Point-of-care genetic analysis for multiplex pathogenic bacteria on a fully integrated centrifugal microdevice with a large-volume sample

An Integrated Smartphone-Based Genetic Analyzer for Qualitative and Quantitative Pathogen Detection

“…Recent advancement of the genetic amplification technology as well as the miniaturized hardware and devices moves toward point-of-care (POC) testing for early diagnostics of pathogens. 3 In terms of the amplification technology, a variety of isothermal amplification methods have been developed including loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), nucleic acid sequence-based amplification (NASBA), and rolling circle amplification. These techniques require only one constant temperature for gene amplification, making the whole system simple for the POC DNA testing.…”

A review of smartphone point‐of‐care adapter design

“…It is evident that smartphones will dominate the POC field, enabling an easy‐to‐use mobile application that can perform complicated laboratory tests without the need for very deep knowledge about the instrumentation and the complicated test procedures used by specialists in a large laboratory environment . Recent innovations verified that smartphone‐based applications are very promising demonstrating precise measurements, a low limit of detection (LOD), a wide dynamic range, low power consumption, easy‐to‐use smart applications, a low coefficient of variation (CV%), a high regression coefficient (R 2 ), high specificity, reliable sensitivity, rapid testing, cost‐effective adapter designs, and high selectivity . These innovations include the major parts of POC applications, such as surgical treatment application (Section 3.2), surgical diagnosis (Section 3.1), ophthalmic applications (Section 3.3), biochemical applications (Section 3.4), environmental monitoring applications, biomedical applications, electrochemical applications (Section 3.5), colorimetric applications, imaging applications, and spectrometry applications .…”

“…13 Recent innovations verified that smartphone-based applications are very promising demonstrating precise measurements, [14][15][16][17][18] a low limit of detection (LOD), [19][20][21][22][23] a wide dynamic range, [24][25][26][27] low power consumption, easy-to-use smart applications, a low coefficient of variation (CV%), a high regression coefficient (R 2 ), high specificity, reliable sensitivity, rapid testing, cost-effective adapter designs, and high selectivity. [28][29][30][31][32] These innovations include the major parts of POC applications, such as surgical treatment application (Section 3.2), surgical diagnosis (Section 3.1), ophthalmic applications (Section 3.3), biochemical applications (Section 3.4), [33][34][35] environmental monitoring applications, [36][37][38][39][40][41][42] biomedical applications, [43][44][45] electrochemical applications (Section 3.5), colorimetric applications, [46][47][48][49] imaging applications, [50][51][52][53][54] and spectrometry applications. …”

A Fully Integrated<em> in vitro</em> Diagnostic Microsystem for Pathogen Detection Developed Using a &ldquo;3D Extensible&rdquo; Microfluidic Design Paradigm

“…As a result, a microfluidic device needs to handle at least 1 mL of sample, which is usually thousands of times higher than the volume of a typical microreactor (less than 1 μL) [22,23]. One solution is to attach enlarged reservoirs or tubes on microdevices to accommodate large-volume solutions [24,25]. However, these structures were only used as storage compartments and more complicated manipulations of large-volume reagents, such as mixing, have not been realized.…”