Polar Polymeric Stationary Phases for Normal-Phase HPLC Based on Monodisperse Macroporous Poly(2,3-dihydroxypropyl methacrylate-co-ethylene dimethacrylate) Beads

Abstract

The effect of variables such as shape template size, porogen composition and percentage, content of cross-linking monomer, and polymerization temperature on the properties of uniformly sized 3-microm porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads prepared by the staged templated suspension polymerization technique has been studied. The porous properties of the beads including surface morphology, pore size distribution, and specific surface area have been optimized to obtain highly efficient stationary phases for normal-phase HPLC. A column packed with diol stationary phase obtained by hydrolysis of poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads affords an efficiency of 67,000 plates/m for toluene using THF as the mobile phase. The retention properties and selectivity of the diol beads are easily modulated by changes in the composition of the mobile phase. The performance of these beads is demonstrated with the separations of a variety of polar compounds including positional isomers, aniline derivatives, and basic tricyclic antidepressant drugs.

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

“…Particles prepared for chromatography columns are packed into columns and back pressure is measured as an indication of mechanical strength for instance [300].…”

Preparation of normal‐phase HPLC stationary phase based on monodisperse hydrophilic polymeric beads and their application

“…This is much lower than the reported method. 11 This result demonstrates that the beads possess high permeability.…”

“…The testing of the organic polymer matrixes under normal-phase conditions is seldom reported. 8,9 Petro et al 10 and Xu et al 11 reported based on linear polystyrene beads (preparation by emulsification polymerization method) as seeds for preparation of macroporous poly(glycidymethacrylate-co-ethylenedimethacrylate) (P GMA/EDMA ) beads by using a two-step swelling and polymerization; then the hydrolysis of the beads were used as the NPLC stationary phase for the separation of a variety of polar compounds.…”

Synthesis of monodisperse poly(chloromethyl-styrene-co-divinylbenzene) beads and their application in separation of biopolymers

“…Therefore, we use mixtures of the two porogens to avoid formation of surface holes and simultaneously obtain beads with a smoother surface [20].…”