2022
DOI: 10.3389/fvets.2022.1056440
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Point-of-care testing in companion and food animal disease diagnostics

Abstract: Laboratory diagnoses of animal diseases has advanced tremendously in recent decades with the advent of cutting-edge technologies such as real-time polymerase chain reaction, next generation sequencing (NGS), matrix-assisted laser desorption/ionization time—of—flight mass spectrometry (MALDI-TOF MS) and others However, most of these technologies need sophisticated equipment, laboratory space and highly skilled workforce. Therefore, there is an increasing market demand for point-of-care testing (POCT) in animal … Show more

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Cited by 15 publications
(12 citation statements)
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“…This variation underscores the need for tailored approaches in disease management. While most POCTs for food animal diseases provide simple dichotomous results, there are also tests that offer more detailed quantitative or semi-quantitative assessments, providing a deeper understanding of disease status ( 16 , 34 , 35 ). The availability of continuous results presents a valuable opportunity for veterinary services to validate different cut-off values based on animal species and sample type, thereby improving specificity and accuracy in disease diagnosis and management.…”
Section: Discussionmentioning
confidence: 99%
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“…This variation underscores the need for tailored approaches in disease management. While most POCTs for food animal diseases provide simple dichotomous results, there are also tests that offer more detailed quantitative or semi-quantitative assessments, providing a deeper understanding of disease status ( 16 , 34 , 35 ). The availability of continuous results presents a valuable opportunity for veterinary services to validate different cut-off values based on animal species and sample type, thereby improving specificity and accuracy in disease diagnosis and management.…”
Section: Discussionmentioning
confidence: 99%
“…The operational performance of a diagnostic test encompasses factors beyond accuracy alone, including precision, speed, ease of use, cost-effectiveness, robustness, and suitability for the intended purpose or specific context/population. Studies by Hobbs et al ( 5 ) and Velayudhan and Naikare ( 16 ) have discussed these challenges extensively and highlighted issues such as inconsistent and unclear validation data from test manufacturers, limitations in field validation and evaluation studies, difficulties in validating tests for specific pathogens, and the lack of guidelines for ensuring quality control in veterinary POCTs.…”
Section: Why Is It Relevant To Standardize and Harmonize Criteria For...mentioning
confidence: 99%
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“…Alternatively, point-of-care diagnostic techniques have been proposed as an option to overcome these drawbacks as they can be easily implemented in small and medium-sized livestock centers. In this sense, LAMP has been suggested as a rapid and low-cost method for the detection and surveillance of M. bovis in livestock and humans in resource-limited endemic areas where only basic laboratories are available [ 17 , 30 ]. Previously, Kapalamula et al.…”
Section: Methodsmentioning
confidence: 99%
“…The diagnostic/prognostic tests performed out of the laboratory and near/at the site of patient are termed as point-of-care-tests (POCTs) as per the definition provided by the International Standard ISO 22870 [ 1 ]. These tests have a rapid turnaround time, and assist in rapid decision-making and faster care of the patient.…”
Section: Introductionmentioning
confidence: 99%