Point-of-Care Determination of Acetaminophen Levels with Multi-Hydrogen Bond Manipulated Single-Molecule Recognition (eMuHSiR)

Abstract

This work aims to face the challenge of monitoring small molecule drugs accurately and rapidly for point-of-care (POC) diagnosis in current clinical settings. Overdose of acetaminophen (AP), a commonly used over the counter (OTC) analgesic drug, has been determined to be a major cause of acute liver failure in the US and the UK. However, there is no rapid and accurate detection method available for this drug in the emergency room. The present study examined an AP sensing strategy that relies on a previously unexplored strong interaction between AP and the arginine (Arg) molecule. It was found that as many as 4 hydrogen bonds can be formed between one Arg molecule and one AP molecule. By taking advantages of this structural selectivity and high tenability of hydrogen bonds, Arg, immobilized on a graphene surface via electrostatic interactions, was utilized to structurally capture AP. Interestingly, bonded AP still remained the perfect electrochemical activities. The extent of Arg-AP bonds was quantified using a newly designed electrochemical (EC) sensor. To verify the feasibility of this novel assay, based on multihydrogen bond manipulated single-molecule recognition (eMuHSiR), both pharmaceutical and serum sample were examined. In commercial tablet measurement, no significant difference was seen between the results of eMuHSiR and other standard methods. For measuring AP concentration in the mice blood, the substances in serum, such as sugars and fats, would not bring any interference to the eMuHSiR in a wide concentration range. This eMuHSiR method opens the way for future development of small molecule detection for the POC testing.

Electrogenerated chemiluminescence of a Ru(bpy)32+/arginine system: a specific and sensitive detection of acetaminophen

“…As a proof of concept, we apply the Ru(bpy) 3 2+ /Arg system for Ace detection. As reported previously, 16,30 there is a specic interaction between Arg and Ace based on the formation of as many as four hydrogen bonds. As shown in Fig.…”

“…The hydroxyl group of Ace could interact with the amino group of Arg to form hydrogen bonds. 30 The greater number of hydrogen bonds formed attributes to the lower absorbance of hydroxyl group at 199 nm. The maximum number of hydrogen bonds are formed when the ratio of Arg : AP is 50 : 50 ( Fig.…”

Fast and Facile Voltammetric Detection of Acetaminophen at Poly (DL-phenylalanine) Modified Dysprosium-Copper Oxide Nanoparticle/Carbon Composite Paste Electrode

“…As can be noted from the Nyquist diagram shown in Fig. (5), the largest semicircle at the high frequency was observed for bare electrode with an R ct value of 9145 Ω. After modifying with polymer, the R ct value was found to minimise to a value of 206 Ω.…”

Analisis Molekular dan Transpor Ion Amonium Clorida

“…Banyak senyawa yang paling penting pada bidang industry, seperti amonia (42)(43)(44)(45)(46) , asam nitrat (47) , nitrat organik (propelan dan bahan peledak), serta sianida, mengandung nitrogen. Nitrogen banyak ditemukan dalam bentuk ikatan rangkap dua dan tiga, namun ada juga yang membentuk ikatan tunggal(single bond) (48)(49)(50)(51)(52)(53) . Ikatan rangkap tiga pada atom nitrogen sangat kuat jika dibandingkan dengan ikatan tunggal dan ikatan rangkap dua.…”