Pneumonia Due to Pseudomonas aeruginosa

Fujitani, Shigeki; Sun, Hsin‐Yun; Yu, Victor L.; Weingarten, Jeremy A.

doi:10.1378/chest.10-0166

Cited by 225 publications

(101 citation statements)

References 125 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Data were pooled from two independent experiments (values for n indicate the total numbers of mice). The statistical significance of differences in survival curves with respect to the WT are indicated as follows (Mantel-Cox test): *, P Ͻ 0.05; and ****, P Ͻ 0.0001. aeruginosa pneumonia in humans, which is associated with high mortality and can eventually degenerate into septicemia (1,2). This pathological situation differs greatly from chronic lung infections established by P. aeruginosa in CF patients, which can partly be mimicked in mice by using pathoadaptive CF isolates capable of persisting in the lungs without causing death (34).…”

Section: Discussionmentioning

confidence: 99%

“…Among hospital-acquired P. aeruginosa infections, ventilator-associated pneumonia is a major clinical entity, with Ϸ20% prevalence and dramatically high mortality and monetary costs (1,2). Other common syndromes associated with P. aeruginosa lung infection are those occurring in patients with chronic lung disease, such as chronic obstructive pulmonary disease and CF (3).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Role of Iron Uptake Systems in Pseudomonas aeruginosa Virulence and Airway Infection

et al. 2016

View full text Add to dashboard Cite

Pseudomonas aeruginosa is a leading cause of hospital-acquired pneumonia and chronic lung infections in cystic fibrosis patients. Iron is essential for bacterial growth, and P. aeruginosa expresses multiple iron uptake systems, whose role in lung infection deserves further investigation. P. aeruginosa Fe 3؉ uptake systems include the pyoverdine and pyochelin siderophores and two systems for heme uptake, all of which are dependent on the TonB energy transducer. P. aeruginosa also has the FeoB transporter for Fe 2؉ acquisition. To assess the roles of individual iron uptake systems in P. aeruginosa lung infection, single and double deletion mutants were generated in P. aeruginosa PAO1 and characterized in vitro, using iron-poor media and human serum, and in vivo, using a mouse model of lung infection. The iron uptake-null mutant (tonB1 feoB) and the Fe 3؉ transport mutant (tonB1) did not grow aerobically under low-iron conditions and were avirulent in the mouse model. Conversely, the wild type and the feoB, hasR phuR (heme uptake), and pchD (pyochelin) mutants grew in vitro and caused 60 to 90% mortality in mice. The pyoverdine mutant (pvdA) and the siderophore-null mutant (pvdA pchD) grew aerobically in iron-poor media but not in human serum, and they caused low mortality in mice (10 to 20%). To differentiate the roles of pyoverdine in iron uptake and virulence regulation, a pvdA fpvR double mutant defective in pyoverdine production but expressing wild-type levels of pyoverdine-regulated virulence factors was generated. Deletion of fpvR in the pvdA background partially restored the lethal phenotype, indicating that pyoverdine contributes to the pathogenesis of P. aeruginosa lung infection by combining iron transport and virulence-inducing capabilities.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Role of Iron Uptake Systems in Pseudomonas aeruginosa Virulence and Airway Infection

et al. 2016

View full text Add to dashboard Cite

show abstract

“…P. aeruginosa is a nosocomial pathogen. Nosocomial infections are hospital-acquired infections developed during treatment in hospitals or other health care service units (17,35). Unfortunately, P. aeruginosa has the ability to develop resistance to multiple classes of antibacterial-effective drugs, so that treatment of an infection has become a serious clinical problem (21).…”

Section: Discussionmentioning

confidence: 99%

“…Pseudomonas aeruginosa is an opportunistic pathogen of immunocompromised humans which typically infects the pulmonary tract, causing bacterial pneumonia (17). Notably, hospital-acquired pneumonia in mechanically ventilated patients is associated with a particularly high mortality rate (18,19).…”

mentioning

confidence: 99%

Pseudomonas aeruginosa and Its Bacterial Components Influence the Cytokine Response in Thymocytes and Splenocytes

et al. 2016

View full text Add to dashboard Cite

Infections with Pseudomonas aeruginosa may cause many different diseases. The spectrum of such infections in general includes inflammation and bacterial sepsis. Hospital-acquired pneumonia, naturally resistant to a wide range of antibiotics, is associated with a particularly high mortality rate in mechanically ventilated patients. The pathogenesis of P. aeruginosa is complex and mediated by several virulence factors, as well as cell-associated factors. We have previously demonstrated that stimulation with different bacteria triggers the cytokine response of thymocytes. In this study, we investigated the effect of P. aeruginosa and its different components on the cytokine production of immature and mature immune cells. We found that the induced cytokine pattern in the thymus and the spleen after infections with P. aeruginosa is primarily mediated by lipopolysaccharide (LPS) of the outer cell membrane, but other components of the bacterium can influence the cytokine secretion as well. Stimulation with heatkilled P. aeruginosa and LPS does not influence the amount of cytokine-producing CD4 ؉ T cells but instead suppresses the emergence of Th17 cells. However, stimulation with P. aeruginosa or its components triggers the interleukin-17 (IL-17) response both in thymocytes and in splenocytes. We conclude that infections with P. aeruginosa affect the cytokine secretion of immature and mature cells and that IL-17 and Th17 cells play only a minor role in the development of pathological systemic inflammatory disease conditions during P. aeruginosa infections. Therefore, other inflammatory immune responses must be responsible for septic reactions of the host. Interleukin-17 (IL-17) is a potent inflammatory cytokine, which was initially reported to be produced mainly by activated memory T cells, also called T helper (Th) 17 cells (1). In addition to induced IL-17-producing T cells, which are primed in the immune periphery during the induction of an antigen-specific T cell response, these IL-17-producing cells are also present in the thymuses of naive wild-type mice (2, 3). In most mice, IL-17-producing thymocytes represent a subpopulation of CD4 ϩ T cells which are able to react immediately on environmental stimuli without further priming phase (2-5). Furthermore, innate immune cells can also produce IL-17 in response to early immune mechanisms (6). Intrinsically, IL-17-producing cells, also referred to as the sentinels of the immune system, play a critical role in several fields of innate and adaptive immunity, including immunity against microbial infections and tumors (6-9). Dysregulated IL-17 production can result in uncontrolled proinflammatory cytokine production and chronic inflammation, promoting tissue damage and leading to autoimmune diseases (10-12). The most commonly used animal model for the human inflammatory demyelinating disease multiple sclerosis is experimental autoimmune encephalomyelitis (EAE) (13).Systemic bacterial infections and bacterial sepsis are dangerous and potentially lethal disease conditions t...

show abstract

“…Pseudomonas aeruginosa is an opportunistic pathogen known to cause a range of infections, including infections in wounds, intra-abdominal and urogenital sepsis as well as pneumonia in immunocompromised individuals and in children suffering from cystic fibrosis [25,26]. Moreover,…”

Section: Bacteria Causing Food-borne Diseases Such As Staphylococcusmentioning

confidence: 99%

Epidihydropinidine, the main piperidine alkaloid compound of Norway spruce (Picea abies) shows promising antibacterial and anti-Candida activity

et al. 2017

View full text Add to dashboard Cite

ArtikkelitLuonnontieteiden This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T

show abstract

Pneumonia Due to Pseudomonas aeruginosa

Cited by 225 publications

References 125 publications

Role of Iron Uptake Systems in Pseudomonas aeruginosa Virulence and Airway Infection

Role of Iron Uptake Systems in Pseudomonas aeruginosa Virulence and Airway Infection

Pseudomonas aeruginosa and Its Bacterial Components Influence the Cytokine Response in Thymocytes and Splenocytes

Epidihydropinidine, the main piperidine alkaloid compound of Norway spruce (Picea abies) shows promising antibacterial and anti-Candida activity

Contact Info

Product

Resources

About