Point-of-care testing of infectious diseases: recent advances

Abstract

Infectious diseases have seriously threatened human health and caused enormous losses to the global economy. Rapid and accurate diagnosis of pathogens is crucial to the timely treatment of patients, improvement...

A FokI-driven signal amplification platform for the simultaneous detection of multiple viral RNA pathogens

“…33,34 In this regard, the development of new diagnostic methodologies as alternatives to the PCR technique has led to the implementation of diagnostic tests centred on the use of new tools based on LAMP, RPA, or CRISPR/Cas9, among others. [35][36][37] Some of these methodologies are also highly portable to point-of-care (POC) systems, 38,39 which could prove effective in responding to critical situations, whether in remote locations or with limited equipment, or when facing reagent shortages associated with the PCR technique. 40 To address these limitations and expand the catalogue of available diagnostic methodologies, this work focused on implementing recent advances observed in the context of signal amplification strategies 24 with classical nucleic acid amplification technologies such as the RCA technique.…”

Advances in electrochemical sensors for real-time glucose monitoring

“…3 Wearable biosensors, with a focus on sweat and interstitial fluid (ISF) as abundant sources of biochemical markers, 4,5 offer pioneering avenues for management as well as early disease detection and personalized point-of-care testing (POCT). [6][7][8][9][10] These sensors afford real-time physiological data acquisition through noninvasive means, enabling continuous monitoring round the clock. 11 For instance, the accurate surveillance of hypoglycemia or hyperglycemia via sweat is essential for diabetic patients, as fluctuations in blood glucose levels pose significant health risks.…”

Extending the Shelf-Life of Immunoassay-Based Microfluidic Chips through Freeze-Drying Sublimation Techniques

“…Protein conformations and antibody functionalities are highly sensitive to their surrounding environments [13,33]. As a result, the surface chemistry in the channels of these devices tends to degrade over time, particularly when exposed to harsh conditions like high temperatures and high humidity, common in tropical regions where they are frequently utilized for POCT [34].…”