Point-of-care diagnostics for therapeutic monitoring of levofloxacin in human plasma utilizing electrochemical sensor mussel-inspired molecularly imprinted copolymer

Azab, Noha F. El; Mahmoud, Amr M.; Trabik, Yossra A.

doi:10.1016/j.jelechem.2022.116504

Cited by 32 publications

(14 citation statements)

References 71 publications

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“…Many factors can influence the precision of the SC-ISE, especially temperature and pH among other experimental parameters such as weighing and pipetting. But the high precision reported in our work and similar ion-selective electrodes reported in the literature [38][39][40] can be attributed to the logarithmic nature of potentiometric response with changing the ion concentration (potential difference of 1 mV means a 4 % change in ion activity for a monovalent ion), the accuracy and precision of the measuring device (0.1 mV resolution, + /À 0.2 mV accuracy), the well-controlled measuring parameters such as cell temperature, electrode selectivity, and the presence of a graphene nanocomposite layer which significantly minimized the potential drift and enhanced the repeatability of the SC-ISE (minimum drift and complete reversibility without any memory) are important factors in achieving accurate and precise results of ISE.…”

Section: Potentiometric Sensors Characterizationsupporting

confidence: 82%

Graphene‐based Disposable Electrochemical Sensor for Chlorhexidine Determination

et al. 2022

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In this work, simple, reliable, eco‐friendly, and quantitative electrochemical sensors were developed to detect chlorhexidine Digluconate (CHX) in a variety of dosage forms, including mouthwashes and intimate douches, as well as chlorhexidine in spiked human saliva. Without any sample pre‐treatment or extraction processes, CHX was measured in colored aqueous formulations. Based on carbon screen‐printed electrodes, two potentiometric sensors (sensors I and II), utilizing graphene nanocomposites (Gr‐NC), were designed (SPEs). An ionophore, 2‐hydroxypropyl‐β‐cyclodextrin, was doped into the Poly Vinyl Chloride (PVC) polymeric membrane to improve sensor selectivity.

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Section: Potentiometric Sensors Characterizationsupporting

confidence: 82%

Graphene‐based Disposable Electrochemical Sensor for Chlorhexidine Determination

et al. 2022

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“…Under optimal settings, the relation was linear, as presented in Figure 6 : Ip(µA) = 0.20 C + 3.19, R 2 = 0.98 and Ip(µA) = 0.099 C + 10.52, R 2 = 0.99. The limits of detection (LOD = 3 σ/S) and quantification (LOQ = 10 σ/S) were 0.09 µM and 0.30 µM, correspondingly, where S is the calibration curve slope (0.099) and σ is the standard deviation of the blank (2.97 × 10 −3 ) [ 25 , 33 , 34 ]. The repeatability of the anticipated electrode was inspected by reiterating the tests five times under the matching circumstances, generating a relative standard deviation (RSD) of 1.31%.…”

Section: Resultsmentioning

confidence: 99%

An Innovative Simple Electrochemical Levofloxacin Sensor Assembled from Carbon Paste Enhanced with Nano-Sized Fumed Silica

Fekry

2022

Biosensors

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A new electrochemical sensor for the detection of levofloxacin (LV) was efficiently realized. The aim was to develop a new, cheap, and simple sensor for the detection of LV, which is used in various infections due to its pharmacological importance. It consists of carbon paste (CP) enhanced with nano-sized fumed silica (NFS). NFS has a very low bulk density and a large surface area. The carbon paste-enhanced NFS electrode (NFS/CPE) showed great electrocatalytic activity in the oxidation of 1.0 mM LV in Britton–Robinson buffer (BR) at pH values ranging from 3.0 to 8.0. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used; the peak current value (Ip) of the NFS/CPE sensor was 2.7 times that of the bare electrode, ensuring its high electrocatalytic activity. Electrochemical impedance spectroscopy (EIS) was performed at a peak potential (Ep) of +1066 mV, yielding a resistance of 10 kΩ for the designed NFS/CPE sensor compared to 2461 kΩ for the bare electrode, indicating the high conductivity of the modified sensor and verifying the data observed using the CV technique. Surface descriptions were determined by scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). The variation in the concentration of LV (2.0 to 1000 µM) was considered in BR buffer (pH = 5.0) at a scan rate (SR) of 10 mV/s by the NFS/CPE. The detection and quantification limits were 0.09 µM and 0.30 µM, respectively. To evaluate the application of LV in real samples, this procedure was established on Quinostarmax 500 mg tablets and human plasma samples. Reasonable results were obtained for the detection of LV.

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“…The synergic effects of l -lysine- o -PDA were the key to the excellent performance of the MIP as a sensitive element. El Azab et al 23 synthesized a poly(levodopa) and poly( o -phenylenediamine) composite onto gold nanoparticles. The composite was utilized as a sensor in the detection of levofloxacin.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensor Based on a Composite of Reduced Graphene Oxide and Molecularly Imprinted Copolymer of Polyaniline–Poly(o-phenylenediamine) for Ciprofloxacin Determination: Fabrication, Characterization, and Performance Evaluation

et al. 2023

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Contamination of antibiotics in water is a major cause of antibiotic resistance (ABR) in pathogens that endangers human health and food security worldwide. Ciprofloxacin (CIP) is a synthetic fluoroquinolone (FQ) antibiotic and is reportedly present in surface water at a concentration exceeding the ecotoxicological predicted no-effect concentration in some areas. This study fabricated a CIP sensor using an electropolymerized molecularly imprinted polymer (MIP) of polyaniline (PANI) and poly(o-phenylenediamine) (o-PDA) with CIP recognition sites. The MIP was coated on a reduced graphene oxide (rGO)modified glassy carbon electrode (rGO/GCE) and operated under a differential pulse voltammetry (DPV) mode for CIP detection. The sensor exhibited an excellent response from 1.0 × 10 −9 to 5.0 × 10 −7 mol L −1 CIP, showing a sensor detection limit and sensitivity of 5.28 × 10 −11 mol L −1 and 5.78 μA mol −1 L, respectively. The sensor's sensitivity for CIP was 1.5 times higher than that of the other tested antibiotics, including enrofloxacin (ENR), ofloxacin (OFX), sulfamethoxazole (SMZ), and piperacillin sodium salt (PIP). The reproducibility and reusability of the sensor devices were also studied.

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Point-of-care diagnostics for therapeutic monitoring of levofloxacin in human plasma utilizing electrochemical sensor mussel-inspired molecularly imprinted copolymer

Cited by 32 publications

References 71 publications

Graphene‐based Disposable Electrochemical Sensor for Chlorhexidine Determination

Graphene‐based Disposable Electrochemical Sensor for Chlorhexidine Determination

An Innovative Simple Electrochemical Levofloxacin Sensor Assembled from Carbon Paste Enhanced with Nano-Sized Fumed Silica

Electrochemical Sensor Based on a Composite of Reduced Graphene Oxide and Molecularly Imprinted Copolymer of Polyaniline–Poly(o-phenylenediamine) for Ciprofloxacin Determination: Fabrication, Characterization, and Performance Evaluation

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