Polar Localization of a Soluble Methyl-Accepting Protein of<i>Pseudomonas aeruginosa</i>

Bardy, Sonia L.; Maddock, Janine R.

doi:10.1128/jb.187.22.7840-7844.2005

Cited by 37 publications

(29 citation statements)

References 31 publications

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“…Phosphorylation of CheB activates its esterase activity and leads to demethylation of chemoreceptors, diminishing their capacity to activate CheA (16,32,33). A similar system appears to operate in at least some cytoplasmic chemoreceptors, based on the observation that these cytoplasmic chemoreceptors possess a methylation consensus in the adaptation region (15,(34)(35)(36). In most cases, however, methylation has not been confirmed, nor is the specific role of methylation known.…”

Section: Adaptation In Chemoreceptorsmentioning

confidence: 99%

“…There have been no studies on the proteins required for McpS PA polar localization. Taken together, this cytoplasmic chemoreceptor appears to reside in a polar complex with transmembrane chemoreceptors, and this macromolecular structure is sensitive to the concentration of McpS PA , perhaps through displacing interactions between receptors or signaling proteins (34).…”

Section: Cytoplasmic Chemoreceptors With Nearly Exclusive Localizatiomentioning

confidence: 99%

“…McpS PA contains an N-terminal PAS domain and predicted methylation sites, but it is not yet known what it responds to or whether it is methylated (34). McpS PA localizes to the pole along with several other chemoreceptors, based on immunofluorescence (34). Increasing McpS PA levels disrupted the polar localization of transmembrane chemoreceptors, but McpS PA was still observed at the pole.…”

Section: Cytoplasmic Chemoreceptors With Nearly Exclusive Localizatiomentioning

confidence: 99%

“…The P. aeruginosa PAO1 genome encodes 4 chemotaxis pathways with 26 chemoreceptors, 3 of which lack transmembrane regions and are therefore predicted to be soluble (34). McpS PA contains an N-terminal PAS domain and predicted methylation sites, but it is not yet known what it responds to or whether it is methylated (34). McpS PA localizes to the pole along with several other chemoreceptors, based on immunofluorescence (34).…”

Section: Cytoplasmic Chemoreceptors With Nearly Exclusive Localizatiomentioning

confidence: 99%

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Internal Sense of Direction: Sensing and Signaling from Cytoplasmic Chemoreceptors

Collins

Lacal

Ottemann

2014

Microbiol Mol Biol Rev

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SUMMARY Chemoreceptors sense environmental signals and drive chemotactic responses in Bacteria and Archaea . There are two main classes of chemoreceptors: integral inner membrane and soluble cytoplasmic proteins. The latter were identified more recently than integral membrane chemoreceptors and have been studied much less thoroughly. These cytoplasmic chemoreceptors are the subject of this review. Our analysis determined that 14% of bacterial and 43% of archaeal chemoreceptors are cytoplasmic, based on currently sequenced genomes. Cytoplasmic chemoreceptors appear to share the same key structural features as integral membrane chemoreceptors, including the formations of homodimers, trimers of dimers, and 12-nm hexagonal arrays within the cell. Cytoplasmic chemoreceptors exhibit varied subcellular locations, with some localizing to the poles and others appearing both cytoplasmic and polar. Some cytoplasmic chemoreceptors adopt more exotic locations, including the formations of exclusively internal clusters or moving dynamic clusters that coalesce at points of contact with other cells. Cytoplasmic chemoreceptors presumably sense signals within the cytoplasm and bear diverse signal input domains that are mostly N terminal to the domain that defines chemoreceptors, the so-called MA domain. Similar to the case for transmembrane receptors, our analysis suggests that the most common signal input domain is the PAS (Per-Arnt-Sim) domain, but a variety of other N-terminal domains exist. It is also common, however, for cytoplasmic chemoreceptors to have C-terminal domains that may function for signal input. The most common of these is the recently identified chemoreceptor zinc binding (CZB) domain, found in 8% of all cytoplasmic chemoreceptors. The widespread nature and diverse signal input domains suggest that these chemoreceptors can monitor a variety of cytoplasmically based signals, most of which remain to be determined.

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Section: Adaptation In Chemoreceptorsmentioning

confidence: 99%

Section: Cytoplasmic Chemoreceptors With Nearly Exclusive Localizatiomentioning

confidence: 99%

Section: Cytoplasmic Chemoreceptors With Nearly Exclusive Localizatiomentioning

confidence: 99%

Section: Cytoplasmic Chemoreceptors With Nearly Exclusive Localizatiomentioning

confidence: 99%

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Internal Sense of Direction: Sensing and Signaling from Cytoplasmic Chemoreceptors

Collins

Lacal

Ottemann

2014

Microbiol Mol Biol Rev

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“…This process results in a nonuniform distribution of signaling arrays at cell poles and randomly along the cell length (10). In other organisms chemosensory arrays are localized only to the cell poles (11)(12)(13)(14); however, the mechanisms behind polar localization are incompletely understood.…”

mentioning

confidence: 99%

ParP prevents dissociation of CheA from chemotactic signaling arrays and tethers them to a polar anchor

Ringgaard

Zepeda-Rivera

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Significance Targeting of cellular components to a particular site in a cell is often a highly regulated process, even in cells as small as bacteria. Robust chemotactic signaling, which is used by motile bacteria to survey their environments and navigate in response to them, requires appropriate cellular distribution of a large chemosensory apparatus. Here, we report how polarly flagellated vibrios ensure polar localization of their chemotactic machinery by capturing signaling proteins at the pole. Polar localization is mediated by a tripartite protein interaction network in which one protein prevents disassociation of a key signaling component from chemotactic complexes and tethers the complexes to a polar anchor. Polar tethering and localization are prerequisites for proper chemotaxis.

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PseudomonasMotility and Chemotaxis

Kato

2008

Pseudomonas

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Polar Localization of a Soluble Methyl-Accepting Protein ofPseudomonas aeruginosa

Cited by 37 publications

References 31 publications

Internal Sense of Direction: Sensing and Signaling from Cytoplasmic Chemoreceptors

Internal Sense of Direction: Sensing and Signaling from Cytoplasmic Chemoreceptors

ParP prevents dissociation of CheA from chemotactic signaling arrays and tethers them to a polar anchor

PseudomonasMotility and Chemotaxis

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