Point-of-care treatment of geometrically complex midfacial critical-sized bone defects with 3D-Printed scaffolds and autologous stromal vascular fraction

Singh, Srujan; Nyberg, Ethan L.; O'Sullivan, Aine N.; Farris, Ashley L.; Rindone, Alexandra N.; Zhang, Nicholas; Whitehead, Emma C.; Zhou, Yuxiao; Mihaly, Eszter; Achebe, Chukwuebuka C.; Zbijewski, Wojciech; Grundy, W. M.; Garlick, David S.; Jackson, Nicolette; Taguchi, Takashi; Takawira, Catherine; Lopez, Joseph; Lopez, Mandi J.; Grant, Michael P.; Grayson, Warren L.

doi:10.1016/j.biomaterials.2022.121392

Cited by 13 publications

(15 citation statements)

References 33 publications

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“…Isolation of SVF from Subcutaneous Porcine Fat: As previously described [20] under anesthesia, subcutaneous adipose tissue was excised from the lumbar paravertebral area. Briefly, following aseptic preparation a skin incision was made over the area superficial to L3-L6 using a #10 blade and subcutaneous adipose tissue was sharply excised.…”

Section: Methodsmentioning

confidence: 99%

“…Specifically, the majority of newly deposited mineral appeared to originate from the adjacent native bone and grew around the scaffolds (osteoconductive bone) while smaller nodules accumulated by nucleating around the struts in the scaffold interior (osteoinductive bone) (Figure S1, Supporting Information). [20] Understanding the spatial patterns of scaffold-mediated bone growth has significant implications for adequately and effectively treating midfacial bone injuries where the anatomic geometry is critical to function and aesthetics. Orthotopic bone regeneration is necessary for anatomically correct reconstruction as any major ectopic bony formation in the periorbital region may negatively affect vision.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Geometric Mismatch Promotes Anatomic Repair in Periorbital Bony Defects in Skeletally Mature Yucatan Minipigs

Singh,

Zhou,

Farris

et al. 2023

Adv Healthcare Materials

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Porous tissue‐engineered 3D‐printed scaffolds are a compelling alternative to autografts for the treatment of large periorbital bone defects. Matching the defect‐specific geometry has long been considered an optimal strategy to restore pre‐injury anatomy. However, studies in large animal models have revealed that biomaterial‐induced bone formation largely occurs around the scaffold periphery. Such ectopic bone formation in the periorbital region can affect vision and cause disfigurement. To enhance anatomic reconstruction, we introduced geometric mismatches in the scaffolds used to treat full thickness zygomatic defects created bilaterally in adult Yucatan minipigs. We used 3D‐printed, anatomically‐mirrored scaffolds in combination with autologous stromal vascular fraction of cells (SVF) for treatment. We developed an advanced image‐registration workflow to quantify the post‐surgical geometric mismatch and correlate it with the spatial pattern of the regenerating bone. Osteoconductive bone growth on the dorsal and ventral aspect of the defect enhanced scaffold integration with the native bone while medio‐lateral bone growth led to failure of the scaffolds to integrate. We found a strong positive correlation between geometric mismatch and orthotopic bone deposition at the defect site. The data suggested that strategic mismatch >20% could improve bone scaffold design to promote enhanced regeneration, osseointegration and long‐term scaffold survivability.This article is protected by copyright. All rights reserved

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Geometric Mismatch Promotes Anatomic Repair in Periorbital Bony Defects in Skeletally Mature Yucatan Minipigs

Singh,

Zhou,

Farris

et al. 2023

Adv Healthcare Materials

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“…SVF is an easily accessible and readily available source of mesenchymal stem cells that can be extracted from autologous lipoaspirate tissue. Compared to acellular PCL-DCB scaffolds, the SVF group demonstrated superior osteointegration after 1-year in vivo , as deduced by requiring a significantly higher torque to fracture the bone-scaffold interface . This approach to bone regeneration is particularly appealing because, if clinically translated, the SVF could be harvested and extracted intraoperatively from patient’s adipose tissue.…”

Section: Biomaterials For 3d Printed Bony Defect Repair Of the Cmf Sk...mentioning

confidence: 99%

“…75 PCL has also been evaluated as a candidate for craniofacial defect repair in several in vivo studies. 86,91 Recently, the effect of oxygen loading on vascularization and bone regeneration was evaluated in critical-sized calvarial defects of a murine model. 86 Biodegradable synthetic microtanks housed within a porous ASC-seeded PCL-scaffold were hyperbarically loaded with pure oxygen.…”

Section: Biomaterials For 3d Printed Bony Defectmentioning

confidence: 99%

“…Compared to acellular PCL-DCB scaffolds, the SVF group demonstrated superior osteointegration after 1-year in vivo, as deduced by requiring a significantly higher torque to fracture the bone-scaffold interface. 91 This approach to bone regeneration is particularly appealing because, if clinically translated, the SVF could be harvested and extracted intraoperatively from patient's adipose tissue. Moreover, as an autologous cell source with osteogenic potential, SVF-infused scaffolds may supply the benefits associated with an autograft without the comorbidities.…”

Section: Biomaterials For 3d Printed Bony Defectmentioning

confidence: 99%

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3D Printing Applications for Craniomaxillofacial Reconstruction: A Sweeping Review

Slavin,

Ehlen,

Costello

et al. 2023

ACS Biomater. Sci. Eng.

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Bifunctional Piezo‐Enhanced PLLA/ZA Coating Prevents Aseptic Loosening of Bone Implants

Cui,

Shan,

et al. 2024

Adv Funct Materials

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Prevention of aseptic loosening, the main reason for secondary surgery of bone implants, is of great crucial clinical importance. As a promising bioactive material to promote bone regeneration, the piezoelectric materials for preventing aseptic loosening has not been reported. Here, a surface coating is presented for implants based on piezoelectric poly(L‐lactic acid) (PLLA). The coating ingeniously utilizes zoledronic acid (ZA), a drug used to treat osteoporosis, as a nucleating agent to enhance the piezoelectricity of PLLA. The coating can not only provide a continuous porous piezoelectric environment to stimulate osteogenic differentiation without intervention, but also effectively inhibit the osteolytic activity of osteoclasts through the slow release of ZA, achieving long‐term effectiveness with a single implantation. The rat femur cavity implantation experiment suggests that, the PLLA/ZA coating can rapidly initiate osseointegration of the implant and effectively mitigate the risk of aseptic loosening, even in the presence of metal submicroscopic particles that induce aseptic loosening. This strategy of piezo‐enhanced PLLA drug‐loaded coating may provide a new idea for the surface modification of bone implants.

show abstract

Point-of-care treatment of geometrically complex midfacial critical-sized bone defects with 3D-Printed scaffolds and autologous stromal vascular fraction

Cited by 13 publications

References 33 publications

Geometric Mismatch Promotes Anatomic Repair in Periorbital Bony Defects in Skeletally Mature Yucatan Minipigs

Geometric Mismatch Promotes Anatomic Repair in Periorbital Bony Defects in Skeletally Mature Yucatan Minipigs

3D Printing Applications for Craniomaxillofacial Reconstruction: A Sweeping Review

Bifunctional Piezo‐Enhanced PLLA/ZA Coating Prevents Aseptic Loosening of Bone Implants

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