PNPase and RhlB Interact and Reduce the Cellular Availability of Oxidized RNA in Deinococcus radiodurans

Abstract

Oxidative RNA damage can be caused by oxidative stress, such as hydrogen peroxide, ionizing radiation, and antibiotic treatment. 8-oxo-7,8-dihydroguanine (8-oxoG), a major type of oxidized RNA, is highly mutagenic and participates in a variety of disease occurrences and development. Although several proteins have been identified to recognize 8-oxoG-modified RNA, the knowledge of how RNA oxidative damage is controlled largely remains unclear, especially in nonmodel organisms.

Unraveling radiation resistance strategies in two bacterial strains from the high background radiation area of Chavara-Neendakara: A comprehensive whole genome analysis

“…D . radiodurans is reported to have pnp gene, important in removing 8-oxo-ribonucleotide, formed after IR exposure and may also interact with other DNA repair genes [ 194 ].…”

Memory Effect on the Survival of Deinococcus radiodurans after Exposure in Near Space

“…There is documented evidence that cell death strongly correlates with proteome carbonylation (PC) caused by ROS ( 50 , 51 , 71 , 72 ). The presence of a biological response to cellular damage or protection against molecular damage, such as that by ROS, ensures the survival of bacteria exposed to radiation ( 71 , 75 ). According to published literature, numerous antioxidant proteins, e.g., superoxide dismutase (SOD), OxyR, PerR, DdrI, and PprI, as well as various antioxidant metabolites against ROS and PC, help to enhance protection of the proteome of D. radiodurans ( 50 ).…”

“…Moreover, some studies showed that many small RNAs also play essential roles in the transport of antioxidant proteins and regulation of manganese and DNA repair systems in D. radiodurans ( 73 , 74 ). Some proteins reduced the effect of oxidized RNA, which also helped protect cells from oxidative stress ( 75 ). Growing in stress environments may upgrade these factors, which help survival in exposure.…”

DNA repair and oxidative stress defense systems in radiation-resistant Deinococcus murrayi

“…Identified non-enzymatic antioxidant systems and compounds are a high intracellular Mn 2+ /Fe 2+ ratio (limiting Fe 2+ -catalyzed oxidative damage to proteins), small-molecule Mn 2+ -antioxidant complexes, small peptides, carotenoids, and the low-molecular-weight thiol bacillithiol (Daly et al 2004(Daly et al , 2010Tian and Hua 2010;Berlett and Levine 2014;Jeong et al 2021a). Antioxidant enzymes are also important for survival of Deinococcus when exposed to radiation (acute or chronic), desiccation, or oxidants (Lim et al 2019;de Groot et al 2022;Gaidamakova et al 2022;Han et al 2022) (and references therein). These enzymes are for example involved in ROS removal (e.g., superoxide dismutase, catalase, and peroxiredoxin), and protection and repair of oxidation-sensitive cysteine and methionine residues in proteins (thiol reductases).…”