Pluripotent Pericycle Cells Trigger Different Growth Outputs by Integrating Developmental Cues into Distinct Regulatory Modules

Xiao, Wei; Molina, David J.; Wunderling, Anna; Ripper, Dagmar; Vermeer, Joop E. M.; Ragni, Laura

doi:10.1016/j.cub.2020.08.053

Cited by 38 publications

(77 citation statements)

References 56 publications

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“…Also, the master regulator of vascular cambium activity KNAT1/BREVIPEDICELLUS (BP) 19 shows high expression with a maximum in April (June/April log 2 FC = -0.98). In agreement with our data and meaningfully, very recently WOX4 and BP has been demonstrated to control phellogen activity in Arabidopsis root standing as periderm-positive regulators 15 , suggesting that phellogen in herbaceous and woody plants may be regulated similarly. Other identified genes have also been observed to be highly expressed in vascular cambium: STM, WRINKLED 3 (WRI3), ANAC042 and LOB DOMAIN-CONTAINING PROTEIN 4 (LBD4), the latter also showing expression in phellogen and involved in vascular patterning 19 .…”

Section: Discussionsupporting

confidence: 93%

“…In the same line, phellogen and vascular cambium, both bifacial secondary meristems with seasonal growth, commonly upregulates a set of specific genes not present in other tree trunk tissues 14 , pointing that both meristems share molecular mechanisms for its formation and maintenance. The shared mechanisms have been evidenced very recently, since the vascular cambium master regulators WUSCHEL-RELATED HOMEOBOX 4 (WOX4) and KNAT1/BREVIPEDICELLUS (BP) were also found to control the Arabidopsis root phellogen activity 15 .…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation

Fernández-Piñán

Boher

Soler

et al. 2021

Sci Rep

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The phellogen or cork cambium stem cells that divide periclinally and outwardly specify phellem or cork. Despite the vital importance of phellem in protecting the radially-growing plant organs and wounded tissues, practically only the suberin biosynthetic process has been studied molecularly so far. Since cork oak (Quercus suber) phellogen is seasonally activated and its proliferation and specification to phellem cells is a continuous developmental process, the differentially expressed genes during the cork seasonal growth served us to identify molecular processes embracing from phellogen to mature differentiated phellem cell. At the beginning of cork growth (April), cell cycle regulation, meristem proliferation and maintenance and processes triggering cell differentiation were upregulated, showing an enrichment of phellogenic cells from which phellem cells are specified. Instead, at maximum (June) and advanced (July) cork growth, metabolic processes paralleling the phellem cell chemical composition, such as the biosynthesis of suberin, lignin, triterpenes and soluble aromatic compounds, were upregulated. Particularly in July, polysaccharides- and lignin-related secondary cell wall processes presented a maximal expression, indicating a cell wall reinforcement in the later stages of cork formation, presumably related with the initiation of latecork development. The putative function of relevant genes identified are discussed in the context of phellem ontogeny.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation

Fernández-Piñán

Boher

Soler

et al. 2021

Sci Rep

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“…In this work we show that auxin and GA, via the interaction of ARF6, ARF8 with DELLA, regulate xylem expansion, revealing a novel hormonal cross-talk during secondary growth. ARF6 and ARF8 are known to regulate flower development, adventitious root formation, and hypocotyl elongation (Nagpal et al , 2005; Goetz et al , 2006; Gutierrez et al , 2012; Liu et al , 2014) and recently, we show that ARF8 promotes periderm growth in the root (Xiao et al , 2020). Consistently with their novel role during xylem expansion, we showed that arf6 arf8 mutants display reduced xylem occupancy, diminished xylary fiber formation, retarded cambium senescence, and enhanced phloem proliferation, mimicking the expression of a undegradable version of DELLA (in Col background).…”

Section: Discussionmentioning

confidence: 78%

“…Consistently, DELLA proteins are known to physically interact with many transcription factors belonging to different families (Claeys et al , 2014; Colebrook et al , 2014; Marin-de la Rosa et al , 2014) and act as central hub connecting several signaling cascades such as auxin, cytokinins, ethylene, Jasmonate and ABA in different developmental contexts (Locascio et al , 2013). It is well established that auxin promotes different aspects of secondary growth such as cambium establishment, cambium homeostasis, xylem differentiation and periderm formation (Brackmann et al , 2018; Smetana et al , 2019; Xiao et al , 2020). In this work we show that auxin and GA, via the interaction of ARF6, ARF8 with DELLA, regulate xylem expansion, revealing a novel hormonal cross-talk during secondary growth.…”

Section: Discussionmentioning

confidence: 99%

AUXIN RESPONSE FACTOR 6(ARF6)andARF8promote Gibberellin-mediated hypocotyl xylem expansion and cambium homeostasis

Ripper

Bayer

et al. 2020

Preprint

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During secondary growth, the thickening of plant organs, wood (xylem) and bast (phloem) are continuously produced by the vascular cambium. In Arabidopsis hypocotyl and root, we can distinguish two phases of secondary growth based on cell morphology and production rate. The first phase, in which xylem and phloem are equally produced, precedes the xylem expansion phase in which xylem formation is enhanced and xylem fibers differentiate. It is known that Gibberellins (GA) trigger this developmental transition via the degradation of DELLA proteins and that the cambium master regulator BREVIPEDICELLUS/KNAT1 (BP/KNAT1) and the receptor like kinases ERECTA and ERL1 regulate this process downstream of GA. However, our understandings on the regulatory network underlying GA-mediated secondary growth, are still limited.Here, we demonstrate that DELLA-mediated xylem expansion is mainly achieved through RGA and GAI and that RGA and GAI promote cambium senescence. We further show that AUXIN RESPONSE FACTOR (ARF6) and ARF8, which physically interact with DELLAs, specifically repress phloem proliferation and induce cambium senescence during the xylem expansion phase. Moreover, the inactivation of BP in arf6 arf8 background revealed an essential role for ARF6 and ARF8 in cambium establishment and maintenance. Overall, our results shed light on a pivotal hormone cross-talk between GA and auxin in the context of plant secondary growth.

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“…Zhang et al 2019). Second, upon auxin stimulation of root pericycle cells, that exact transcription factor combination promotes establishment of a protective suberized, periderm layer rather than initiation of a proliferative stem cell niche destined to form a new lateral root (Xiao et al 2020). Transcription factors associated with growth arrest and general stress responses were also found in C2 (e.g.…”

Section: Resultsmentioning

confidence: 99%

PAMP-triggered Genetic Reprogramming Involves Widespread Alternative Transcription Initiation and an Immediate Transcription Factor Wave

Thieffry

Bornholdt

Barghetti

et al. 2021

Preprint

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Immune responses triggered by pathogen-associated molecular patterns (PAMPs) are key to pathogen defense, but drivers of the genetic reprogramming required to reach the immune state remain incompletely understood in plants. Here, we report a time-course study of the establishment of PAMP-triggered immunity (PTI) using cap analysis of gene expression (CAGE). Our results show that as much as 15% of all PAMP response genes display alternative transcription initiation. In several cases, use of alternative TSSs may be regulatory as it determines inclusion of target peptides or protein domains, or occurrence of upstream open reading frames (uORFs) in mRNA leader sequences. We also find that 60% of PAMP-response genes respond much earlier than previously thought. In particular, a previously unnoticed cluster of rapidly and transiently PAMP-induced genes is enriched in transcription factors whose functions, previously associated with biological processes as diverse as abiotic stress adaptation and stem cell activity, appear to converge on growth restriction. Furthermore, some examples of known potentiators of PTI, in one case under direct MAP kinase control, support the notion that the rapidly induced transcription factors could constitute direct links to PTI signaling pathways and drive gene expression changes underlying establishment of the immune state.

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Pluripotent Pericycle Cells Trigger Different Growth Outputs by Integrating Developmental Cues into Distinct Regulatory Modules

Cited by 38 publications

References 56 publications

Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation

Transcriptomic analysis of cork during seasonal growth highlights regulatory and developmental processes from phellogen to phellem formation

AUXIN RESPONSE FACTOR 6(ARF6)andARF8promote Gibberellin-mediated hypocotyl xylem expansion and cambium homeostasis

PAMP-triggered Genetic Reprogramming Involves Widespread Alternative Transcription Initiation and an Immediate Transcription Factor Wave

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