Polar contributions of the stationary phase to the reversed-phase ion-pair high-performance liquid chromatographic separation of quaternary ammonium drugs

Schutter, J.A. De; Moerloose, P. De

doi:10.1016/s0021-9673(00)90372-8

Cited by 22 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is reported that dioscin induces OS cell apoptosis by upregulating ROS-mediated P38 MAPK signaling [ 30 ], and Fan’s research also showed that siglec-15 promotes tumor progression in OS via DUSP1/MAPK pathway [ 31 ]. Similarly, the role of PI3K/AKT pathway in reversing drug resistance in OS is confirmed by the examination of some reports [ 32 , 33 ]. However, how GPR65 functions through MAPK and PI3K/AKT pathways in OS not yet clear, and we will address this issue in future studies.…”

Section: Discussionmentioning

confidence: 60%

What role does GPR65 play in the progression of osteosarcoma? Its mechanism and clinical significance

Qi,

Liu,

Zhang

2024

Cancer Cell Int

View full text Add to dashboard Cite

Background GPR65 is a pH-sensing G-protein-coupled receptor that acts as a key innate immune checkpoint in the human tumor microenvironment, inhibiting the release of inflammatory factors and inducing significant upregulation of tissue repair genes. However, the expression pattern and function of GPR65 in osteosarcoma (OS) remain unclear. The purpose of this study was to investigate and elucidate the role of GPR65 in the microenvironment, proliferation and migration of OS. Methods Retrospective RNA-seq data analysis was conducted in a cohort of 97 patients with OS data in the TAEGET database. In addition, single-cell sequencing data from six surgical specimens of human OS patients was used to analyze the molecular evolution process during OS genesis. Tissues chips and bioinformatics results were used to verify GPR65 expression level in OS. MTT, colony formation, EdU assay, wound healing, transwell assay and F-actin assay were utilized to analyze cell proliferation and invasion of OS cancer cells. RNA-seq was used to explore the potential mechanism of GPR65’s role in OS. Results GPR65 expression was significantly low in OS, and subgroup analysis found that younger OS patients, OS patients in metastatic status, and overall survival and progression free survival OS patients had lower GPR65 expression. From ScRNA-seq data of GSE162454, we found the expression of GPR65 is significantly positively correlated with CD4 + T cells CD8 + T cells and OS related macrophage infiltration. Verification experiment found that silencing the expression of GPR65 in osteosarcoma cells U2OS and HOS could promote the proliferation and invasion process, RNA-seq results showed that the role of GPR65 in OS cells was related to immune system, metabolism and signal transduction. Conclusion The low expression of GPR65 in OS leads to high metastasis rate and poor prognosis in OS patients. The suppression of immune escape and inhibition of proliferation may be a key pathway for GPR65 to participate in the progression of OS. The current study strengthens the role of GPR65 in OS development and provides a potential biomarker for the prognosis of OS patients.

show abstract

Section: Discussionmentioning

confidence: 60%

What role does GPR65 play in the progression of osteosarcoma? Its mechanism and clinical significance

Qi,

Liu,

Zhang

2024

Cancer Cell Int

View full text Add to dashboard Cite

show abstract

“…Reproducible results with high resolving power were reported by simultaneous usage of them in the study of quaternary ammonium drugs [16] and some basic drugs [17][18][19].…”

Section: Introductionmentioning

confidence: 78%

An efficient separation and method development for the quantifying of two basic impurities of Nicergoline by reversed-phase high performance liquid chromatography using ion-pairing counter ions

Yalçın

Yüktaş

2006

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

“…The adopted solutions in the literature are focused on the improvement of the stationary phase (encapping, base-deactivated column, etc.) and modification of the mobile phase using buffer, amine modifiers, ion-pair reagent (20)(21)(22)(23)(24)(25)(26)(27)(28)(29), and divalent metals (30). These modifications block stationary phase silanol groups from silanophilic contribution, reducing or eliminating this undesirable effect.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Residual Products in Triethylbenzylammonium Chloride by HPLC. Study of the Retention Mechanism

Prieto-Blanco

López-Mahı́a

Prada‐Rodríguez

2006

Journal of Chromatographic Science

View full text Add to dashboard Cite

The control of industrial products for minimization of their impact on the environment and human health requires the development of specific analysis methods. Information provided by these methods about toxic components, by-products, and other derivatives may also be useful to reduce the possible impact of industrial products. The studied compound in this paper, triethylbenzylammonium chloride (TEBA), is mainly used in industrial synthesis. This quaternary compound and its residual products coming from quaternization reaction (benzyl chloride, benzaldehyde, and benzyl alcohol) are analyzed by HPLC. The separation is based on control of the silanophilic contribution to TEBA retention because of the quaternary nature of this compound. The effect of the three buffers (sodium acetate, ammonium acetate, and sodium formate) and their concentrations in the chromatographic behavior of the quaternary compound is examined. The buffer cation and anion regulate TEBA retention. Also, the concentration of the quaternary compound is another parameter that had influence in some aspects of its chromatographic behavior (e.g., retention and symmetry). The proposed method is applied to TEBA synthesis along, with the formation and removal of impurities with the results compared with those obtained for the quaternary compound benzalkonium chloride.

show abstract

Polar contributions of the stationary phase to the reversed-phase ion-pair high-performance liquid chromatographic separation of quaternary ammonium drugs

Cited by 22 publications

References 25 publications

What role does GPR65 play in the progression of osteosarcoma? Its mechanism and clinical significance

What role does GPR65 play in the progression of osteosarcoma? Its mechanism and clinical significance

An efficient separation and method development for the quantifying of two basic impurities of Nicergoline by reversed-phase high performance liquid chromatography using ion-pairing counter ions

Analysis of Residual Products in Triethylbenzylammonium Chloride by HPLC. Study of the Retention Mechanism

Contact Info

Product

Resources

About