Point-of-Care Diagnoses and Assays Based on Lateral Flow Test

Pohanka, Miroslav

doi:10.1155/2021/6685619

Cited by 26 publications

(14 citation statements)

References 83 publications

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“…The majority of Ag-RDTs are equipped with a colorimetric readout [ 22 , 23 ]. In comparison, electrochemical detection methods are inherently more sensitive since they rely on direct transduction of electrons.…”

Section: Introductionmentioning

confidence: 99%

Rapid electrochemical immunodetection of SARS-CoV-2 using a pseudo-typed vesicular stomatitis virus model

Ashur

Alter

Werbner

et al. 2022

Talanta

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The COVID-19 pandemic has highlighted the need for reliable and accurate diagnostic tools that provide quantitative results at the point of care. Real-time RT-PCR requires large laboratories, a skilled workforce, complex and costly equipment, and labor-intensive sample processing. Despite tremendous efforts, scaling up RT-PCR tests is seemingly unattainable. To date, hundreds of millions of COVID-19 tests have been performed globally, but the demand for timely, accurate testing continues to outstrip supply. Antigen-based rapid diagnostic testing is emerging as an alternative to RT-PCR. However, the performance of these tests, namely their sensitivity, is still inadequate. To overcome the limitations of currently employed diagnostic tests, new tools that are both sensitive and scalable are urgently needed. We have developed a miniaturized electrochemical biosensor based on the integration of specific monoclonal antibodies with a biochip and a measurement platform, and applied it in the detection of Spike S1 protein, the binding protein of SARS-CoV-2. Using electrochemical impedance spectroscopy, quantitative detection of sub-nanomolar concentrations of Spike S1 was demonstrated, exhibiting a broad detection range. To demonstrate the applicability of the biosensor, we have further developed a SARS-CoV-2 pseudovirus based on Spike protein-pseudo-typed VSV platform. Specific detection of different concentrations of pseudovirus particles was feasible in <30 min. This new tool may largely contribute to the fight against COVID-19 by enabling intensive testing to be performed and alleviating most of the hurdles that plague current diagnostics.

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Section: Introductionmentioning

confidence: 99%

Rapid electrochemical immunodetection of SARS-CoV-2 using a pseudo-typed vesicular stomatitis virus model

Ashur

Alter

Werbner

et al. 2022

Talanta

View full text Add to dashboard Cite

show abstract

“…In recent years, there has been an increasing demand for point-of-care (POC) multiple diagnostic assays that allow rapid detection of analytes present in samples compared to conventional lab-based testing. A wide range of biosensors based on lateral flow immunoassay (LFIA) is employed in the healthcare sector, environmental sector, and food industries [ 1 , 2 , 3 ]. LFIA is based on affinity interactions such as hybridization of aptamer-target, antibody-antigen, biotin-streptavidin, or probe DNA-target DNA hybridization and offers advantages over conventional analytical methods in terms of low cost, on-site response, ease of operation, portability, robustness, and fast and naked-eye detection [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Optimizing Effective Parameters to Enhance the Sensitivity of Vertical Flow Assay for Detection of Escherichia coli

Kaur

Eltzov

2022

Biosensors

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Vertical flow immunoassays (VFIAs) are considered potential point-of-care testing (POCT) devices compared to lateral flow assays due to their ability to analyze a comparatively large sample volume and ease of multiplexing. However, VFIA devices are limited by low analytical sensitivity when coupled with a visual colorimetric signal. Herein, we carefully analyzed key parameters that accounted for the proper functionality of VFIA that can be modified to enhance the overall sensitivity of VFIA. In particular, we focused on improving the stability of conjugate pads impregnated with capture antibodies, maintaining a controlled flow rate to ensure higher analyte reactivity with capture antibodies, and enhancing the absorption efficiency. The results showed that air-drying of conjugate pads in the presence of 5% (w/v) lactose significantly improved the stability of antibodies during long-term storage. Integration of dissolvable polyvinyl alcohol (PVA) membrane of optimal concentration as a time-barrier film into the sensor delayed the flow of samples, thereby increasing the biorecognition interaction time between immunoreagents for the formation of immuno-complexes, which in turn led to higher sensitivity of the assay. Furthermore, the employment of an absorbent pad with higher water holding capacity significantly reduced the non-specific binding of immunocomplexes, thereby reducing the possibility of false-negative results.

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“…The lateral fl ow tests are also well suited for the point-of-care conditions. The currently available lateral fl ow tests are a simple analytical tool based on the migration of sample through a thin layer soaked with labelled antibodies or antigens and containing two zones with immobilized antibodies or antigens providing typical coloured lines (67)(68)(69)(70). The principle can be described as follows.…”

Section: The Common Antigen Tests and Comparison With The Other Methodsmentioning

confidence: 99%

Point-of-care diagnosis of COVID-19 disease based on antigen tests

Pohanka¹

2021

BLL

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AIMS: This review is focused on the laboratory diagnoses of the coronavirus disease 2019 (COVID-19) by recognizing the antigen of the causative agent SARS-CoV-2 virus. Various antigen tests are available in this moment and these tests are being further developed in order to reach a better diagnostic value. The issue is reviewed in a complex view. METHODS: In this work, a complex survey of the current literature was made. The relevant and recent papers related to antigen tests of COVID-19 are discussed and cited. Basic specifi cations of the antigen tests and competitive methods were also scrutinized in the current literature. RESULTS: The survey of the current literature (years 2019 -2021) was made and diagnostic methods like lateral fl ow tests (lateral fl ow immunochromatographic assay) and various types of biosensors were specifi ed as tools for COVID-19 diagnosis and their application to be used as a point-of-care test is considered. CONCLUSIONS: Small hand-held assays applicable in the point-of-care conditions for diagnosis of COVID-19 by analysis of SARS-CoV-2 antigen are the means of a growing interest and these means undergo a signifi cant development leading to the improvements of their specifi cations and applicability to the current praxis. Merit of the assays is discussed in this paper (Tab. 3, Fig. 2, Ref. 109).

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Point-of-Care Diagnoses and Assays Based on Lateral Flow Test

Cited by 26 publications

References 83 publications

Rapid electrochemical immunodetection of SARS-CoV-2 using a pseudo-typed vesicular stomatitis virus model

Rapid electrochemical immunodetection of SARS-CoV-2 using a pseudo-typed vesicular stomatitis virus model

Optimizing Effective Parameters to Enhance the Sensitivity of Vertical Flow Assay for Detection of Escherichia coli

Point-of-care diagnosis of COVID-19 disease based on antigen tests

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