Pod Mildew on Soybeans Can Mitigate the Damage to the Seed Arising from Field Mold at Harvest Time

Liu, Jiang; Deng, Juzhi; Zhang, Ke; Wu, HengAn; Yang, Caiqiong; Zhang, Xiaowen; Du, Junbo; Shu, Kai; Yang, Wenyu

doi:10.1021/acs.jafc.6b03561

Cited by 14 publications

(19 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our recent studies on FM in preharvest soybean seed, we found that mildew resistance was significantly correlated with the seed coat color and that soybean seeds with dark coats showed higher field mold resistance [ 13 ]. Although the obtained evidence confirmed that the antimycotic constituent primarily originated from the medium-polarity fraction [ 14 ], we believe that new functional constituents, especially polysaccharides, are contained in the wasted high-polarity, water-soluble fraction of the black soybean seed coat. In the present study, a three-level, three-factor Box-Behnken design was combined with response surface methodology (RSM) to optimize the extraction conditions for obtaining the maximum yield of BSCPs.…”

Section: Introductionmentioning

confidence: 76%

Extraction optimization, purification and characterization of polysaccharides from the seed coat of black soybean

Hu¹,

Deng²,

Yang

et al. 2017

PLoS ONE

Self Cite

View full text Add to dashboard Cite

In this study, the extraction of water-soluble polysaccharides from the seed coat of black soybean (BSCP) was investigated and optimized. A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction temperature, 100°C; ratio of water to material, 22.3 mL/g; and extraction time, 133.2 min. Under these conditions, the experimental yield of polysaccharides was 10.56%, which was consistent with the predictive yield. A novel galactomannan, BSCP-1, with a molecular weight of 7.55 × 105 Da determined by high-performance gel permeation chromatography, was isolated from the black soybean seed coat. Through gas chromatography-mass spectrometry analysis, BSCP-1 was identified as a galactomannan consisting of galactose, mannose and rhamnose in a molar ratio of 6.01:3.56:1.00. Cytotoxicity against the human gastric carcinoma cancer cell line was also determined.

show abstract

Section: Introductionmentioning

confidence: 76%

Extraction optimization, purification and characterization of polysaccharides from the seed coat of black soybean

Hu¹,

Deng²,

Yang

et al. 2017

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nowadays, various analytical platforms were widely used in the study of soybean metabolism and corresponding molecular mechanism elucidation (Chai et al, 2012;Lee et al, 2017;Silvente, Sobolev, & Lara, 2012;Sun, Feng, & Ort, 2014;Wu et al, 2008). However, metabolomics researches on soybean mainly focus on seed and leaf rather than pod, which was demonstrated as an important organ in the FM resistance (Liu et al, 2016). Single solvent is widely used in different plant tissue and organs (Benkeblia, Shinano, & Osaki, 2007;Chebrolu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Development and validation of a GC–MS method for soybean organ-specific metabolomics

Hu¹,

Yang

Iqbal³

et al. 2018

Plant Production Science

Self Cite

View full text Add to dashboard Cite

Field mold (FM) can easily deteriorate the preharvest soybean in the field, and Fusarium moniliforme is demonstrated as the dominant pathogenic fungi. Metabolomics is a powerful tool to reveal the resistance mechanism in response to microbial infection. Therefore, in this research, the Design of Experiment (DOE) model was developed to optimize the extraction solvent combinations for metabolomic study of soybean seed and pod based on gas chromatographymass spectrometry (GC-MS). Combined with the number of extracted peaks and the peak area of common substances, the extraction efficiency of different solvent was analyzed by multivariate statistical analysis. The result showed that isopropanol/water/methanol (1:1:1 and 1:1:4, v/v/v) mixture was highly efficient for metabolites extractions of soybean seed and pod, respectively. Additionally, the potential metabolites and pathways concerned in FM resistance were explored by the optimized extraction solvent system based on metabolomics analysis. Amino acid metabolism in soybean seed was disturbed by F. moniliforme and metabolic pathways related to energy conversion in soybean pod strongly responded to fungal infection. This study constructs a GC-MS-based metabonomic method for soybean metabolites; comparative analysis of organspecific metabolomics for soybean fruit could be further applied in soybean metabolomics researches.

show abstract

“…Grain fungi can be divided into two large categories, field and storage molds, based on the location of the outbreak (Liu et al, 2016). Storage fungi can grow at low water activity, which occurs readily under a high-temperature (25–30°C) and humid environment.…”

Section: Discussionmentioning

confidence: 99%

“…Our recent research demonstrated a potentially important function of soybean pods in FM resistance at the phytochemical level (Liu et al, 2016). With increases in the contents of infection components, such as proteins, carbohydrates, and fatty acids, the MIP increased and the MIS decreased, demonstrating indirectly that pod mildew can help mitigate the corresponding seed mildew to a certain extent.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fungal Diversity in Field Mold-Damaged Soybean Fruits and Pathogenicity Identification Based on High-Throughput rDNA Sequencing

Liu

Jun²,

Cai

et al. 2017

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Continuous rain and an abnormally wet climate during harvest can easily lead to soybean plants being damaged by field mold (FM), which can reduce seed yield and quality. However, to date, the underlying pathogen and its resistance mechanism have remained unclear. The objective of the present study was to investigate the fungal diversity of various soybean varieties and to identify and confirm the FM pathogenic fungi. A total of 62,382 fungal ITS1 sequences clustered into 164 operational taxonomic units (OTUs) with 97% sequence similarity; 69 taxa were recovered from the samples by internal transcribed spacer (ITS) region sequencing. The fungal community compositions differed among the tested soybeans, with 42 OTUs being amplified from all varieties. The quadratic relationships between fungal diversity and organ-specific mildew indexes were analyzed, confirming that mildew on soybean pods can mitigate FM damage to the seeds. In addition, four potentially pathogenic fungi were isolated from FM-damaged soybean fruits; morphological and molecular identification confirmed these fungi as Aspergillus flavus, A. niger, Fusarium moniliforme, and Penicillium chrysogenum. Further re-inoculation experiments demonstrated that F. moniliforme is dominant among these FM pathogenic fungi. These results lay the foundation for future studies on mitigating or preventing FM damage to soybean.

show abstract

Pod Mildew on Soybeans Can Mitigate the Damage to the Seed Arising from Field Mold at Harvest Time

Cited by 14 publications

References 19 publications

Extraction optimization, purification and characterization of polysaccharides from the seed coat of black soybean

Extraction optimization, purification and characterization of polysaccharides from the seed coat of black soybean

Development and validation of a GC–MS method for soybean organ-specific metabolomics

Fungal Diversity in Field Mold-Damaged Soybean Fruits and Pathogenicity Identification Based on High-Throughput rDNA Sequencing

Contact Info

Product

Resources

About