Polarized Expression of CD74 by Gastric Epithelial Cells

Barrera, Carlos A.; Beswick, Ellen J.; Sierra, Johanna C.; Bland, David A.; Espejo, Rosario; Mifflin, Randy C.; Adegboyega, Patrick A.; Crowe, Sheila E.; Ernst, Peter B.; Reyes, Victor E.

doi:10.1369/jhc.4a6552.2005

Cited by 25 publications

(22 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…GECs express CD74 and this expression increases during infection with H. pylori both in vivo and in vitro (24,34). CD74 was recently shown to be used by MIF as a receptor (22).…”

Section: Discussionmentioning

confidence: 99%

“…CD74 is known for its role in the regulation of intracellular transport and Ag-presenting functions of class II MHC molecules. Recent studies have shown that CD74 is not only expressed intracellularly, but also on a variety of cell surfaces, including GECs (23,24). We and others have shown that bound CD74 can induce signaling events leading to NF-B activation (25,26).…”

Section: Helicobacter Pylori Caga-dependent Macrophage Migration Inhimentioning

confidence: 92%

“…(22,33). We have shown that GECs express large amounts of CD74 (24,25), which is up-regulated during inflammatory conditions and H. pylori infection (34). Consequently, we examined the role of CD74 as a receptor for MIF on GECs by immunoprecipitation and Western blot analysis.…”

Section: H Pylori Induces Mif Production By Gecs In a Caga-dependentmentioning

confidence: 99%

See 2 more Smart Citations

Helicobacter pyloriCagA-Dependent Macrophage Migration Inhibitory Factor Produced by Gastric Epithelial Cells Binds to CD74 and Stimulates Procarcinogenic Events

Beswick

Pinchuk

Suárez

et al. 2006

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that has recently been implicated in carcinogenesis. Helicobacter pylori, which is closely linked to gastric cancer, induces the gastric epithelium to produce proinflammatory cytokines, including MIF. MIF can bind to CD74, which we have previously shown to be highly expressed on the surface of gastric epithelial cells (GEC) during H. pylori infection. In this study, we sought to investigate the role of the H. pylori-induced MIF on epithelial proliferation and procarcinogenic events. Upon establishing a role for the H. pylori CagA virulence factor in MIF production, MIF binding to CD74 on GEC was confirmed. rMIF and H. pylori were shown to increase GEC proliferation, which was decreased when cagA− strains were used and when CD74 was blocked by mAbs. Apoptosis was also decreased by MIF, but increased by cagA− strains that induced much lower amounts of MIF than the wild-type bacteria. Furthermore, MIF binding to CD74 was also shown to decrease p53 phosphorylation and up-regulate Bcl-2 expression. This data describes a novel system in which an H. pylori virulence factor contributes to the production of a host factor that in turn up-regulates procarcinogenic events by the gastric epithelium.

show abstract

“…GECs express CD74 and this expression increases during infection with H. pylori both in vivo and in vitro (24,34). CD74 was recently shown to be used by MIF as a receptor (22).…”

Section: Discussionmentioning

confidence: 99%

Section: Helicobacter Pylori Caga-dependent Macrophage Migration Inhimentioning

confidence: 92%

See 1 more Smart Citation

Helicobacter pyloriCagA-Dependent Macrophage Migration Inhibitory Factor Produced by Gastric Epithelial Cells Binds to CD74 and Stimulates Procarcinogenic Events

Beswick

Pinchuk

Suárez

et al. 2006

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…This notion is supported by studies on human mucosal immunity demonstrating that the Ii expressed in IEC plays an important role as potential regulators of local immune responses (Barrera et al, 1998;Hershberg and Mayer, 2000). Analogously, it has been reported that gastric epithelial cells and colon epithelial cells have a high expression level of surface Ii, which is closely correlated with anti-tumor immunity (Barrera et al, 2005;Tamori et al, 2005;Maharshak et al, 2010). IEC are located at a strategic position between the external environment and the most extended lymphoid tissue in the body.…”

Section: Tissue-specific Expression Of MDII Is Related To Structure Amentioning

confidence: 76%

Molecular characterization and tissue-specific expression of invariant chain in the muscovy duck (Cairina moschata)

Liu¹,

Chen²,

Dai³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The invariant chain (Ii) plays an important role as a chaperone for MHC II maturation and facilitates antigen presentation in vertebrates. We cloned, characterized and made a homology analysis of healthy adult muscovy duck Ii (MDIi), from a poultry farm in the suburban district of Hefei city in China, by rapid amplification of cDNA ends (RACE)-PCR and by measuring expression of the MDIi gene in various tissues by real-time quantitative PCR. A full-length cDNA sequence of MDIi was obtained, 1188-bp long, encoding a 222-amino acid protein. A comparison of the amino acid sequence of Ii between muscovy duck and other birds showed high similarity (66.3-95.3%). Characteristic functional domains found in Ii of other species, such as cytoplasmic domain, transmembrane domain, class ІІ-associated Ii-derived peptide (CLIP) and trimerization domain, were identified in MDIi. Although all functional domains of Ii were found to be highly conserved, small differences in the CLIP sequence occur among the various species. Expression of MDIi was detected in all tissues at different levels. A higher expression level was found in the spleen, intestinal mucosa and the bursa stipe (bursa of Fabricius stipe)

show abstract

“…A total of 50 ng cDNA was used to amplify HLA DM, CIITA, gapdh, and Ii from each cell type to confirm expression in stable cell lines, using the following primers: CD74 (Ii) (24); HLA-DM forward 59-ACTTTTCCCAGAACACTCGG-39, reverse 59-CTGGAAGC-TGAGTCCATCG-39; CIITA forward 59-AACCCGACACAGACACCAT-CAACT-39, reverse 59-GTCCAGTTCCGCGATATTGGCATA-39; gapdh forward 59-CAAAAGGGTCATCATCTCTGC-39, reverse 59-CAGGGGC-CATCCACAGTCTTC-39. Quantitative PCR (qPCR) was performed using the Bio-Rad iQ5, where 10-fold dilutions (500 ng to 500 pg) were used as template and expression of HIV-1 gag or CIITA was normalized to gapdh.…”

Section: Semiquantitative Pcr and Quantitative Pcrmentioning

confidence: 99%

CIITA Enhances HIV-1 Attachment to CD4+ T Cells Leading to Enhanced Infection and Cell Depletion

Porter¹,

Kelley²,

Nekorchuk³

et al. 2010

The Journal of Immunology

View full text Add to dashboard Cite

Activated CD4+ T cells are more susceptible to HIV infection than resting T cells; the reason for this remains unresolved. Induction of CIITA and subsequent expression of the MHC class II isotype HLA-DR are hallmarks of CD4+ T cell activation; therefore, we investigated the role of CIITA expression in T cells during HIV infection. CIITA-expressing SupT1 cells display enhanced virion attachment in a gp160/CD4-dependent manner, which results in increased HIV infection, virus release, and T cell depletion. Although increased attachment and infection of T cells correlated with HLA-DR surface expression, Ab blocking, transient expression of HLA-DR without CIITA, and short hairpin RNA knockdown demonstrate that HLA-DR does not directly enhance susceptibility of CIITA-expressing cells to HIV infection. Further analysis of the remaining MHC class II isotypes, HLA-DP and HLA-DQ, MHC class I isotypes, HLA-A, HLA-B, and HLA-C, and the class II Ag presentation genes, invariant chain and HLA-DM, demonstrate that these proteins likely do not contribute to CIITA enhancement of HIV infection. Finally, we demonstrate that in activated primary CD4+ T cells as HLA-DR/CIITA expression increases there is a corresponding increase in virion attachment. Overall, this work suggests that induction of CIITA expression upon CD4+ T cell activation contributes to enhanced attachment, infection, virus release, and cell death through an undefined CIITA transcription product that may serve as a new antiviral target.

show abstract

Polarized Expression of CD74 by Gastric Epithelial Cells

Cited by 25 publications

References 26 publications

Helicobacter pyloriCagA-Dependent Macrophage Migration Inhibitory Factor Produced by Gastric Epithelial Cells Binds to CD74 and Stimulates Procarcinogenic Events

Helicobacter pyloriCagA-Dependent Macrophage Migration Inhibitory Factor Produced by Gastric Epithelial Cells Binds to CD74 and Stimulates Procarcinogenic Events

Molecular characterization and tissue-specific expression of invariant chain in the muscovy duck (Cairina moschata)

CIITA Enhances HIV-1 Attachment to CD4+ T Cells Leading to Enhanced Infection and Cell Depletion

Contact Info

Product

Resources

About