Pluronic F127 self-assembled MoS₂ nanocomposites as an effective glutathione responsive anticancer drug delivery system

Abstract

In this study, bio-responsive polymeric MoS 2 nanocomposites were prepared for use as a drug carrier for cancer therapy. Herein, we report the synthesis and demonstrate the self-assembly of pluronic F127 (PF127) on a cystamine-glutathione-MoS 2 (CYS-GSH-MoS 2 ) system, which can be used for GSH-triggered drug release under biological reducing conditions. The reduction-sensitive disulfide bond containing CYS was incorporated between the amphiphilic copolymer PF127 and GSH-MoS 2 to achieve feasible drug release.Percent drug loading capacity and encapsulation efficiency were 51.3% and 56%, respectively. In addition, when the MoS 2 -GSH-CYS-PF127 nanocomposite was incubated in a GSH environment, the morphology of the nanocomposite tended to change, ultimately leading to drug release. The drug-loaded PF127-CYS-GSH-MoS 2 polymeric nanocomposites efficiently released 52% of their drug content after 72 h of incubation in a GSH reduction environment. The HeLa cells treated with DOX loaded MoS 2 -GSH-CYS-PF127 showed 38% toxicity at drug concentration of 40 mg, which indicated that the successfully released of drug from carrier and caused the cell death. Further, fluorescence microscopy images of HeLa cells revealed the potential behavior of the MoS 2 -GSH-CYS-PF12 nanocomposite during the 2-and 4 h incubation periods; the nanocomposite was only found in the cytoplasm of HeLa cells. Interestingly, after 6 h of incubation, the drug was slowly released from the nanocomposite and could enter the nucleus as confirmed by fluorescence imaging of HeLa cells. Altogether, our synthesized PF127-coated MoS 2 nanocomposite could be effectively adopted in the near future as a GSH-sensitive drug carrier.

Vacancies on 2D transition metal dichalcogenides elicit ferroptotic cell death

“…Under similar doses, same exposure time, and route, we and Wang et al discovered similar pulmonary inflammation effects for MoS 2 nanosheets 9 . Besides, consistent with our nano-SAR findings, the surface chemistry of TMDs may play a major role in their toxicities, evidenced by the increased biocompatibility of MoS 2 nanosheets functionalized by Pluronic 127 47 , Pluronic 87 9 and PEG molecules 48 .…”

Recent advances in the design of controlled- and sustained-release micro/nanocarriers of pesticide

“…In another study, a bioresponsive nanocomposite was prepared by using a reduction-sensitive disulfide bond containing cysteine to connect the amphiphilic copolymer Pluronic F127 to MoS 2 , in which MoS 2 was a nanocarrier modified with GSH. 97 This composite carrier loaded doxorubicin released the transport drug in a GSH reduction environment. In recent years, MoS 2 nanocarriers have also made great progress in agriculture.…”

Enhanced Cellular Uptake in an Electrostatically Interacting Fucoidan–L-Arginine Fiber Complex

“…Further, the energy-dispersive X-ray spectroscopy confirmation of elements is presented in the supplementary data (S2). Fiber morphology plays a significant role in cellular internalization through vertical overlap with the cell membrane [36,37] (Figure 3). supplementary data (S2).…”

“…supplementary data (S2). Fiber morphology plays a significant role in cellular internalization through vertical overlap with the cell membrane [36,37] (Figure 3).…”