/doi/abs/10.3171/2014.4.JNS131260) Key WordS • peripheral nerve defect • stem cells • regeneration • meta-analysisAbbreviations used in this paper: ADSC = adipose-derived stromal cell; BMSC = bone marrow stromal cell; MM = muscle mass ratio; NCV = nerve conduction velocity SFI = Sciatic Functional Index.

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“…Few studies have analyzed the employment of undifferentiated hair follicle SCs in murine models of sciatic and tibial transection and crush injuries with improved functional outcomes in recipient nerves [66, 73–76]. In addition, differentiated hair follicle SCs placed into acellular xenografts were able to further differentiate for extended periods of time, leading to more regenerated axons with increased myelination as compared to cell-free xenografts [66, 76, 77].…”

Section: Scs and Nerve Regenerationmentioning

confidence: 99%

The Holy Grail of Orthopedic Surgery: Mesenchymal Stem Cells—Their Current Uses and Potential Applications

Berebichez-Fridman

Gómez-García

Granados-Montiel

et al. 2017

Stem Cells International

104

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Only select tissues and organs are able to spontaneously regenerate after disease or trauma, and this regenerative capacity diminishes over time. Human stem cell research explores therapeutic regenerative approaches to treat various conditions. Mesenchymal stem cells (MSCs) are derived from adult stem cells; they are multipotent and exert anti-inflammatory and immunomodulatory effects. They can differentiate into multiple cell types of the mesenchyme, for example, endothelial cells, osteoblasts, chondrocytes, fibroblasts, tenocytes, vascular smooth muscle cells, and sarcomere muscular cells. MSCs are easily obtained and can be cultivated and expanded in vitro; thus, they represent a promising and encouraging treatment approach in orthopedic surgery. Here, we review the application of MSCs to various orthopedic conditions, namely, orthopedic trauma; muscle injury; articular cartilage defects and osteoarthritis; meniscal injuries; bone disease; nerve, tendon, and ligament injuries; spinal cord injuries; intervertebral disc problems; pediatrics; and rotator cuff repair. The use of MSCs in orthopedics may transition the practice in the field from predominately surgical replacement and reconstruction to bioregeneration and prevention. However, additional research is necessary to explore the safety and effectiveness of MSC treatment in orthopedics, as well as applications in other medical specialties.

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Pluripotent Hair Follicle Neural Crest Stem-Cell-Derived Neurons and Schwann Cells Functionally Repair Sciatic Nerves in Rats

Cited by 35 publications

References 31 publications

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

The effect of stem cells in bridging peripheral nerve defects: a meta-analysis

The Holy Grail of Orthopedic Surgery: Mesenchymal Stem Cells—Their Current Uses and Potential Applications

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