PO-0385a Adverse Childhood Experiences In Alberta, Canada: A Population Based Study

Abstract

anti-T. gondii IgG in oral fluid. It was then applied to 150 children aged 0-15 months (341 samples) born from 133 women who seroconverted during pregnancy and 17 who remained seronegative. IgG on oral fluid were compared to serum IgG detected with MEIA AxSYM ® Toxo IgG (Abbott Laboratories). Results The pilot study validated the acceptability and the safety of the test and the adequate duration of sampling. IgG detected in serum and in oral fluid had a parallel kinetics among newborns (correlation coefficient: 0.59, p < 0.0001), with a concordant decline in the non-infected ones (n = 110), and matching raising or stable IgG in those who were congenitally-infected (n = 23). Conclusions Collection of oral fluid is painless and inexpensive.Our new test provides a simple and rapid method to detect antiToxoplasma gondii IgG and to manage newborn at risk for congenital infection. It could have many other applications in pregnant women and other groups of patients. Glycogen storage diseases (GSD) are a group of inherited disorders of metabolism that result in storage of excess glycogen. Several well defined defects in one of the enzymes involved in the synthesis or degradation of glycogen have been described. There are over 15 types and they are classified based on the enzyme deficiency and the affected tissue (liver, muscle or both). PO-0384 THE PRACTICAL METHOD TO DIAGNOSIS OF FOURTEEN CASES OF GLYCOGEN STORAGE DISEASES IN OUR LABORATORYIn this study, we wish to report the biochemical investigations adopted in main infantile GSD diagnosed in our laboratory.Four steps diagnostic procedure have been assumed, taking into account several frequent clinical observations leading to further targeted biochemical parameters:1. Assessment of the metabolic disorders with standard tests (fast blood glucose, uric acid, triglycerides, total cholesterol, ASAT, ALAT, CK, lactic acid).2. Quantitative determination of glycogen in leucocytes (or erythrocytes) after extraction, precipitation and treatment with an throne reagent.3. Oral galactose test with blood lactate and glucose estimations, in combination with a glucagon tolerance test to screen the main types of liver glycogenosis.4. Lysosomal acid a-glucosidase activity when GSD type II (Pompe disease) is suspected.Since 1995 and on the basis of this screening procedure and clinical features, 14 cases of GSD have been categorised: · 6 forbes's disease (GSD III, debranching-enzyme deficiency) · 3 von Gierke's disease (GSD I, glucose-6-phosphatase deficiency) · 2 pompe's disease (GSD II, maltase acid deficiency) · 1 Andersen's disease (GSD IV, branching-enzyme deficiency) · 1 Hers's disease (GSD VI, hepatic phosphorylase deficiency) · 1 GSD IX (phosphorylase kinase deficiency) Our laboratory diagnostic approach include simple screening tests easy to implement in clinical chemistry laboratories. Thus, Pompe disease diagnosis is easily done in our laboratory. The measurement of tissue enzyme activities (liver and muscle) of the other enzymes is limited to some specialised laboratories....

Childhood adversities and rate of adulthood all-cause hospitalization in the general population: A retrospective cohort study

“…Extensive literature suggests that childhood adversities affect one's physiological and psychological development, leading to adverse health outcomes. Childhood adversities have been reported to be strongly associated with psychosocial adversities such as poor social engagement, unstable relationships; health risk behaviors such as smoking and substance use; chronic conditions such as depression, distress, and cancer; socioeconomic deprivation such as poor income and education, and even premature mortality in adulthood [2,[6][7][8]. These associations of childhood adversities with adverse physical, mental, and social health suggest that through these potentially mediating mechanisms, there may be an increased need for, and utilization of, health care services in adulthood among people reporting childhood adversities.…”