Pluronic F127 and P104 Polymeric Micelles as Efficient Nanocarriers for Loading and Release of Single and Dual Antineoplastic Drugs

Abstract

The potential application of biodegradable and biocompatible polymeric micelles formed by Pluronic F127 and P104 as nanocarriers of the antineoplastic drugs docetaxel (DOCE) and doxorubicin (DOXO) is presented in this work. The release profile was carried out under sink conditions at 37 °C and analyzed using the Higuchi, Korsmeyer–Peppas, and Peppas–Sahlin diffusion models. The cell viability of HeLa cells was evaluated using the proliferation cell counting kit CCK-8 assay. The formed polymeric micelles solubilized significant amounts of DOCE and DOXO, and released them in a sustained manner for 48 h, with a release profile composed of an initial rapid release within the first 12 h followed by a much slower phase the end of the experiments. In addition, the release was faster under acidic conditions. The model that best fit the experimental data was the Korsmeyer–Peppas one and denoted a drug release dominated by Fickian diffusion. When HeLa cells were exposed for 48 h to DOXO and DOCE drugs loaded inside P104 and F127 micelles, they showed lower IC50 values than those reported by other researchers using polymeric nanoparticles, dendrimers or liposomes as alternative carriers, indicating that a lower drug concentration is needed to decrease cell viability by 50%.

Comparing PVP and Polymeric Micellar Formulations of a PEGylated Photosensitizing Phthalocyanine by NMR and Optical Techniques

“…The impact of triblock copolymer micelle mediated cell internalization of Pc1 remains to be studied. It is known that PEG–PPG–PEG micelles are taken up by cells including HeLa cells. ,, Based on the weak interactions observed here between Pc1 and P188, the results for P188 are expected to be similar to those obtained for pure PBS (Figure A). However, for Pc1-P84 and Pc1-F127, the relatively high intermolecular binding affinity is expected to yield sufficient intracellular Pc1 as found for the Pc1-RH40 and Pc1-PVP systems.…”

Formulation of silymarin surface modified vesicles: In vitro characterization to cell viability assessment

“…The absorbance of each well was measured at 570 nm using a microplate reader. IC 50 of each sample was calculated by fitting the results to a Graph Pad Prism software ( Gutierrez-Saucedo et al, 2023 ).…”

Formulation and Characterization of a Pluronic F127 Polymeric Micelle as a Nanocarrier for Berberine Delivery

“…In aqueous solutions, these polymers form micelles with a hydrophobic PPO core and a hydrophilic PEO corona that interface with water [13]. They improve the circulation time of loaded molecules as well as enhance the intracellular uptake of encapsulated molecules via endocytosis rather than simple diffusion [14]. Pluronic polymers are approved by the Federal Drug Administration as safe and biodegradable compounds [15].…”