Polarographic measurement of hydrogen sulfide production and consumption by mammalian tissues

Doeller, Jeannette E.; Isbell, T. Scott; Benavides, Gloria A.; Koenitzer, Jeffrey R.; Patel, H.; Patel, Rakesh P.; Lancaster, Jack R.; Darley‐Usmar, Victor; Kraus, David W.

doi:10.1016/j.ab.2005.03.024

Cited by 342 publications

(247 citation statements)

References 45 publications

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“…H 2 S is rapidly consumed in gills and gill mitochondria from sulfide-adapted mussel, Geukensia demissa, in normoxic conditions but reduced 50-fold by anoxia (79). In rat tissues, O 2 either inhibits H 2 S production or it may be associated with H 2 S consumption and both are reversed by hypoxia (30,44). Similar observations have been made in a variety of tissues from numerous vertebrates (32,89,94,126,128,186).…”

Section: Inverse Relationship Between O 2 and H 2 Ssupporting

confidence: 64%

Hydrogen sulfide as an oxygen sensor

Olson

2013

Clinical Chemistry and Laboratory Medicine

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Significance: Although oxygen (O 2 )-sensing cells and tissues have been known for decades, the identity of the O 2 -sensing mechanism has remained elusive. Evidence is accumulating that O 2 -dependent metabolism of hydrogen sulfide (H 2 S) is this enigmatic O 2 sensor. Recent Advances: The elucidation of biochemical pathways involved in H 2 S synthesis and metabolism have shown that reciprocal H 2 S/O 2 interactions have been inexorably linked throughout eukaryotic evolution; there are multiple foci by which O 2 controls H 2 S inactivation, and the effects of H 2 S on downstream signaling events are consistent with those activated by hypoxia. H 2 S-mediated O 2 sensing has been demonstrated in a variety of O 2 -sensing tissues in vertebrate cardiovascular and respiratory systems, including smooth muscle in systemic and respiratory blood vessels and airways, carotid body, adrenal medulla, and other peripheral as well as central chemoreceptors. Critical Issues: Information is now needed on the intracellular location and stoichometry of these signaling processes and how and which downstream effectors are activated by H 2 S and its metabolites. Future Directions: Development of specific inhibitors of H 2 S metabolism and effector activation as well as cellular organelle-targeted compounds that release H 2 S in a timeor environmentally controlled way will not only enhance our understanding of this signaling process but also provide direction for future therapeutic applications. Antioxid. Redox Signal. 22, 377-397.

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Section: Inverse Relationship Between O 2 and H 2 Ssupporting

confidence: 64%

Hydrogen sulfide as an oxygen sensor

Olson

2013

Clinical Chemistry and Laboratory Medicine

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“…NaHS was used in initial experiments whereas Na 2 S was used in later studies as it was more readily available and has fewer sulfur impurities (Doeller et al, 2005). Both salts form HS -and H 2 S when dissolved and we have not noticed any obvious differences in the effects produced by these salts .…”

Section: H 2 S Responsesmentioning

confidence: 99%

Hydrogen sulfide (H2S) and hypoxia inhibit salmonid gastrointestinal motility: evidence for H2S as an oxygen sensor

Dombkowski

Naylor

Shoemaker

et al. 2011

Journal of Experimental Biology

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SUMMARYHydrogen sulfide (H 2 S) has been shown to affect gastrointestinal (GI) motility and signaling in mammals and O 2 -dependent H 2 S metabolism has been proposed to serve as an O 2 ʻsensorʼ that couples hypoxic stimuli to effector responses in a variety of other O 2 -sensing tissues. The low P O2 values and high H 2 S concentrations routinely encountered in the GI tract suggest that H 2 S might also be involved in hypoxic responses in these tissues. In the present study we examined the effect of H 2 S on stomach, esophagus, gallbladder and intestinal motility in the rainbow trout (Oncorhynchus mykiss) and coho salmon (Oncorhynchus kisutch) and we evaluated the potential for H 2 S in oxygen sensing by examining GI responses to hypoxia in the presence of known inhibitors of H 2 S biosynthesis and by adding the sulfide donor cysteine (Cys). We also measured H 2 S production by intestinal tissue in real time and in the presence and absence of oxygen. In tissues exhibiting spontaneous contractions, H 2 S inhibited contraction magnitude (area under the curve and amplitude) and frequency, and in all tissues it reduced baseline tension in a concentration-dependent relationship. Longitudinal intestinal smooth muscle was significantly more sensitive to H 2 S than other tissues, exhibiting significant inhibitory responses at 1-10mmoll -1 H 2 S. The effects of hypoxia were essentially identical to those of H 2 S in longitudinal and circular intestinal smooth muscle; of special note was a unique transient stimulatory effect upon application of both hypoxia and H 2 S. Inhibitors of enzymes implicated in H 2 S biosynthesis (cystathionine -synthase and cystathionine -lyase) partially inhibited the effects of hypoxia whereas the hypoxic effects were augmented by the sulfide donor Cys. Furthermore, tissue production of H 2 S was inversely related to O 2 ; addition of Cys to intestinal tissue homogenate stimulated H 2 S production when the tissue was gassed with 100% nitrogen (~0% O 2 ), whereas addition of oxygen (~10% O 2 ) reversed this to net H 2 S consumption. This study shows that the inhibitory effects of H 2 S on the GI tract of a non-mammalian vertebrate are identical to those reported in mammals and they provide further evidence that H 2 S is a key mediator of the hypoxic response in a variety of O 2 -sensitive tissues.

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“…[77][78][79] Given the high reactivity of H 2 S in biological environments, traditional detection methods, e.g., colorimetric/electrochemical assays, gas chromatography, and sulfide precipitation, could yield inconsistent results due to lengthy and/or destructive procedures. [80][81][82][83] Consequently, the reported biologically relevant concentrations of H 2 S vary over a 10 5 -fold range, from 100 pM to 300 mM. 81,[84][85][86][87][88] Recent reports described several small-molecule fluorescent probes for H 2 S detection in living systems.…”

Section: Hydrogen Sulfide (H 2 S) Sensorsmentioning

confidence: 99%