PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin

Shapoval, Oleksandr; Brandmeier, Julian C.; Nahorniak, Mykhailo; Oleksa, Viktoriia; Makhneva, Ekaterina; Gorris, Hans H.; Horák, Daniel

doi:10.1016/j.talanta.2022.123400

Cited by 13 publications

(16 citation statements)

References 45 publications

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“…Since the synthesized UCNPs are hydrophobic, their modification by a hydrophilic PMVEMA coating is necessary to ensure their functionality, colloidal and chemical stability in physiological media, and conjugation with THPC [ 27 , 38 ]. The presence of the PMVEMA containing carboxyl groups on the particle surface was accompanied by a change in the ζ-potential, which became negative (−29 mV; Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

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Temoporfin-Conjugated Upconversion Nanoparticles for NIR-Induced Photodynamic Therapy: Studies with Pancreatic Adenocarcinoma Cells In Vitro and In Vivo

Shapoval,

Větvička,

Patsula

et al. 2023

Pharmaceutics

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Upconverting nanoparticles are interesting materials that have the potential for use in many applications ranging from solar energy harvesting to biosensing, light-triggered drug delivery, and photodynamic therapy (PDT). One of the main requirements for the particles is their surface modification, in our case using poly(methyl vinyl ether-alt-maleic acid) (PMVEMA) and temoporfin (THPC) photosensitizer to ensure the colloidal and chemical stability of the particles in aqueous media and the formation of singlet oxygen after NIR irradiation, respectively. Codoping of Fe2+, Yb3+, and Er3+ ions in the NaYF4 host induced upconversion emission of particles in the red region, which is dominant for achieving direct excitation of THPC. Novel monodisperse PMVEMA-coated upconversion NaYF4:Yb3+,Er3+,Fe2+ nanoparticles (UCNPs) with chemically bonded THPC were found to efficiently transfer energy and generate singlet oxygen. The cytotoxicity of the UCNPs was determined in the human pancreatic adenocarcinoma cell lines Capan-2, PANC-01, and PA-TU-8902. In vitro data demonstrated enhanced uptake of UCNP@PMVEMA-THPC particles by rat INS-1E insulinoma cells, followed by significant cell destruction after excitation with a 980 nm laser. Intratumoral administration of these nanoconjugates into a mouse model of human pancreatic adenocarcinoma caused extensive necrosis at the tumor site, followed by tumor suppression after NIR-induced PDT. In vitro and in vivo results thus suggest that this nanoconjugate is a promising candidate for NIR-induced PDT of cancer.

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Section: Resultsmentioning

confidence: 99%

“…PMVEMA-coated UCNPs (UCNP@PMVEMA) were prepared according to the previously described procedure [ 27 ]. Briefly, PMVEMA (250 mg) was dissolved in water (5 mL) at RT, and the pH was adjusted to 7.4 using 2 M NaOH.…”

Section: Methodsmentioning

confidence: 99%

Temoporfin-Conjugated Upconversion Nanoparticles for NIR-Induced Photodynamic Therapy: Studies with Pancreatic Adenocarcinoma Cells In Vitro and In Vivo

Shapoval,

Větvička,

Patsula

et al. 2023

Pharmaceutics

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“…[143,144] These biomarkers serve as antigens that can be captured by corresponding antibodies on the nanomaterial surface, facilitating the in vitro diagnosis of CVD (Table 1). [145,146]…”

Section: Osteopontinmentioning

confidence: 99%

Nanomaterials Responsive to Endogenous Biomarkers for Cardiovascular Disease Theranostics

Wei

Jiang

et al. 2023

Adv Funct Materials

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Cardiovascular disease (CVD) is the number one cause of death worldwide, which propels the development of advanced technologies for CVD diagnosis and treatment. Biomarker-responsive nanomaterials are appealing therapeutic platforms that provide new horizons for CVD theranostics. In this review, recent advances in nanomaterials with endogenous biomarkers as stimuli or targets for CVD theranostics is presented. First, the categories of biomarkers involved are comprehensively itemized based on pathological mechanisms including pH, reactive oxygen species, lipids, enzymes, macrophage receptors, subendothelium components, platelet receptors, inflammation, and osteopontin. The role of these biomarkers in bridge-building between nanomaterials and CVD is then presented. Next, the biomedical applications of nanomaterials responsive to endogenous biomarkers as stimuli or targets for the diagnosis and treatment of CVD are elaborated. Finally, the challenges and future research directions of biomarker-responsive nanomaterials in CVD are also discussed. This review will provide scientific guidance to facilitate clinical applications of biomarker-responsive nanomaterials.

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“…Therefore, they are more suitable as fluorescent probes in biosensing applications than conventionally used organic dyes and quantum dots. − ,− In addition, the UCL properties of UCNPs are inert to changes in buffer composition, sample matrix, and other environmental conditions . UCNP-based bioassay research is becoming a flourishing field and various types of UCNP-based bioassays have emerged over the past two decades. − ,− Sandwich-type heterogeneous biosensors are frequently developed because the use of a matched pair of antibodies provides the highest level of sensitivity and specificity. ,− Many studies have aimed to develop sandwich-type heterogeneous UCNP-based biosensors suitable for clinical and hospital diagnostic tools. − …”

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confidence: 99%

“…29 Due to the low concentration of cTnI in blood, an ultrasensitive assay is required to accurately quantify cTnI. However, the LODs of most sandwich-type heterogeneous UCNP-based biosensors are still in pg/mL to tens of ng/mL range, [17][18][19][20][21]23 which hinders their application in diagnosis of myocardial infarction and cardiotoxicity. Hence, there is a need to further improve the LOD of sandwich-type heterogeneous UCNP-based biosensors.…”

mentioning

confidence: 99%

Ultrasensitive Upconversion Nanoparticle Immunoassay for Human Serum Cardiac Troponin I Detection Achieved with Resonant Waveguide Grating

Tseng,

Chiu,

Pham

et al. 2024

ACS Sens.

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Selective detection of biomarkers at low concentrations in blood is crucial for the clinical diagnosis of many diseases but remains challenging. In this work, we aimed to develop an ultrasensitive immunoassay that can detect biomarkers in serum with an attomolar limit of detection (LOD). We proposed a sandwich-type heterogeneous immunosensor in a 3 × 3 well array format by integrating a resonant waveguide grating (RWG) substrate with upconversion nanoparticles (UCNPs). UCNPs were used to label a target biomarker captured by capture antibody molecules immobilized on the surface of the RWG substrate, and the RWG substrate was used to enhance the upconversion luminescence (UCL) of UCNPs through excitation resonance. The LOD of the immunosensor was greatly reduced due to the increased UCL of UCNPs and the reduction of nonspecific adsorption of detection antibody-conjugated UCNPs on the RWG substrate surface by coating the RWG substrate surface with a carboxymethyl dextran layer. The immunosensor exhibited an extremely low LOD [0.24 fg/mL (9.1 aM)] and wide detection range (1 fg/mL to 100 pg/mL) in the detection of cardiac troponin I (cTnI). The cTnI concentrations in human serum samples collected at different times during cyclophosphamide, epirubicin, and 5fluorouracil (CEF) chemotherapy in a breast cancer patient were measured by an immunosensor, and the results showed that the CEF chemotherapy did cause cardiotoxicity in the patient. Having a higher number of wells in such an array-based biosensor, the sensor can be developed as a high-throughput diagnostic tool for clinically important biomarkers.

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PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin

Cited by 13 publications

References 45 publications

Temoporfin-Conjugated Upconversion Nanoparticles for NIR-Induced Photodynamic Therapy: Studies with Pancreatic Adenocarcinoma Cells In Vitro and In Vivo

Temoporfin-Conjugated Upconversion Nanoparticles for NIR-Induced Photodynamic Therapy: Studies with Pancreatic Adenocarcinoma Cells In Vitro and In Vivo

Nanomaterials Responsive to Endogenous Biomarkers for Cardiovascular Disease Theranostics

Ultrasensitive Upconversion Nanoparticle Immunoassay for Human Serum Cardiac Troponin I Detection Achieved with Resonant Waveguide Grating

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