Polarized infrared spectroscopy of oriented purple membrane

Rothschild, Kenneth J.; Clark, Noel A.

doi:10.1016/s0006-3495(79)85317-5

Cited by 235 publications

(220 citation statements)

References 30 publications

Supporting

Mentioning

194

Contrasting

Order By: Relevance

“…Dichroism measurements allowed us to calculate the average orientation of α-helices relative to the membrane plane. The result agreed well with the presence of an oriented bundle of α-helices consistent with the Unwin-Henderson electron diffraction derived 3D picture [201].…”

Section: The Discovery Of Oriented Transmembrane Alpha-helicessupporting

confidence: 86%

See 1 more Smart Citation

The early development and application of FTIR difference spectroscopy to membrane proteins: A personal perspective

Rothschild

2016

BSI

View full text Add to dashboard Cite

Abstract. Membrane proteins facilitate some of the most important cellular processes including energy conversion, ion transport and signal transduction. While conventional infrared absorption provides information about membrane protein secondary structure, a major challenge is to develop a dynamic picture of the functioning of membrane proteins at the molecular level. The introduction of FTIR difference spectroscopy around 1980 to study structural changes in membrane proteins along with a number of associated techniques including protein isotope labeling, site-directed mutagenesis, polarization dichroism, attenuated total reflection and time-resolved spectroscopy have led to significant progress towards this goal. It is now possible to routinely detect conformational changes of individual amino acid residues, backbone peptides, binding ligands, chromophores and even internal water molecules under physiological conditions with time-resolution down to nanoseconds. The advent of ultrafast pulsed-IR lasers has pushed this time-resolution down to femtoseconds. The early development of FTIR difference spectroscopy as applied to membrane proteins with special focus on bacteriorhodopsin is reviewed from a personal perspective.

show abstract

Section: The Discovery Of Oriented Transmembrane Alpha-helicessupporting

confidence: 86%

“…However, most membranes proteins do not produce such patches and are more difficult to incorporate into well order multilamellar films. For this purpose, we developed a method termed isopotential spin-drying (ISD) [57,201]. As shown in Fig.…”

Section: The Discovery Of Oriented Transmembrane Alpha-helicesmentioning

confidence: 99%

The early development and application of FTIR difference spectroscopy to membrane proteins: A personal perspective

Rothschild

2016

BSI

View full text Add to dashboard Cite

show abstract

“…Many of the peaks between 500 cm"1 and 3600 cm "1 in FTIR spectra of bR have been assigned to structural groups (Rothschild -and Clark, 1979a;Earnest et al, 1986). lt has been noted that bR's Amide A and Amide I peak frequencies are unusually high (Rothschild & Clark, 1979b;Krimm & Dwivedi, 1982;Glaeser et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

“…Nitrogen gas has been used to dry PM samples (Rothschild & Clark, 1979a). Clearly, little water, if any, remained after such drying (Rothschild & Clark, 1979a, see that it is unreasonable to expect that the thickness will be uniform.…”

Section: Concentrationmentioning

confidence: 99%

Trapping the M sub 1 and M sub 2 substrates of bacteriorhodopsin for electron diffraction studies

Perkins¹

1992

View full text Add to dashboard Cite

“…The dichroic order parameter arises from the distribution of angles, ϕ, made between the host director and the TDMs of the dyes, and may be expressed as a product of two contributing order parameters, given by Equation (4) [17], assuming uniaxial molecular alignment. In this expression, S θ is a molecular order parameter that is defined by the angle θ between the principal molecular axis of a dye molecule and the host director, where angular brackets denote an ensemble average.…”

Section: Introductionmentioning

confidence: 99%

Guest–host systems containing anthraquinone dyes with multiple visible transitions giving positive and negative dichroic order parameters: an assessment of principal molecular axes and computational methods

et al. 2017

View full text Add to dashboard Cite

(2017) Guest-host systems containing anthraquinone dyes with multiple visible transitions giving positive and negative dichroic order parameters: an assessment of principal molecular axes and computational methods. Liquid Crystals, Northumbria University has developed Northumbria Research Link (NRL) to enable users to access the University's research output. Copyright © and moral rights for items on NRL are retained by the individual author(s) and/or other copyright owners. Single copies of full items can be reproduced, displayed or performed, and given to third parties in any format or medium for personal research or study, educational, or not-for-profit purposes without prior permission or charge, provided the authors, title and full bibliographic details are given, as well as a hyperlink and/or URL to the original metadata page. The content must not be changed in any way. Full items must not be sold commercially in any format or medium without formal permission of the copyright holder. The full policy is available online: http://nrl.northumbria.ac.uk/policies.html This document may differ from the final, published version of the research and has been made available online in accordance with publisher policies. To read and/or cite from the published version of the research, please visit the publisher's website (a subscription may be required.)Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=tlct20 Three 1,4-disubstituted anthraquinone dyes with bis(4-n-butylphenyl) substituents connected via amine or amide linking groups have been studied as guest molecules dissolved in the nematic host E7. UV-visible absorption spectroscopy has shown each of the dyes to exhibit multiple absorption bands in the visible region, and dichroic order parameters obtained from polarised spectra of aligned guest-host samples were shown to differ significantly between the bands for each dye, and between the dyes. Time-dependent density functional theory calculations indicated that each dye exhibits several transitions, giving transition dipole moment vectors with a range of orientations, and fully atomistic molecular dynamics simulations of the guest-host mixtures showed differences in the calculated molecular alignments of the dyes. Combining the results from these two sets of calculations enabled a comparison of molecular alignment models based on the moments of inertia and the surface tensors of the dyes. The match between calculated and experimental values was improved significantly when using the surface tensor rather than the moment of inertia model, indicating that the shapes of the molecular surfaces of these dyes are crucial to their alignment. A novel method of calculating polarised UV-visible absorption spectra of dyes in liquid crystal hosts is also presented.

show abstract

Polarized infrared spectroscopy of oriented purple membrane

Cited by 235 publications

References 30 publications

The early development and application of FTIR difference spectroscopy to membrane proteins: A personal perspective

The early development and application of FTIR difference spectroscopy to membrane proteins: A personal perspective

Trapping the M sub 1 and M sub 2 substrates of bacteriorhodopsin for electron diffraction studies

Guest–host systems containing anthraquinone dyes with multiple visible transitions giving positive and negative dichroic order parameters: an assessment of principal molecular axes and computational methods

Contact Info

Product

Resources

About