Polarization of PI3K Activity Initiated by Ooplasmic Segregation Guides Nuclear Migration in the Mesendoderm

Takatori, Naohito; Oonuma, Kouhei; Nishida, Hiroki; Saiga, Hidetoshi

doi:10.1016/j.devcel.2015.10.012

Cited by 11 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detection of nodal mRNA and the active form of Arp2/3 complex, as well as of bmp2/4 mRNA and phosphorylated Smad1 (pSmad1) was performed. Riboprobes were first detected by TSA Plus cyanine 3/fluorescein system (PerkinElmer, MA) as described previously [ 11 ]. After the completion of WISH, the specimens were subjected to immunostaining.…”

Section: Methodsmentioning

confidence: 99%

“…Ascidians also display ooplasmic dynamics more remarkably than anamniote vertebrates do, however they do not specify the dorsal fate but the posterior fate by this process [ 8 , 9 ]. Dorsal structures are induced by asymmetrical segregation of mRNAs during cleavage and Fgf signalling emanating from endodermal precursor cells [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Acquisition of the dorsal structures in chordate amphioxus

et al. 2016

View full text Add to dashboard Cite

Acquisition of dorsal structures, such as notochord and hollow nerve cord, is likely to have had a profound influence upon vertebrate evolution. Dorsal formation in chordate development thus has been intensively studied in vertebrates and ascidians. However, the present understanding does not explain how chordates acquired dorsal structures. Here we show that amphioxus retains a key clue to answer this question. In amphioxus embryos, maternal nodal mRNA distributes asymmetrically in accordance with the remodelling of the cortical cytoskeleton in the fertilized egg, and subsequently lefty is first expressed in a patch of blastomeres across the equator where wnt8 is expressed circularly and which will become the margin of the blastopore. The lefty domain co-expresses zygotic nodal by the initial gastrula stage on the one side of the blastopore margin and induces the expression of goosecoid, not-like, chordin and brachyury1 genes in this region, as in the oral ectoderm of sea urchin embryos, which provides a basis for the formation of the dorsal structures. The striking similarity in the gene regulations and their respective expression domains when comparing dorsal formation in amphioxus and the determination of the oral ectoderm in sea urchin embryos suggests that chordates derived from an ambulacrarian-type blastula with dorsoventral inversion.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Acquisition of the dorsal structures in chordate amphioxus

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Their many advantages as laboratory organisms have carried the tunicates through the centuries of experimental biology, and their status as the sister group to the vertebrates has helped secure a spot for them at the biomedical research table. Studies in tunicates have helped establish basic concepts in developmental biology such as invariant lineages and mosaic development [64, 66, 191, 352], and have shed light on transcriptional and cellular mechanisms of development [26, 59, 68, 69, 84, 101, 136, 167, 226, 234, 262, 265, 323]. Furthermore, comparative studies using tunicates have refined models of chordate and vertebrate evolution [1, 2, 9, 49, 92, 112, 156, 192, 205, 275, 311, 345].…”

Section: Introductionmentioning

confidence: 99%

Regulation and evolution of muscle development in tunicates

Razy-Krajka

Stolfi

2019

EvoDevo

View full text Add to dashboard Cite

For more than a century, studies on tunicate muscle formation have revealed many principles of cell fate specification, gene regulation, morphogenesis, and evolution. Here, we review the key studies that have probed the development of all the various muscle cell types in a wide variety of tunicate species. We seize this occasion to explore the implications and questions raised by these findings in the broader context of muscle evolution in chordates.

show abstract

“…In turn, tumor cell-produced ligands such as colony-stimulating factor 1 (CSF1) signal to CSF1R on TAMs to promote recruitment and M2-like polarization 41 , 43 . Downstream PI3K signaling is implicated in such signaling and MT dynamics 44 , 45 , and in TAMs, reports show PI3K inhibition (particularly of isoform p110γ) reprograms cells toward M1-like polarization 46 .…”

Section: Resultsmentioning

confidence: 99%

In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells

Luthria

Wang

et al. 2020

Nat Commun

View full text Add to dashboard Cite

Microtubules (MTs) mediate mitosis, directional signaling, and are therapeutic targets in cancer. Yet in vivo analysis of cancer cell MT behavior within the tumor microenvironment remains challenging. Here we developed an imaging pipeline using plus-end tip tracking and intravital microscopy to quantify MT dynamics in live xenograft tumor models. Among analyzed features, cancer cells in vivo displayed higher coherent orientation of MT dynamics along their cell major axes compared with 2D in vitro cultures, and distinct from 3D collagen gel cultures. This in vivo MT phenotype was reproduced in vitro when cells were co-cultured with IL4-polarized MΦ. MΦ depletion, MT disruption, targeted kinase inhibition, and altered MΦ polarization via IL10R blockade all reduced MT coherence and/or tumor cell elongation. We show that MT coherence is a defining feature for in vivo tumor cell dynamics and migration, modulated by local signaling from pro-tumor macrophages.

show abstract

Polarization of PI3K Activity Initiated by Ooplasmic Segregation Guides Nuclear Migration in the Mesendoderm

Cited by 11 publications

References 30 publications

Acquisition of the dorsal structures in chordate amphioxus

Acquisition of the dorsal structures in chordate amphioxus

Regulation and evolution of muscle development in tunicates

In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells

Contact Info

Product

Resources

About