Pm21 from Haynaldia villosa Encodes a CC-NBS-LRR Protein Conferring Powdery Mildew Resistance in Wheat

Xing, Liping; Hu, Ping; Liu, Jiaqian; Witek, Kamil; Zhou, Shuang; Xu, Jiefei; Zhou, Weihao; Gao, Li; Huang, Zhenpu; Zhang, Ruiqi; Wang, Xiue; Chen, Peidu; Wang, Haiyan; Jones, Jonathan D. G.; Karafiátová, Miroslava; Vrána, Jan; Bartoš, Jan; Doležel, Jaroslav; Tian, Yuanchun; Wu, Yufeng; Cao, Aizhong

doi:10.1016/j.molp.2018.02.013

Cited by 222 publications

(164 citation statements)

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“…This hypothesis was supported by the evidence that Stpk‐V silencing did not completely compromise resistance of Pm21 (Cao et al , 2011). Recent work further substantiates the hypothesis where two typical NLR genes, DvRGA2 (He et al , 2018) and NLR1‐V (Xing et al , 2018), were found as candidates for Pm21 and are probably allelic.…”

Section: Challenges In Current Resistance Breedingsupporting

confidence: 61%

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

et al. 2020

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Powdery mildew (Blumeria graminis f. sp. tritici) results in serious economic loss in wheat production. Exploration of plant resistance to wheat powdery mildew over several decades has led to the discovery of a wealth of resistance genes and quantitative trait loci (QTLs). We have provided a comprehensive summary of over 200 powdery mildew genes (permanently and temporarily designated genes) and QTLs reported in common bread wheat. This highlights the diverse and rich resistance sources that exist across all 21 chromosomes. To manage different data for breeders, here we also present a bridged mapping result from previously reported powdery mildew resistance genes and QTLs with the application of a published integrated wheat map. This will provide important insights to empower further breeding of powdery mildew resistant wheat via marker‐assisted selection (MAS).

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Section: Challenges In Current Resistance Breedingsupporting

confidence: 61%

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

et al. 2020

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“…NBS-LRR R genes reportedly recognized pathogens and initiated defense responses subsequently. To date, five out of 89 Pm genes identified in wheat gene pool, such as Pm2 [49], Pm3 [50], Pm8 [51], Pm21 [28,52] and Pm60 [9], have been cloned, which all encoded CC-NBS-LRR proteins. So CC-NBS-LRR genes identification could provide a promising strategy to isolate candidate R genes.…”

Section: Discussionmentioning

confidence: 99%

Discovery of powdery mildew resistance gene candidates fromAegilops biuncialischromosome 2M^bbased on transcriptome sequencing

Zhang

et al. 2019

Preprint

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Powdery mildew is one of the most widespread diseases of wheat. Breeding resistant varieties by utilization of resistance genes is considered as the most economic and effective method of controlling this disease. Previous study showed that the gene(s) at 2Mb in Chinese Spring (CS)-Aegilops biuncialis 2Mb disomic addition line TA7733 conferred high resistance to powdery mildew. In this study, 15 Bgt isolates prevalent in different regions of China were used to further test the resistance spectrum of TA7733. As a result, TA7733 was high resistance to all tested isolates, indicating that the gene(s) on chromosome 2Mb was broad-spectrum powdery mildew resistance. In order to mine resistance gene candidates and develop 2Mb-specific molecular markers to assist the transfer resistance gene(s) at chromosome 2Mb, RNA-seq of TA7733 and CS was conducted before and after Bgt-infection, generating a total of 158,953 unigenes. Of which, 7,278 unigenes were TA7733-specific which were not expressed in CS, and 295 out of these 7,278 unigenes were annotated as R genes. Based on Blastn against with CS Ref Seq v1.0, 61 R genes were further mapped to homoeologous group 2. Analysis of R gene-specific molecular markers designed from R gene sequences verified 40 out of 61 R genes to be 2Mb specific. Annotation of these 40 R genes showed most genes encoded nucleotide binding leucine rich repeat (NLR) protein, being most likely resistance gene candidates. The broad-spectrum powdery mildew resistance gene(s), disease resistance gene candidates, and functional molecular markers of 2Mb-specific in present study will not only lay foundations for transferring disease resistance gene(s) from 2Mb to common wheat by inducing CS-Ae. biuncialis homoeologous recombination, but also provide useful candidates for isolating and cloning resistance gene(s) and dissecting molecular and genetic mechanisms of disease resistance from 2Mb.

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“…Therefore, these desirable genes related to target traits should be separated from derivatives between wheat and wild relatives. Advanced biotechnologies, such as next-generation sequencing and homology-based cloning, have proven beneficial in accelerating gene discovery directly from derived lines of wheat and wild relatives [16,56,57]. However, most of these studies related to the cloning of genes from derived lines have focused on disease-resistant genes, and no relevant studies have reported the cloning of genes associated with complex traits such as yield directly from derived lines.…”

Section: Tissue-enriched Transcripts Expression and Enrichment Analysmentioning

confidence: 99%

“…Comprehensive approaches, including cytogenetic stock development, mutagenesis, resistance gene enrichment and sequencing-Pacific Biosciences (PacBio), long-range assembly, and functional analysis, were successively used to successfully clone the Pm21 gene, which confers high resistance to Blumeria graminis f. sp. tritici (Bgt) in wheat throughout all growth stages, from the wild species Haynaldia villosa [16]. At the same time, Pm21 was also isolated and functionally validated via the discovery of Bgt-susceptible D. villosum resources and construction of a genetic population using resistant intervals [17].…”

Section: Introductionmentioning

confidence: 99%

Full-length transcriptome sequences of Agropyron cristatum facilitate the prediction of putative genes for thousand-grain weight in a wheat-A. cristatum translocation line

Zhou

Zhang

Han

et al. 2019

Preprint

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Pm21 from Haynaldia villosa Encodes a CC-NBS-LRR Protein Conferring Powdery Mildew Resistance in Wheat

Cited by 222 publications

References 10 publications

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

Discovery of powdery mildew resistance gene candidates fromAegilops biuncialischromosome 2M^bbased on transcriptome sequencing

Full-length transcriptome sequences of Agropyron cristatum facilitate the prediction of putative genes for thousand-grain weight in a wheat-A. cristatum translocation line

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Pm21 from Haynaldia villosa Encodes a CC-NBS-LRR Protein Conferring Powdery Mildew Resistance in Wheat

Cited by 222 publications

References 10 publications

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

Discovery of powdery mildew resistance gene candidates fromAegilops biuncialischromosome 2Mbbased on transcriptome sequencing

Full-length transcriptome sequences of Agropyron cristatum facilitate the prediction of putative genes for thousand-grain weight in a wheat-A. cristatum translocation line

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Discovery of powdery mildew resistance gene candidates fromAegilops biuncialischromosome 2M^bbased on transcriptome sequencing