PlWRKY13: A Transcription Factor Involved in Abiotic and Biotic Stress Responses in Paeonia lactiflora

Abstract

Many members of the WRKY family regulate plant growth and development. Recent studies have shown that members of the WRKY family, specifically WRKY13, play various roles in the regulation of plant stress resistance. To study the function of WRKY family members in peony, the PlWRKY13 gene (KY271095) was cloned from peony leaves. Sequence analysis and subcellular localization results revealed that PlWRKY13 has no introns, belongs to the type IIc subgroup of the WRKY family, and functions in the nucleus. The expression pattern of PlWRKY13 was analysed via real-time quantitative RT-PCR (qRT-PCR), which showed that the expression of PlWRKY13 was induced by four types of abiotic stress, low-temperature, high-temperature, waterlogging and salt stress, and was positively upregulated in response to these stresses. In addition, the expression of PlWRKY13 tended to first decrease and then increase after infection with Alternaria tenuissima. Virus-induced gene silencing (VIGS) technology was used to explore the function of PlWRKY13 in the resistance of Paeonia lactiflora to fungal infection further, and the results showed that PlWRKY13-silenced plants displayed increased sensitivity to A. tenuissima. The infection was more severe and the disease index (DI) significantly greater in the PlWRKY13-silenced plants than in the control plants, and the expression of pathogenesis-related (PR) genes was also significantly altered in the PlWRKY13-silenced plants compared with the control plants. The contents of the endogenous hormones jasmonic acid (JA) and salicylic acid (SA) were measured, and the results showed that the JA content increased gradually after infection with A. tenuissima and that JA may play an active role in the resistance of P. lactiflora to pathogen infection, while the SA content decreased after PlWRKY13 silencing. The contents of the two hormones decreased overall, suggesting that they are related to the transcription of PlWRKY13 and that PlWRKY13 may be involved in the disease-resistance pathway mediated by JA and SA. In summary, the results of our study showed that PlWRKY13 expression was induced by stress and had a positive effect on the resistance of P. lactiflora to fungal infection.

Small protein complex prediction algorithm based on protein–protein interaction network segmentation

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Germplasm resources and genetic breeding of Paeonia: a systematic review

“…The expression of nucleus-located PlWRKY13, which was also cloned from P. lactiflora 'Da Fugui', was upregulated by four abiotic stresses: low and high temperatures, waterlogging, and salt stress. In addition, PlWRKY13-silenced plants were more sensitive to fungal infection, indicating that PlWRKY13 was induced by abiotic stresses but involved in the regulation of disease resistance 181 . PsDREB is a tree peony transcription factor related to stress resistance.…”

Physiological and Gene Expression Analysis of Herbaceous Peony Resistance to Alternaria tenuissima Infection

“…So far, limited reports exist on the physiological and molecular responses of peonies to the pathogen Alternaria. Only recently, two WRKYs (PlWRKY13 and PlWRKY65) and five PRs (PlPR1, PlPR2, PlPR4B, PlPR5, and PlPR10) were found to be involved in peony disease resistance [18,19]. Therefore, the physiological parameters and the expression of these seven genes were compared between the disease-resistant (R) and susceptible (S) varieties against the pathogen A. tenuissima.…”