Pluripotential Differentiation Capability of Human Adipose-derived Stem Cells in a Novel Fibrin-agarose Scaffold

Nieto-Aguilar, Renato; Serrato, Deyanira; Garzón, Ingrid; Campos, Antonìo; Alaminos, Miguel

doi:10.1177/0885328209360425

Cited by 20 publications

(20 citation statements)

References 43 publications

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“…To confirm the differentiation capability of the cells, 0.5 × 10 4 HWJSCs were placed on cell culture chamber slides for 4 weeks using osteogenic, adipogenic, and chondrogenic induction media, as we previously described [9]. The composition of these media is shown in supplemental online Table 1.…”

Section: Methodsmentioning

confidence: 99%

Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration

Garzón

Miyake

González‐Andrades

et al. 2013

Stem Cells Translational Medicine

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Perinatal stem cells such as human umbilical cord Wharton's jelly stem cells (HWJSCs) are excellent candidates for tissue engineering because of their proliferation and differentiation capabilities. However, their differentiation potential into epithelial cells at in vitro and in vivo levels has not yet been reported. In this work we have studied the capability of HWJSCs to differentiate in vitro and in vivo to oral mucosa and skin epithelial cells using a bioactive three-dimensional model that mimics the native epithelial-mesenchymal interaction. To achieve this, primary cell cultures of HWJSCs, oral mucosa, and skin fibroblasts were obtained in order to generate a three-dimensional heterotypical model of artificial oral mucosa and skin based on fibrin-agarose biomaterials. Our results showed that the cells were unable to fully differentiate to epithelial cells in vitro. Nevertheless, in vivo grafting of the bioactive three-dimensional models demonstrated that HWJSCs were able to stratify and to express typical markers of epithelial differentiation, such as cytokeratins 1, 4, 8, and 13, plakoglobin, filaggrin, and involucrin, showing specific surface patterns. Electron microscopy analysis confirmed the presence of epithelial cell-like layers and well-formed cell-cell junctions. These results suggest that HWJSCs have the potential to differentiate to oral mucosa and skin epithelial cells in vivo and could be an appropriate novel cell source for the development of human oral mucosa and skin in tissue engineering protocols.

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Section: Methodsmentioning

confidence: 99%

Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration

Garzón

Miyake

González‐Andrades

et al. 2013

Stem Cells Translational Medicine

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“…Estos cultivos se mantuvieron en medio de Dullbecco (DMEM) suplementado con suero bovino fetal al 10% (FCS), según la técnica descrita por NietoAguilar et al, 2011 (17).…”

Section: Generación De Tejido óSeounclassified

“…Tras ello se alicuotó la mezcla en placas de cultivo de 6 pocillos que se incubaron a 37ºC durante 24 horas hasta su total solidificación. Para inducir la diferenciación de estas células hacia la estirpe osteogénica y generar un tejido óseo artificial, los constructos se cultivaron en un medio inductor específico compuesto de DMEM suplementado con 10% de FCS, 100nM de dexametasona, 10nM β-glicerol-fosfato y 50mML de ácido ascórbico durante 21 días, como describió Nieto-Aguilar (17). Una vez transcurrido el periodo de diferenciación se extrajeron los tejidos artificiales de las superficies de cultivo y se sometieron a compresión plástica (nanoestructuración) utilizando un biorreactor específico según lo descrito (20).…”

Section: Generación De Tejido óSeounclassified

Evaluation of bone density in palatine bone defects after grafting of bone generated by tissue engineering in rabbits

Gámez¹,

Sevilla²,

Martínez³

et al. 2017

Actual. Med.

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Evaluación de la densidad ósea en defectos óseos palatinos tras injerto de tejido óseo generado mediante ingeniería tisular en conejos ResumenObjetivos. Se pretende determinar mediante TAC la densidad ósea tras la utilización de injertos óseos autólogos artificiales generados mediante ingeniería tisular y determinar la utilidad in vivo de estos materiales. Métodos. Se han utilizado 12 conejos machos (New Zeland White) de 3 semanas de edad. Se generó hueso a partir de células madre mesenquimales del tejido adiposo a las que, tras cultivo y expansión, se indujo diferenciación a la estirpe osteogénica y se combinaron con biomateriales de fibrina y agarosa. Este sustituto óseo fue injertado de forma autóloga en los animales de experimentación. Se establecieron tres grupos de estudio: Grupo I: se crea defecto óseo de 4mm y se implanta el tejido óseo autólogo cubriendo con colgajo mucoperióstico. Grupo II: Como control negativo, se realiza el mismo procedimiento, pero sin implantar tejido óseo. Grupo III: Como control positivo, conejos a los que no se realiza ningún procedimiento. Se estudió la densidad ósea a los 6 meses tras el implante mediante tomografía computerizada, expresadas en unidades Hounsfield, comparando ambos hemipaladares (lado intervenido y lado control) Resultados. En el grupo III control positivo, la densidad ósea era similar en ambos hemipaladares (p>0'05). Por el contrario, en el grupo II controles negativos, se observó una diferencia estadísticamente significativa en la densidad del hueso palatino derecho frente al izquierdo (p<0'05). En el grupo I. no se observaron diferencias estadísticamente significativas entra ambos lados del paladar (p>0'05). Conclusión. La tomografía computerizada y el análisis de los datos en unidades Hounsfield, sugiere que el tejido óseo generado por ingeniería tisular contribuye a mejorar la calidad y densidad ósea en defectos óseos palatinos creados en conejos de laboratorio. AbstractPurpose. We have carried out a study to determine the bone density after using artificial autologous bone grafts generated by tissue engineering by means of computed tomography, and to determine the in vivo usefulness of these biomaterials. Methods. Twelve 3-week-old New Zeland White male rabbits were used in this study. Bone substitutes were generated by using adipose tissue mesenchymal stem cells. After culture and expansion, osteogenic differentiation was induced and cells were combined with fibrin-agarose biomaterials. This bone substitute was autologously grafted in experimental animals. Three study groups were established: Group I: a 4mm bone defect was created and the autologous bone tissue was implanted, covered by a mucoperiosteal flap. Group II: as a negative control, the same procedure was performed, but without implanting bone tissue. Group III: as a positive control, native non-operated rabbits were used. Bone density was studied 6 months after implantation by using computed tomography, expressed in Hounsfield units, and both sides of the palate -operated and control side-wer...

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“…These cells can be autologously obtained in higher number, and are preferred for TE applications in comparison with other sources of MSCs [31,32,70,165,188,[196][197][198]. ADMSCs were differentiated into SC phenotype and compared with differentiated BMSCs and SC.…”

Section: Stem Cellsmentioning

confidence: 99%

Tissue engineering of the peripheral nervous system

Carriel¹,

Alaminos²,

Garzón³

et al. 2014

Expert Review of Neurotherapeutics

103

142

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The structure and function of peripheral nerves can be affected by a range of conditions with severe consequences in these patients. Currently, there are several surgical techniques available to treat peripheral nerve defects. Direct repair is the preferred treatment for short nerve gaps, and nerve autografting is the gold standard in critical nerve defects. The autografting is not always available, and the use of allograft, decellularized allograft and nerve conduits are often used with variable success. During the recent years, several outcomes were achieved in peripheral nerve tissue engineering. Promising experimental results have been demonstrated with this novel generation of nerve conduits, mainly composed by biodegradables materials in combination with intraluminal fillers, growth factors and different cell sources.

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Pluripotential Differentiation Capability of Human Adipose-derived Stem Cells in a Novel Fibrin-agarose Scaffold

Cited by 20 publications

References 43 publications

Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration

Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration

Evaluation of bone density in palatine bone defects after grafting of bone generated by tissue engineering in rabbits

Tissue engineering of the peripheral nervous system

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