Pneumococcal Capsular Polysaccharide and Antibody in Pleural Exudates

Gara, Paul F. de; Bullowa, Jesse G. M.; Bukantz, Samuel C.

doi:10.1097/00000441-194203000-00011

Cited by 4 publications

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“…The fact that antigenemia with specific capsular polysaccharide occurs in some humans with pneumococcal pneumonia has been long established (5,6,7,9,10). However neither the frequency nor the duration of antigenemia in pneumococcal pneumonia is clearly understood.…”

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Correlation of Circulating Capsular Polysaccharide with Bacteremia in Pneumococcal Pneumonia

et al. 1972

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Immunoelectroosmophoresis with rabbit anticapsular antibody was used to detect type-specific pneumococcal polysaccharide in serum from bacteremic patients with pneumococcal pneumonia. The test could detect as little as 0.1 to 1.0 μg per ml or 0.2 to 2 ng per test of polysaccharide from types 1, 2, 3, 4, 5, 8, 12, and 18, and its sensitivity was 10 times that of double immunodiffusion. Although antigen could be detected by double immunodiffusion with types 7 and 14, no antigen could be detected by immunoelectroosmophoresis. Types 7 and 14 polysaccharides were found to be positively charged, whereas the other polysaccharides were negatively charged. Forty-six patients with pneumonia were selected for study because pneumococci corresponding to those types where the test was known to work had been isolated from blood or the respiratory tract. Antigenemia correlated strongly with bacteremia: 12 of 20 bacteremic patients with pneumonia showed antigenemia, whereas 26 patients negative for bacteremia did not show circulating antigen detectable with antisera against the pneumococcal type isolated from the respiratory tract. The apparent concentration of circulating polysaccharide ranged from 0.1 to 100 μg per ml of serum, and the concentration did not appear to diminish appreciably in 10 to 15 days. Three of 12 patients with antigenemia died, and two of these had the highest levels of circulating antigen observed.

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confidence: 99%

Correlation of Circulating Capsular Polysaccharide with Bacteremia in Pneumococcal Pneumonia

et al. 1972

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“…Cerebrospinal fluid containing capsular polysaccharide in low concentration may have to be added in large amounts to produce a visible precipitin reaction. In the presence of unusually high concentrations of capsular polysaccharide, the precipitin reaction may possibly be inhibited by virtue of entering the zone of large antigen excess (12).…”

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confidence: 99%