Pneumatic Air Separation for the Sorting of Parasitized and Unparasitized Fruit Fly (Diptera: Tephritidae) Puparia

Jackson, C. G.; Chan, Harvey T.; Taniguchi, Myles H.; Churchill, D. B.; Bilsland, D. M.

doi:10.1093/jee/89.2.353

Cited by 8 publications

(6 citation statements)

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“…Another pupa separation method is pneumatic air separation, which has been successfully used to separate unparasitized pupae of Bactrocera dorsalis , Bactrocera cucurbitae , and Ceratitis capitata from puparia parasitized by Diachasmimorpha longicaudata , Diachasmimorpha tryoni , and Psyttalia fletcheri . This method separates pupae in a stream of air, depending on their different bulk densities (weight to volume) (Jackson et al., 1996).…”

Section: Discussionmentioning

confidence: 99%

Automated egg‐collecting and pupa‐separator system for medfly mass‐rearing facilities

Morávek¹,

Kozánek²,

Vidlička³

et al. 2008

Entomologia Exp Applicata

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The new automated egg‐collecting system and pupa‐separator device described here substantially reduces labour during medfly [Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)] mass production. The egg‐collecting system gathers eggs dropping from the cage's oviposition net into a continuous stream of water, after which they accumulate in an egg‐collecting sieve. The system provides an optimal environment for eggs, keeping them in a slow stream of well‐oxygenated water. A pupa separator was designed to enable the separation of pupae from the pupation medium by sucking off the medium through a slowly moving sieving belt. The smaller particles of pupation medium are removed by a vacuum cleaner located under the circular sieving belt, and the pupae are collected in a collecting box. Quality control tests of the eggs collected and pupae separated have shown that neither system has a negative effect on the quality of the eggs or the pupae.

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Section: Discussionmentioning

confidence: 99%

Automated egg‐collecting and pupa‐separator system for medfly mass‐rearing facilities

Morávek¹,

Kozánek²,

Vidlička³

et al. 2008

Entomologia Exp Applicata

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“…A shorter time spent as a larva means less time to add body weight, and consequently the formation of lighter puparia. Such separators use an air stream (generated by an adjustable fan) to lift lighter puparia (males) to a collecting vessel at the top of the machine, while heavier puparia (females) sink to a lower chamber (Bautista et al, 1999 andJackson et al, 1996). It is also worth pointing out that the female puparia (for both larval feeding regimes) were significantly heavier than males.…”

Section: Pupal Stagementioning

confidence: 99%

“…In addition, where funds permit, an air stream separator could be used as an automated method of separating the adults by sex prior to emergence, thereby reducing the need for manual labour and consequently lowering the costs of a mass-rearing system for S. s. spinipes. Such separators use an air stream (generated by an adjustable fan) to lift lighter puparia (males) to a collecting vessel at the top of the machine, while heavier puparia (females) sink to a lower chamber (Bautista et al, 1999 andJackson et al, 1996). The median duration of the pupal stage was significantly shorter at the higher temperatures (23/26 °C) than at the lower temperatures (14/17 °C) for both larval feeding regimes.…”

Section: Pupal Stagementioning

confidence: 99%

Sexual dimorphism and the impact of temperature on the pupal and adult stages of Sepedon spinipes spinipes, a potential biological control agent of fascioliasis

McDonnell

Mulkeen

Gormally

2005

Entomologia Exp Applicata

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The malacophagous feeding behaviour of larvae of members of the family Sciomyzidae makes the group ideal for the selection of potential biological control agents of snail hosts of trematode diseases. The aim of this study was to investigate the effects of temperature (14 °C, 17 °C, 20 °C, 23 °C, and 26 °C) on the pupal and adult stages of Sepedon spinipes spinipes (Scopoli) (Diptera: Sciomyzidae). Larval prey density effects on the pupal stage and the extent of sexual dimorphism within the species are also assessed. Optimum growth conditions for the pupal stage and pre-oviposition/oviposition periods, and female fecundity are identified for mass culturing purposes. The tendency of mature third instar larvae to engage in wandering behaviour is highlighted, and the biological control implications of such behaviour are discussed. Female puparia were significantly (P<0.001) heavier than males for both larval prey densities. Such sexual dimorphism would enable the prediction of adult sex from puparial weight prior to eclosion, thereby facilitating culture establishment. The effect of three food types [honey/yeast mixture only, crushed Radix balthica (L.) (Gastropoda: Pulmonata: Lymnaeidae) only, and honey/yeast mixture plus a crushed snail] on adult longevity (laboratory conditions) was also determined and the results suggest that adults live longest when provided with a crushed snail and a honey/yeast mixture.

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“…Historically, various methods had been proposed to separate parasitoids from hosts. For example, in some instances the developmental rates of wasps and flies were sufficiently different to allow adult hosts to emerge first, die and leave parasitoids to emerge alone from parasitized pupae [ 31 ]. However, in the majority of cases, some handling was necessary.…”

Section: Introductionmentioning

confidence: 99%

Application of Nuclear Techniques to Improve the Mass Production and Management of Fruit Fly Parasitoids

Cancino

Ruiz

Viscarret

et al. 2012

Insects

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The use of irradiated hosts in mass rearing tephritid parasitoids represents an important technical advance in fruit fly augmentative biological control. Irradiation assures that fly emergence is avoided in non-parasitized hosts, while at the same time it has no appreciable effect on parasitoid quality, i.e., fecundity, longevity and flight capability. Parasitoids of fruit fly eggs, larvae and pupae have all been shown to successfully develop in irradiated hosts, allowing a broad range of species to be shipped and released without post-rearing delays waiting for fly emergence and costly procedures to separate flies and wasps. This facilitates the early, more effective and less damaging shipment of natural enemies within hosts and across quarantined borders. In addition, the survival and dispersal of released parasitoids can be monitored by placing irradiated sentinel-hosts in the field. The optimal radiation dosages for host-sterility and parasitoid-fitness differ among species, and considerable progress has been made in integrating radiation into a variety of rearing procedures.

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Pneumatic Air Separation for the Sorting of Parasitized and Unparasitized Fruit Fly (Diptera: Tephritidae) Puparia

Cited by 8 publications

References 0 publications

Automated egg‐collecting and pupa‐separator system for medfly mass‐rearing facilities

Automated egg‐collecting and pupa‐separator system for medfly mass‐rearing facilities

Sexual dimorphism and the impact of temperature on the pupal and adult stages of Sepedon spinipes spinipes, a potential biological control agent of fascioliasis

Application of Nuclear Techniques to Improve the Mass Production and Management of Fruit Fly Parasitoids

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