Point-of-care (POC) diagnosis of bacterial vaginosis (BV) using VGTest™ ion mobility spectrometry (IMS) in a routine ambulatory care gynecology clinic

Blankenstein, Thomas; Lytton, Simon D.; Leidl, B.; Atweh, Elias; Friese, Klaus; Mylonas, Ioannis

doi:10.1007/s00404-014-3613-x

Cited by 20 publications

(12 citation statements)

References 28 publications

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“…Several POC diagnostic assays exist to diagnose BV, such as saline microscopy, wet mount microscopy, chromogenic tests such as the OSOM® BVBlue® and the VGTest™ ion motility spectrometry (IMS). The VGTest™ IMS determines the levels of the malodorous biogenic amines associated with BV, whereas the OSOM® BVBlue® test detects elevated levels of the sialidase enzyme to diagnose BV (Madhivanan et al, 2014 ; Blankenstein et al, 2015 ). Rapid assays detecting the presence of proline amino peptidase in BV have also demonstrated high levels of specificity and sensitivity (Madhivanan et al, 2014 ).…”

Section: Current Diagnostic Avenuesmentioning

confidence: 99%

Bacterial Vaginosis: Current Diagnostic Avenues and Future Opportunities

Redelinghuys

Geldenhuys

Jung

et al. 2020

Front. Cell. Infect. Microbiol.

117

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A healthy female genital tract harbors a microbiome dominated by lactic acid and hydrogen peroxide producing bacteria, which provide protection against infections by maintaining a low pH. Changes in the bacterial compositions of the vaginal microbiome can lead to bacterial vaginosis (BV), which is often associated with vaginal inflammation. Bacterial vaginosis increases the risk of acquiring sexually transmitted infections (STIs) like human immunodeficiency virus (HIV) and affects women's reproductive health negatively. In pregnant women, BV can lead to chorioamnionitis and adverse pregnancy outcomes, including preterm premature rupture of the membranes and preterm birth. In order to manage BV effectively, good diagnostic procedures are required. Traditionally clinical and microscopic methods have been used to diagnose BV; however, these methods require skilled staff and time and suffer from reduced sensitivity and specificity. New diagnostics, including highly sensitive and specific point-of-care (POC) tests, treatment modalities and vaccines can be developed based on the identification of biomarkers from the growing pool of vaginal microbiome and vaginal metabolome data. In this review the current and future diagnostic avenues will be discussed.

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Section: Current Diagnostic Avenuesmentioning

confidence: 99%

Bacterial Vaginosis: Current Diagnostic Avenues and Future Opportunities

Redelinghuys

Geldenhuys

Jung

et al. 2020

Front. Cell. Infect. Microbiol.

117

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“…One clinical feature of BV, malodor, has been linked to increases in vaginal biogenic amines (BAs), including the polyamines putrescine, cadaverine, and trimethylamine (Yeoman et al, 2013 ). BAs are organic compounds with one or more amine (NH 2 ) group(s), and may represent useful biomarkers of BV (Blankenstein et al, 2015 ). Other common BAs include tyramine, agmatine, spermine, and spermidine, the latter having also been observed at low relative concentrations in the vaginal metabolome (Yeoman et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Vaginal biogenic amines: biomarkers of bacterial vaginosis or precursors to vaginal dysbiosis?

et al. 2015

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Bacterial vaginosis (BV) is the most common vaginal disorder among reproductive age women. One clinical indicator of BV is a “fishy” odor. This odor has been associated with increases in several biogenic amines (BAs) that may serve as important biomarkers. Within the vagina, BA production has been linked to various vaginal taxa, yet their genetic capability to synthesize BAs is unknown. Using a bioinformatics approach, we show that relatively few vaginal taxa are predicted to be capable of producing BAs. Many of these taxa (Dialister, Prevotella, Parvimonas, Megasphaera, Peptostreptococcus, and Veillonella spp.) are more abundant in the vaginal microbial community state type (CST) IV, which is depleted in lactobacilli. Several of the major Lactobacillus species (L. crispatus, L. jensenii, and L. gasseri) were identified as possessing gene sequences for proteins predicted to be capable of putrescine production. Finally, we show in a small cross sectional study of 37 women that the BAs putrescine, cadaverine and tyramine are significantly higher in CST IV over CSTs I and III. These data support the hypothesis that BA production is conducted by few vaginal taxa and may be important to the outgrowth of BV-associated (vaginal dysbiosis) vaginal bacteria.

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“…As expected, VOC analysis did detect BV as this condition is known to be associated with specific odour. This finding could have some clinical utility in a rapid bedside diagnostic test for BV and this concept is currently being developed by others 38 .…”

Section: Discussionmentioning

confidence: 92%

Volatile organic compound analysis, a new tool in the quest for preterm birth prediction—an observational cohort study

Lacey

Daulton

Wicaksono

et al. 2020

Sci Rep

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Preterm birth is the leading cause of death worldwide in children under five years. Due to its complex multifactorial nature, prediction is a challenge. current research is aiming to develop accurate predictive models using patient history, ultrasound and biochemical markers. Volatile organic compound (Voc) analysis is an approach, which has good diagnostic potential to predict many disease states. Analysis of VOCs can reflect both the microbiome and host response to a condition. We aimed to ascertain if VOC analysis of vaginal swabs, taken throughout pregnancy, could predict which women go on to deliver preterm. our prospective observational cohort study demonstrates that VOC analysis of vaginal swabs, taken in the midtrimester, is a fair test (AUC 0.79) for preterm prediction, with a sensitivity of 0.66 (95%CI 0.56-0.75) and specificity 0.89 (95%CI 0.82-0.94). Using vaginal swabs taken closest to delivery, VOC analysis is a good test (AUC 0.84) for the prediction of preterm birth with a sensitivity of 0.73 (95%CI 0.64-0.81) and specificity of 0.90 (95%CI 0.82-0.95). Consequently, VOC analysis of vaginal swabs has potential to be used as a predictive tool. With further work it could be considered as an additional component in models for predicting preterm birth.

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Point-of-care (POC) diagnosis of bacterial vaginosis (BV) using VGTest™ ion mobility spectrometry (IMS) in a routine ambulatory care gynecology clinic

Abstract: VGTest-IMS is accurate and feasible for point-of-care testing of BV in the ambulatory care setting. Further evaluations are in progress to assess the utility of VGTest-IMS for differential diagnosis of candidosis, non-BV infection and common inflammatory conditions.

Cited by 20 publications

References 28 publications

Bacterial Vaginosis: Current Diagnostic Avenues and Future Opportunities

Bacterial Vaginosis: Current Diagnostic Avenues and Future Opportunities

Vaginal biogenic amines: biomarkers of bacterial vaginosis or precursors to vaginal dysbiosis?

Volatile organic compound analysis, a new tool in the quest for preterm birth prediction—an observational cohort study

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