Polarized absorption, fluorescence and photoacoustic spectra of phycobilisomes embedded in poly(vinyl alcohol) films

Fra̧ckowiak, D.; Erokhina, L. G.; Balter, Aleksander; Lorrain, Lucie; Szurkowski, J.; Szych, B.

doi:10.1016/0005-2728(86)90123-4

Cited by 17 publications

(6 citation statements)

References 16 publications

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“…Linear dichroism values were positive in shorter and negative in longer wavelength regions for PE absorption, suggesting that PBS in PVA are not flattened by the film stretching. Therefore, on the basis of our absorption and L D results as well as previously published data (Frackowiak et al, 1986(Frackowiak et al, , 1987, we conclude that our PBS are rigid and oriented as shown in Fig. 2.…”

Section: Pbs Orientationsupporting

confidence: 88%

“…The maximal negative value reached 2%. A much higher degree of orientation was previously obtained for PBS from other organisms (Frackowiak et al, 1986(Frackowiak et al, , 1987 with the same magnitude of film stretching (4-fold). Tolypothrix PBS always showed a lower optical anisotropy compared with that from Nostoc (Frackowiak et al, 1987).…”

Section: Pbs Orientationsupporting

confidence: 54%

“…The polymer stretching generates the optical anisotropy of the embedded sample, but the mechanism responsible for this effect has not been clarified yet. Isolated chromophores and small complexes are oriented predomina;tly according to their shapes, with a tendency to be arranged with their longest axis parallel to the film-stretching direction (Norden, 1978), whereas large fragments of biological membranes are rather deformed as a result of film stretching (Lorrain et al, 1986). In the latter process, the optical anisotropy of the sample also occurs.…”

Section: Introductionmentioning

confidence: 99%

“…In the latter process, the optical anisotropy of the sample also occurs. Since PBS in cyanobacteria is an intermediate for this stretching effect (diameter, -50 nm; thickness, -30 nm) and is hemidiscoidal in shape (Gantt, 1981), it is not clear which process is mainly responsible for the observed linear dichroism (LD) of the absorption and photoacoustic spectra of PBS in PVA film (Frackowiak et al. 1986(Frackowiak et al.…”

Section: Introductionmentioning

confidence: 99%

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POLARIZED TIME‐RESOLVED FLUORESCENCE SPECTRA OF PHYCOBILISOMES ISOLATED FROM Tolypothrix tenuis EMBEDDED IN POLY(VINYL ALCOHOL) FILM

Fra̧ckowiak

Minuro

Yamazaki

et al. 1989

Photochem & Photobiology

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Time-resolved fluorescence spectra in the ps tiine range were measured on phycohilisome (PBS) emhedded in poly(viny1 alcohol) films. The cyanohacteriutn To!\.porhri.u renuis was used :I> a source of PBS because the pigment composition and the structure o f the PBS are well defined. Isotropic PBS in the unstretched film and PBS uniaxially oriented in the stretched film were investigated. Diameters of PBS heinidiscs were oriented parallel t o the film-stretching direction. The timeresolved fluorescence spectra of the unstretched sample and of the two polarized componcnts in the stretched samples showed several differences in the rise and decay. The delay time. estimated from the time span between the maximum laser pulse and maximal intensity of the phycocyanin and allophycocyanin fluorescence, was much longer than that reported in the aqueous media. This suggests occurrence of a higher thermal deactivation of PBS in polymer film than in aqueous media. The excitation energy transfer from excited phycocrythrin to allophycocyanin was more efficient in the unstretched than in the stretched samples, and it was greater in the parallel polarized component o f the stretched sample than in the perpendicular component. The present results are in agreement with a previous suggestion which states that there are two independent pathways of excitation energy transfer in PBS and that there is more than one final emitter of fluorescence. The molecules taking part in various pathways of energy transfer differ in their orientation within PBS.

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Section: Pbs Orientationsupporting

confidence: 88%

Section: Pbs Orientationsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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POLARIZED TIME‐RESOLVED FLUORESCENCE SPECTRA OF PHYCOBILISOMES ISOLATED FROM Tolypothrix tenuis EMBEDDED IN POLY(VINYL ALCOHOL) FILM

Fra̧ckowiak

Minuro

Yamazaki

et al. 1989

Photochem & Photobiology

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“…The efficiency of thermal deactivation (TD) of various separated biliproteins is different (Frackowiak et al, 1983b(Frackowiak et al, , 1985a. When they are included in PBS structure, the yield of TD is changed, because of excitation energy transfer between biliproteins (Frackowiak et al, 1986b).…”

Section: Absorption and Polarized Photoacoustic Spectra Of Anisotropimentioning

confidence: 99%

POLARIZED OPTICAL PROPERTIES OF PHYCOBILISOMES FROM Anabaena, Nostoc and Synechococcus

Fra̧ckowiak

Erokhina²,

Picard

et al. 1987

Photochem & Photobiology

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Polarized absorption, photoacoustic, fluorescence excitation and fluorescence emission spectra of phycobilisomes were measured when embedded in polyvinyl alcohol films. The phycobilisomes were isolated from the following organisms: Anabaena cylindrica, Nostoc punctiforma and Synechococcus elongatus. The ratio of photoacoustic spectra to absorption was taken as a measure of thermal dissipation of excitation energy. The isotropic samples and those oriented by the film stretching were investigated. The stretching of the sample strongly influences the efficiency of excitation energy transfer occurring among biliproteins in phycobilisomes, as is seen from the dramatic changes in the fluorescence and thermal dissipation spectra. The effect of stretching the film depends on the shape of phycobilisomes and on the strength of interactions between biliproteins in phycobilisomes.

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The Photoacoustic Method in Photosynthesis — Monitoring and Analysis of Phenomena Which Lead to Pressure Changes Following Light Excitation

Malkin

Biophysical Techniques in Photosynthesis

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Polarized absorption, fluorescence and photoacoustic spectra of phycobilisomes embedded in poly(vinyl alcohol) films

Cited by 17 publications

References 16 publications

POLARIZED TIME‐RESOLVED FLUORESCENCE SPECTRA OF PHYCOBILISOMES ISOLATED FROM Tolypothrix tenuis EMBEDDED IN POLY(VINYL ALCOHOL) FILM

POLARIZED TIME‐RESOLVED FLUORESCENCE SPECTRA OF PHYCOBILISOMES ISOLATED FROM Tolypothrix tenuis EMBEDDED IN POLY(VINYL ALCOHOL) FILM

POLARIZED OPTICAL PROPERTIES OF PHYCOBILISOMES FROM Anabaena, Nostoc and Synechococcus

The Photoacoustic Method in Photosynthesis — Monitoring and Analysis of Phenomena Which Lead to Pressure Changes Following Light Excitation

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