POF Biosensors Based on Refractive Index and Immunocapture Effect

Werneck, Marcelo Martins; Lopes, Rafaela N.; Costa, G.; Rodrigues, Domingos M. C.; Arcas, Ariadny da Silva; Dutra, Fabio da Silva; Queiroz, Vanessa M.; Allil, Regina C.

doi:10.1007/978-3-319-42625-9_4

Cited by 3 publications

(5 citation statements)

References 19 publications

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“…It can be clearly seen from plots on the Figure 7 that bare U-shaped probes showed a linear relationship between sensor output voltage and sucrose solutions RI for the whole range, indirectly proportional to RI changes, which agreed with the results of [ 19 , 23 ]. The R 2 coefficients were greater than 0.99 for all sensors, which demonstrate a good fit of the linear equations with experimental data.…”

Section: Resultssupporting

confidence: 87%

“…At the same time, the output voltage of reference sensor remained constant, which confirmed the proper operation of the sensor for bacteria detection. As shown in previous works [ 19 , 23 ], U-shaped probes based on bending loss effect had an indirect relationship between sensor response and RI. Nonetheless, in Figure 9 b, the sensor output voltage increased in time as more and more bacteria were bound to sensor surface.…”

Section: Resultssupporting

confidence: 53%

“…The technique known as immunocapture consists in covalently bonding the specific antibody on the sensor sensitive region in such a way that only bacteria of specific species are captured and fixed on the sensor surface [ 19 , 23 , 24 ].…”

Section: Working Principlementioning

confidence: 99%

“…The attenuation phenomenon by the fiber bend is known as bending loss. An illustration of the bare U-shaped POF probe operating by bending loss effect in the presence of bacteria is showed in Figure 1 [ 23 , 31 , 32 , 33 , 34 ].…”

Section: Working Principlementioning

confidence: 99%

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Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors

Arcas

Dutra

Allil

et al. 2018

Sensors

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Escherichia coli (E. coli) is a large and diverse bacteria group that inhabits the intestinal tract of many mammals. Most E. coli strains are harmless, however some of them are pathogenic, meaning they can make one sick if ingested. By being in the feces of animals and humans, its presence in water and food is used as indicator of fecal contamination. The main method for this microorganism detection is the bacterial culture medium that is time-consuming and requires a laboratory with specialized personnel. Other sophisticated methods are still not fast enough because they require sending samples to a laboratory and with a high cost of analysis. In this paper, a gold-coated U-shaped plastic optical fiber (POF) biosensor for E. coli bacteria detection is presented. The biosensor works by intensity modulation principle excited by monochromatic light where the power absorption is imposed by predominant effect of either bending loss or surface plasmon resonance (SPR), depending on the gold thickness. Bacterial selectivity is obtained by antibody immobilization on the fiber surface. The biosensor showed a detection limit of 1.5 × 103 colony-forming units (CFU)/mL, demonstrating that the technology can be a portable, fast response and low-cost alternative to conventional methodologies for quality analysis of water and food.

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Section: Resultssupporting

confidence: 87%

Section: Resultssupporting

confidence: 53%

Section: Working Principlementioning

confidence: 99%

Section: Working Principlementioning

confidence: 99%

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Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors

Arcas

Dutra

Allil

et al. 2018

Sensors

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“…The output signal from the transimpedance amplifier is acquired into an Arduino Microcontroller connected to a computer by an USB cable. The output voltage, proportional to the optical power, is read and shown in the computer display, as detailed in [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results

Rodrigues

Lopes

Franco³

et al. 2017

Sensors

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Conventional pathogen detection methods require trained personnel, specialized laboratories and can take days to provide a result. Thus, portable biosensors with rapid detection response are vital for the current needs for in-loco quality assays. In this work the authors analyze the characteristics of an immunosensor based on the evanescent field in plastic optical fibers with macro curvature by comparing experimental with simulated results. The work studies different shapes of evanescent-wave based fiber optic sensors, adopting a computational modeling to evaluate the probes with the best sensitivity. The simulation showed that for a U-Shaped sensor, the best results can be achieved with a sensor of 980 µm diameter by 5.0 mm in curvature for refractive index sensing, whereas the meander-shaped sensor with 250 μm in diameter with radius of curvature of 1.5 mm, showed better sensitivity for either bacteria and refractive index (RI) sensing. Then, an immunosensor was developed, firstly to measure refractive index and after that, functionalized to detect Escherichia coli. Based on the results with the simulation, we conducted studies with a real sensor for RI measurements and for Escherichia coli detection aiming to establish the best diameter and curvature radius in order to obtain an optimized sensor. On comparing the experimental results with predictions made from the modelling, good agreements were obtained. The simulations performed allowed the evaluation of new geometric configurations of biosensors that can be easily constructed and that promise improved sensitivity.

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Developing highly efficient and electric-field-sensitive fiber-waveguide evanescent couplers

Cheng

Liu

et al. 2021

Appl. Opt.

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The slab-coupled optical fiber sensor (SCOS) is an innovative electric field detector with an ultrawide measuring range and a millimeter-level package size. The core sensing part of the SCOS is a fiber-waveguide evanescent coupler (FWEC) that directly determines the device’s main performance specifications. This paper presents an investigation of the spectrum characteristics of FWECs with various structural and curing parameters. Methods for fabricating an FWEC with higher resonant depth, narrower free spectral range, and sharper spectrum slope are determined based on the experimental results. Z-cut lithium-niobate FWEC and Z-cut lithium-tantalate FWEC of about 1 × 0.5 × 0.3 m m 3 are fabricated on this basis. Excellent coupling characteristics are achieved in both, according to the relevant spectra. Electric field tests indicate that the peak wavelengths shift linearly with the external AC field amplitude by 0.11 nm/(kV/cm) and 0.24 nm/(kV/cm), respectively. Three optimization methods are proposed to enhance performance: optimizing material selection, adding an antenna structure, and adjusting the calibration method. The results of this work may provide workable guidance for developing miniature, all-dielectric electric field sensors.

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POF Biosensors Based on Refractive Index and Immunocapture Effect

Cited by 3 publications

References 19 publications

Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors

Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors

Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results

Developing highly efficient and electric-field-sensitive fiber-waveguide evanescent couplers

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