PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis

Li, Chengcheng; Liu, Shenghao; Yao, Xinghao; Wang, Jing; Wang, Tailin; Zhang, Zhaohui; Zhang, Pengying; Chen, Kaoshan

doi:10.1007/s10725-017-0314-z

Cited by 33 publications

(24 citation statements)

References 40 publications

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“…Flavonoid 3′-monooxygenase is a class of oxidoreductase and is involved in the biosynthesis of flavonoid compounds. PnF3H (encoding a flavanone 3-hydroxylase), isolated from the Antarctic moss Pohlia nutans , improves the salt stress tolerance in transgenic Arabidopsis [79]. Our RT-qPCR analysis showed that the expression trend of these genes basically agrees with the RNA-seq results, with different degrees of inductions under heat treatment.…”

Section: Discussionsupporting

confidence: 66%

Identification of Potential Genes Responsible for Thermotolerance in Wheat under High Temperature Stress

Jiang²,

Qin³

et al. 2019

Genes

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Wheat, a major worldwide staple food crop, is relatively sensitive to a changing environment, including high temperature. The comprehensive mechanism of heat stress response at the molecular level and exploitation of candidate tolerant genes are far from enough. Using transcriptome data, we analyzed the gene expression profiles of wheat under heat stress. A total of 1705 and 17 commonly differential expressed genes (DEGs) were identified in wheat grain and flag leaf, respectively, through transcriptome analysis. Gene Ontology (GO) and pathway enrichment were also applied to illustrate the functions and metabolic pathways of DEGs involved in thermotolerance of wheat grain and flag leaf. Furthermore, our data suggest that there may be a more complex molecular mechanism or tighter regulatory network in flag leaf than in grain under heat stress over time, as less commonly DEGs, more discrete expression profiles of genes (principle component analysis) and less similar pathway response were observed in flag leaf. In addition, we found that transcriptional regulation of zeatin, brassinosteroid and flavonoid biosynthesis pathways may play an important role in wheat’s heat tolerance. The expression changes of some genes were validated using quantitative real-time polymerase chain reaction and three potential genes involved in the flavonoid biosynthesis process were identified.

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Section: Discussionsupporting

confidence: 66%

Identification of Potential Genes Responsible for Thermotolerance in Wheat under High Temperature Stress

Jiang²,

Qin³

et al. 2019

Genes

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“…In plants nonheme iron dioxygenases take part in the biosynthesis of a series of flavonoids, which are important plant hormones that trigger fruit colouring [16,17], fruit ripening [18] but also operate as stress responses [19,20]. Furthermore, these compounds have been shown to have health benefits in humans and have been correlated to antioxidant and anticancer properties and blood pressure control [21].…”

Section: Introductionmentioning

confidence: 99%

Flavonol biosynthesis by nonheme iron dioxygenases: A computational study into the structure and mechanism

Zeb

Rashid

Mubarak

et al. 2019

Journal of Inorganic Biochemistry

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Plants produce flavonol compounds for vital functions regarding plant growth, fruit and flower colouring as well as fruit ripening processes. Several of these biosynthesis steps are stereo-and regioselective and are being carried out by nonheme iron enzymes. Using density functional theory calculations on a large active site model complex of flavanone-3hydroxylase (FHT), we established the mechanism for conversion of naringenin to its dihydroflavonol, which is a key step in the mechanism of flavonol biosynthesis. The reaction starts with dioxygen binding to the iron(II) centre and a reaction with -ketoglutarate cosubstrate gives succinate, an iron(IV)-oxo species and CO 2 with large exothermicity and small reaction barriers. The rate-determining reaction step in the mechanism; however, is hydrogen atom abstraction of an aliphatic C-H bond by the iron(IV)-oxo species. We identify a large kinetic isotope effect for the replacement of the transferring hydrogen atom by deuterium. In a final step the OH and substrate radicals combine to form the alcohol product with a barrier of several kcal mol-1. We show that the latter is the result of geometric constraints in the active site pocket. Furthermore, the calculations show that a weak tertiary C-H bond is shielded from the iron(IV)-oxo species in the substrate binding position and therefore the enzyme is able to activate a stronger C-H bond. As such, the flavanone-3hydroxylase enzyme reacts regioselectively with one specific C-H bond of naringenin by avoiding activation of weaker bonds through tight substrate and oxidant positioning.

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“…A previous study also showed that F3H plays an important role in plant biotic and abiotic stresses [34]. Overexpression of F3H gene can improve drought resistance in tobacco and increase salt tolerance in Arabidopsis [35,36]. F3H also catalyzes the formation of dihydro avonol (DHK) in the avonoid pathway, which interacts synergistically with different genes in a variety of species [31].…”

Section: Discussionmentioning

confidence: 99%

Mapping and identification of yellow seed coat color genes in Brassica juncea L.

Huang

Wang

Lü³

et al. 2020

Preprint

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BackgroundYellow seed breeding is an effective method to improve the oil content in rapeseed. Yellow seed coat color formation is influenced by various factors, and no clear mechanisms are known. In this study, Bulked segregant RNA-Seq (BSR-Seq) of BC9 population of Wuqi mustard (yellow seed) and Wugong mustard (brown seed) was used to identity the candidate genes controlling the yellow seed color in Brassica juncea L.ResultsYellow seed coat color gene was mapped to chromosome A09, and differentially expressed genes (DEGs) between brown and yellow bulks enriched in the flavonoid pathway. A significant correlation between the expression of BjF3H and BjTT5 and the content of the seed coat color related indexes was identified. Two intron polymorphism (IP) markers linked to the target gene were developed around BjF3H. Therefore, BjF3H was considered as the candidate gene. The BjF3H coding sequences (CDS) of Wuqi mustard and Wugong mustard are 1071-1077bp, encoding protein of 356-358 amino acids. One amino acid change (254, F/V) was identified in the conserved domain. This mutation site was detected in four Brassica rapa (B. rapa) and six Brassica juncea (B. juncea) lines, but not in Brassica napus (B. napus).ConclusionsThe results indicated BjF3H is a candidate gene that related to yellow seed coat color formation in Brassica juncea and provided a comprehensive understanding of the yellow seed coat color mechanism.

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PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis

Cited by 33 publications

References 40 publications

Identification of Potential Genes Responsible for Thermotolerance in Wheat under High Temperature Stress

Identification of Potential Genes Responsible for Thermotolerance in Wheat under High Temperature Stress

Flavonol biosynthesis by nonheme iron dioxygenases: A computational study into the structure and mechanism

Mapping and identification of yellow seed coat color genes in Brassica juncea L.

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