Point-of-care nucleic acid tests: assays and devices

Abstract

The COVID-19 pandemic (caused by the SARS_CoV_2 virus) has emphasized the need for quick, easy-to-operate, reliable, and affordable diagnostic tests and devices at the Point-of-Care (POC) for homes/fields/clinics. Such tests...

Quantitative and Multiplexed Chopper-Based Time-Gated Imaging for Bioanalysis on a Smartphone

“…Point-of-care diagnostics (POCDs) are widely recognized as essential for the future of health care. − Such technology has the potential to address diagnostic testing needs in communities that lack access to laboratory facilities and to support personalized medicine . Smartphones have emerged as an attractive platform for POCDs because of their global penetration, utility for quantitative measurements instead of qualitative assessments, and alignment with the “REASSURED” criteria (e.g., real-time connectivity, user-friendliness, and battery-powered operation).…”

A paper-embedded thermoplastic microdevice integrating additive-enhanced allele-specific amplification and silver nanoparticle-based colorimetric detection for point-of-care testing

“…However, laboratory-based diagnostic methods such as polymerase chain reaction (PCR) are unavailable in limited medical infrastructures such as in low-and middle-income countries and resource-limited environments where sophisticated equipment and trained operators are lacking. 1 In contrast, point-of-care testing (POCT) enables rapid on-site detection in these locations, moving the diagnostic tests outside of the traditional laboratory. 2,3 Among the various POCT approaches, such as protein, antigen, antibody detection, and nucleic acid tests (NATs), NATs exhibit the highest accuracy and specificity for numerous applications such as clinical diagnostics, food analysis, and environmental monitoring.…”

A Multichannel Fluorescence Isothermal Amplification Device with Integrated Internet of Medical Things for Rapid Sensing of Pathogens through Deep Learning

“…Isothermal nucleic acid amplification methodologies, such as loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), and helicase-dependent amplification (HAD), have gained prominence in molecular biology due to their unique advantages over conventional PCR techniques. − Benefiting from its rapid processing times, great simplicity, enhanced sensitivity, and increased portability, isothermal amplification has been demonstrated as an ideal tool for field-based applications, particularly in remote or resource-limited environments. − Therefore, the integration of isothermal amplification-based POCT into clinical settings is imperative for the enhancement of global health and the containment of future pandemics in diverse applications, including molecular diagnostics, environmental monitoring, and biodefense. − For instance, isothermal-amplification-based POCT technologies have been extensively used to rapidly detect various pathogens such as bacteria and viruses. − …”