PO-0399 Neurodevelopmental And Behaviour Outcome Of Preterms (ga

Abstract

Background Hypoxic-ischaemic encephalopathy leads to neurologic impairment or even death. Secretoneurin (SN), a neuropeptide with angiogenic and anti-apoptotic properties, provided strong neuroprotection in an adult animal model of cerebral ischaemia. Aim To evaluate the effect of SN in established in vivo and in vitro models of neonatal hypoxic-ischaemic brain injury. Methods Seven day old mice underwent unilateral common carotid artery ligation, followed by exposure to hypoxia (8% oxygen). Thereafter, mouse pups were randomly injected intraperitoneally with SN (0.25 mg/g body weight) or vehicle. As endpoint we determined the histological injury score and the number of caspase-3 positive cells 24 h after the insult. Primary cultured hippocampal neurons were treated with oxygen glucose deprivation (OGD) on day 10. Neurons were assigned to the following groups: i) control ii) OGD iii) OGD+SN (1, 10 or 50 mg/l). As primary outcome parameter, cell death was evaluated via real time live confocal imaging using calcein-AM and propidium iodide (PI). Results SN displayed a non-significant trend to lower mean values of histological injury score compared to control (n = 11-12, p > 0.05) and significantly reduced the number of cells stained positively for activated caspase-3 (n = 6, p < 0.05). In vitro SN application on hippocampal neurons (OGD+SN) significantly reduced the number of dead cells assessed by the PI/calcein ratio compared with the untreated OGD group (n = 8, p < 0.05). Conclusion We provide first evidence that SN is neuroprotective in established in vitro and in vivo models of neonatal hypoxicischaemic brain injury and might therefore be considered a promising therapeutic option. Background Previous studies in 10-year-old children have shown an association between size of the corpus callosum and neurodevelopmental outcome. Aim In the present study we examined whether diffusion changes in the corpus callosum shortly after birth are associated with outcome in full-term infants with perinatal asphyxia. Subjects/methods From 2002 to 2013, 127 full-term infants with perinatal asphyxia were examined using diffusion weighted MRI within 7 days after birth (median 4 days; IQR 2 days). To calculate ADC values, regions of interest were manually selected in the anterior and posterior part of the corpus callosum, and ADC values were related to outcome. Adverse outcome was defined as death (n = 33), cerebral palsy or delayed development at 18 moths (Griffiths DQ <85): n = 12. Sixtynine of the infants (54.3%) received therapeutic hypothermia. Background and aims We aimed to identify the neurodevelopmental and behaviour outcome of preterms (GA <30 wks) at 8 years by age appropriate psychometric evaluations to see whether tests used at younger ages could predict worst outcomes at older ages in relation to some neonatal factors. Method Along with neurologic examinations, 33 infants were prospectively evaluated at 3, 6, 12,18, 24 months of corrected age with Bayley Scales of Infant Development -II (BSID-II), at 3, 5 years with ...