Pluripotent stem cell-derived neural stem cells: From basic research to applications

Otsu, Masahiro; Nakayama, Takashi; Inoue, Nozomu

doi:10.4252/wjsc.v6.i5.651

Cited by 7 publications

(2 citation statements)

References 64 publications

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“…The life span of hNSCs in vitro can be improved by optimizing culture conditions ( 3 ) or via immortalization using the myc transcription factor ( 4 ) and maintaining a stable phenotype. Stable hNSC lines, including ReNcell CX cells immortalized using c-myc and VM cells immortalized with v-myc, are widely used in investigations in a variety of neurological fields ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model

Jung

Lee

et al. 2017

Int J Mol Med

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Alternative cell models of human neural stem cells (hNSCs) have been developed and used for investigations ranging from in vitro experiments to in vivo clinical studies. However, a cell model capable of mimicking the ʻnormalʼ state of hNSCs is mandatory in order to extrapolate the results of these studies to humans. In the present study, to select a more suitable hNSC model for developing human-based experimental platforms, two representative hNSC types were compared, namely human embryonic stem cell (hESC)-derived hNSCs and ReNcell CX cells, which are well-characterized immortalized hNSC lines. The hNSCs, differentiated from hESCs via human neuroectodermal sphere (hNES) formation, recapitulated the molecular and cellular phenotypes of hNSCs, including NSC marker expression and terminal neuronal differentiation potential. Comparative analyses of the transcriptome profiles of the hESC-derived hNESs and ReNcell CX hNSCs showed that the differentiated hNESs were analogous to the ReNcell CX cells, as demonstrated by principal component analysis and hierarchical sample clustering. The hNSC-specific transcriptome was presented, comprising commonly expressed transcripts between hNESs derived from hESCs and ReNcell CX cells. To elucidate the molecular mechanisms associated with the hNSC identity, the hNSC-specific transcriptome was analyzed using pathway and functional annotation clustering analyses. The results suggested that hESC-derived hNESs, an expandable and accessible cell source, may be used as a relevant hNSC model in a wide range of neurological investigations.

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Section: Introductionmentioning

confidence: 99%

Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model

Jung

Lee

et al. 2017

Int J Mol Med

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“…The types of NSCs that have been used for experimental SCT include primary NSCs isolated from neurogenic regions of the brain, glial progenitors, immortalized clonal cell lines, and NSCs differentiated from ESCs or iPSCs. 11 Preclinical studies have shown therapeutic promise in several rodent disease models.…”

Section: Neural Stem Cellsmentioning

confidence: 99%

Stem Cell Therapy for the Central Nervous System in Lysosomal Storage Diseases

Siddiqi

Wolfe

2016

Human Gene Therapy

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Neurological diseases with genetic etiologies result in the loss or dysfunction of neural cells throughout the CNS. At present, few treatment options exist for the majority of neurogenetic diseases. Stem cell transplantation (SCT) into the CNS has the potential to be an effective treatment modality because progenitor cells may replace lost cells in the diseased brain, provide multiple trophic factors, or deliver missing proteins. This review focuses on the use of SCT in lysosomal storage diseases (LSDs), a large group of monogenic disorders with prominent CNS disease. In most patients the CNS disease results in intellectual disability that is refractory to current standard-of-care treatment. A large amount of preclinical work on brain-directed SCT has been performed in rodent LSD models. Cell types that have been used for direct delivery into the CNS include neural stem cells, embryonic and induced pluripotent stem cells, and mesenchymal stem cells. Hematopoietic stem cells have been an effective therapy for the CNS in a few LSDs and may be augmented by overexpression of the missing gene. Current barriers and potential strategies to improve SCT for translation into effective patient therapies are discussed.

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Stem Cell Applications in Lysosomal Storage Disorders: Progress and Ongoing Challenges

Köse

Aerts‐Kaya

Çetinkaya

et al. 2021

Advances in Experimental Medicine and Biology

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Pluripotent stem cell-derived neural stem cells: From basic research to applications

Cited by 7 publications

References 64 publications

Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model

Comparative analysis of human embryonic stem cell‑derived neural stem cells as an in vitro human model

Stem Cell Therapy for the Central Nervous System in Lysosomal Storage Diseases

Stem Cell Applications in Lysosomal Storage Disorders: Progress and Ongoing Challenges

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