Polarization-sensitive optical coherence elastography

Fukuda

et al. 2021

Sci Rep

Self Cite

We demonstrate label-free imaging of the functional and structural properties of microvascular complex in mice liver. The imaging was performed by a custom-built Jones-matrix based polarization sensitive optical coherence tomography (JM-OCT), which is capable of measuring tissue’s attenuation coefficient, birefringence, and tiny tissue dynamics. Two longitudinal studies comprising a healthy liver and an early fibrotic liver model were performed. In the healthy liver, we observed distinctive high dynamics beneath the vessel at the initial time point (0 h) and reappearance of high dynamics at 32-h time point. In the early fibrotic liver model, we observed high dynamics signal that reveals a clear network vascular structure by volume rendering. Longitudinal time-course imaging showed that these high dynamics signals faded and decreased over time.

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Label-free functional and structural imaging of liver microvascular complex in mice by Jones matrix optical coherence tomography

Mukherjee

Fukuda

et al. 2021

Sci Rep

Self Cite

“…Before performing PCA, the tissue surface was first segmented automatically, as described in Ref. 50 . An intensity threshold mask was then applied to remove the low signal region, and the region ranging from the tissue surface to a 100-pixel (724-µm) depth was then processed by PCA.…”

Section: Principal Component Analysis (Pca)mentioning

confidence: 99%

Label-free Functional and Structural Imaging of Liver Microvascular Complex in Mice by Jones Matrix Optical Coherence Tomography

Mukherjee

Fukuda

et al. 2021

Preprint

Self Cite

We demonstrate label-free imaging of the functional and structural properties of microvascular complex in mice liver. The imaging was performed by a custom-built Jones-matrix based polarization sensitive optical coherence tomography (JM-OCT), which is capable of measuring tissue’s attenuation coefficient, birefringence, and tiny tissue dynamics. Two longitudinal studies comprising a healthy liver and an early fibrotic liver model were performed. In the healthy liver, we observed distinctive high dynamics beneath the vessel at the initial time point (0 hour) and reappearance of high dynamics at 32-hour time point. In the early fibrotic liver model, we observed high dynamics signal that reveals a clear network vascular structure by volume rendering. Longitudinal time-course imaging showed that these high dynamics signals faded and decreased over time.

“…To calculate the AC, a polarization‐insensitive OCT intensity was computed at first. This polarization‐insensitive intensity is the average of the absolute‐squared intensities of four OCT signals corresponding to four polarization channels in Jones matrix 50 . The pixel‐by‐pixel depth‐resolved AC was determined using the method described by Vermeer et al 44 In this study, we did not correct the signal roll‐off by the limited coherence length of the light source and the signal attenuation caused by confocal gating.…”

Section: Methodsmentioning

confidence: 99%

Depth‐resolved investigation of multiple optical properties and wrinkle morphology in eye‐corner areas with multi‐contrast Jones matrix optical coherence tomography

Yamazaki

Skin Research and Technology

et al. 2020

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Background Multi‐contrast Jones matrix optical coherence tomography (JM‐OCT) can provide quantitative depth‐resolved local optical properties by improving the measurement algorithm. Materials and methods We examined the relationship between depth‐resolved local optical properties of eye‐corner skin measured by JM‐OCT and corresponding wrinkle morphology of aged women (n = 21; age range, 71.7 ± 1.7 years). Wrinkle morphology was analyzed by measuring the surface topography of three‐dimensional replicas. The same regions were measured three‐dimensionally by JM‐OCT, and the local optical properties at each depth were computed. Results Birefringence (BR) and mean wrinkle depth correlated significantly at a depth of 88.2‐138.6 µm from the skin surface, and attenuation coefficient (AC) and mean wrinkle depth correlated significantly at a depth of 12.6‐18.9 µm and 189‐459.9 μm from the skin surface, although a degree of polarization uniformity (DOPU) did not. Stepwise multiple regression analysis demonstrated that a significant regression equation (R2 = 0.649, P < .001) for predicting mean wrinkle depth was determined by BR at 107.1 µm depth (BR 107.1 µm), DOPU at 170.1 µm (DOPU 170.1µm), and AC at 252 µm (AC 252 µm) as independent variables and that these standardized beta regression coefficients were −0.860, −0.593, and −0.440, respectively, suggesting that BR, DOPU, and AC sufficiently explained mean wrinkle depth. Conclusion These results suggest that BR 107.1 µm, DOPU 170.1 µm, and AC 252 µm may indicate collagen‐related structure in the papillary, upper‐reticular dermis, and microstructure or tissue density in reticular dermis, respectively, and may be involved in wrinkle formation.