Pluronic nanoparticles do not modulate immune responses mounted by macrophages

Kim, Hyun Gyung; Jo, Sang Hyun; Yeon, Seung Min; Kim, Kyong Hoon; Chung, Jin Woong; Park, Tae Won; Byun, Youngjoo; Lee, Eun Hee; Park, Young In; Jung, Yong Woo

doi:10.1007/s13233-013-1174-7

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…However, here we have demonstrated that molecules used to functionalize NCs might have diverse impacts on immune cell viability and activation, evidencing the need to know well, before any medical application, the expected action of different functionalized particles on immune cells. Pluro Chito and PEG coatings have been well investigated and characterized by many reports 20 58 59 60 . However, this is to our knowledge the first study comparing the action on immune cells of three highly used nanocapsule coatings for biomedical applications as it is well known in nanoscience the extreme importance of evaluating the immune response to different nanoformulations 61 .…”

Section: Discussionmentioning

confidence: 99%

Immune cell impact of three differently coated lipid nanocapsules: pluronic, chitosan and polyethylene glycol

Farace

Sánchez-Moreno

Orecchioni

et al. 2016

Sci Rep

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Lipid nanocapsules (NCs) represent promising tools in clinical practice for diagnosis and therapy applications. However, the NC appropriate functionalization is essential to guarantee high biocompatibility and molecule loading ability. In any medical application, the immune system-impact of differently functionalized NCs still remains to be fully understood. A comprehensive study on the action exerted on human peripheral blood mononuclear cells (PBMCs) and major immune subpopulations by three different NC coatings: pluronic, chitosan and polyethylene glycol-polylactic acid (PEG) is reported. After a deep particle characterization, the uptake was assessed by flow-cytometry and confocal microscopy, focusing then on apoptosis, necrosis and proliferation impact in T cells and monocytes. Cell functionality by cell diameter variations, different activation marker analysis and cytokine assays were performed. We demonstrated that the NCs impact on the immune cell response is strongly correlated to their coating. Pluronic-NCs were able to induce immunomodulation of innate immunity inducing monocyte activations. Immunomodulation was observed in monocytes and T lymphocytes treated with Chitosan-NCs. Conversely, PEG-NCs were completely inert. These findings are of particular value towards a pre-selection of specific NC coatings depending on biomedical purposes for pre-clinical investigations; i.e. the immune-specific action of particular NC coating can be excellent for immunotherapy applications.

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Section: Discussionmentioning

confidence: 99%

Immune cell impact of three differently coated lipid nanocapsules: pluronic, chitosan and polyethylene glycol

Farace

Sánchez-Moreno

Orecchioni

et al. 2016

Sci Rep

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“…Pluronic is a nonionic three-block copolymer composed of a central hydrophobic chain of polyoxypropylene flanked by hydrophilic polyoxyethylene (PEO-PPO-PEO) that can be used as the carrier of hydrophobic drugs that interact with the hydrophobic PPO block [ 24 ]. Pluronic has also been recognized as a long-circulating, anti-metastases and anti-chemoresistance agent thanks to its strong effect on changing tumor viscosity and inhibiting multiple drug-resistant proteins [ [25] , [26] , [27] , [28] ]. Although pH-responsive curcumin release systems based on pluronic have been studied before, its thermo-responsive behavior is remained relatively unexplored [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Controlled temperature-mediated curcumin release from magneto-thermal nanocarriers to kill bone tumors

Khodaei

Jahanmard

Hosseini

et al. 2022

Bioactive Materials

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Systemic chemotherapy has lost its position to treat cancer over the past years mainly due to drug resistance, side effects, and limited survival ratio. Among a plethora of local drug delivery systems to solve this issue, the combinatorial strategy of chemo-hyperthermia has recently received attention. Herein we developed a magneto-thermal nanocarrier consisted of superparamagnetic iron oxide nanoparticles (SPIONs) coated by a blend formulation of a three-block copolymer Pluronic F127 and F68 on the oleic acid (OA) in which Curcumin as a natural and chemical anti-cancer agent was loaded. The subsequent nanocarrier SPION@OA-F127/F68-Cur was designed with a controlled gelation temperature of the shell, which could consequently control the release of curcumin. The release was systematically studied as a function of temperature and pH, via response surface methodology (RSM). The bone tumor killing efficacy of the released curcumin from the carrier in combination with the hyperthermia was studied on MG-63 osteosarcoma cells through Alamar blue assay, live-dead staining and apoptosis caspase 3/7 activation kit. It was found that the shrinkage of the F127/F68 layer stimulated by elevated temperature in an alternative magnetic field caused the curcumin release. Although the maximum release concentration and cell death took place at 45 °C, treatment at 41 °C was chosen as the optimum condition due to considerable cell apoptosis and lower side effects of mild hyperthermia. The cell metabolic activity results confirmed the synergistic effects of curcumin and hyperthermia in killing MG-63 osteosarcoma cells.

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“Omic” Techniques for Nanosafety

Feng

2016

Toxicology of Nanomaterials

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Pluronic nanoparticles do not modulate immune responses mounted by macrophages

Cited by 6 publications

References 28 publications

Immune cell impact of three differently coated lipid nanocapsules: pluronic, chitosan and polyethylene glycol

Immune cell impact of three differently coated lipid nanocapsules: pluronic, chitosan and polyethylene glycol

Controlled temperature-mediated curcumin release from magneto-thermal nanocarriers to kill bone tumors

“Omic” Techniques for Nanosafety

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