2000
DOI: 10.1021/bi0011274
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Point Mutations within AT-Hook Domains of the HMGI Homologue HMGIYL1 Affect Binding to Gene Promoter but Not to Four-Way Junction DNA

Abstract: High-mobility group I/Y (HMGI/Y) proteins are chromosomal proteins involved in gene and chromatin regulation. Elevated levels of HMGI/Y proteins were reported in diverse malignant tumors, and rearrangements of their genes are casually involved in the development of benign tumors. In humans, the chromosomal locus Xp22 has been often found to be affected in diverse benign mesenchymal tumors. Recent studies revealed that this region contains a retropseudogene HMGIYL1 which potentially can be activated in a way of… Show more

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Cited by 8 publications
(3 citation statements)
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“…In vitro studies demonstrated that several kinases, including CDC2, PKC, casein kinase 2, and HIPK2, modify distinct residues in HMGA proteins, and that these modifications alter the binding of the proteins to their targets (Schwanbeck et al 2000; Reeves and Beckerbauer 2001; Zhang and Wang 2007). During mitosis, PKC and CDC2 hyperphosphorylate Ser and Thr residues next to each AT hook, suggesting that some of the modifications are cell-cycle related (Nissen et al 1991).…”
Section: Hmga Proteinsmentioning
confidence: 99%
“…In vitro studies demonstrated that several kinases, including CDC2, PKC, casein kinase 2, and HIPK2, modify distinct residues in HMGA proteins, and that these modifications alter the binding of the proteins to their targets (Schwanbeck et al 2000; Reeves and Beckerbauer 2001; Zhang and Wang 2007). During mitosis, PKC and CDC2 hyperphosphorylate Ser and Thr residues next to each AT hook, suggesting that some of the modifications are cell-cycle related (Nissen et al 1991).…”
Section: Hmga Proteinsmentioning
confidence: 99%
“…1, 2A, and 5A) (31-33). INF-␤, which contains PRDIII-1, PRDII, and NRDI enhancer elements, was chosen as a probe because it is bound by HMGA AT-hooks in vivo and in vitro (26,(31)(32)(33)(34)(35)(36)(37). TAF1-2 and TAF1-4, the isoforms that contain two AT-hooks, altered the mobility of the INF-␤ probe, indicating that they bind INF-␤ DNA (Fig.…”
Section: Taf1 Alternative Exon 12a Encodes An At-hook Motif-mentioning
confidence: 99%
“…However, a thorough study of such pseudogenic proteins has been impeded and delayed by the absence of antibodies that do not recognize their full-length, functional counterparts. Pseudogenic open reading frames (ORFs) have been either translated in bacteria or overexpressed as tagged or fusion proteins (35)(36)(37)(38)(39)(40)(41), methods that limit the study of their expression, subcellular localization, and function. Unbiased and high-throughput techniques should enable the acquisition of a comprehensive catalog of pseudogenic proteins.…”
Section: Duplication and Mutationmentioning
confidence: 99%