Polarography of Anthraquinones and Dianthrones of Rhubarb

Asahi, Yutaka; Shinozaki, Kazuo; Mitani, Masayoshi; Ohtsuka, Hiroshi

doi:10.1248/cpb.22.254

Cited by 5 publications

(2 citation statements)

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“…The combined n-BuOH extracts were dried adequately on anhydrous Na2SO4, and nating electrolyte (T) was 0.01M valine adjusted to pH 9.9 by triethanolamine. A portion (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) that had been prepared to give same concentrations (g/ml) as in the above mentioned first MeOH-water extraction was injected, and the (1) the CITP system: The length of the sennoside A and B zones on the isotachopherograms of all the crude drug extracts examined (30, in total) were measured as the distances between the corresponding and next differential peaks, and then, the sennoside concentrations in individual specimens were calculated from the standard the above L solutions of 8 Rhei Rhyzoma and 2 Sennae Folium extracts was subjected to TLC on silica-gel G (Merck) using n-propanol/ethylacetate/water (4:4:3, v/v) as the developing solvent. After being developed, the plates were observed under an UV lamp (at 256 and 364 nm) to detect the fluorescent bands having the Rf 0.02 (sennoside B).…”

Section: Crude Drugs and Compoundsmentioning

confidence: 99%

Capillary-isotachophoretic detection and identification of sennosides A and B in extracts of crude plant drugs.

Hiraoka

Miura

1990

The physico-chemical biology Japanese journal of electrophoresi

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SUMMARYAn analytical system for separating sennosides A and B was established using capillaryisotachophoresis. These are the major purgative components of Rhei Rhyzoma (daiou; the rhyzomes of Rheim palmatum and related species) and Sennae Folium (Senna; the leaves of Cassia angusti florum and related species). Under the conditions employed, the zone of sennoside A was clearly separated from that of its stereoisomer, sennoside B, and the sennosides in the crude drug extracts were identified by mixed charging with authentic samples. The sennoside A and B contents in a total of 30 Rhei Rhyzoma and Sennae Folium specimens were then determined by this system, and a part of them (10 specimens, in total) was also treated by the thin-layer chromatographic method. The results showed that the present capillary-isotachophoresis system is powerful, at least to the same degree as the conventional thinlayer chromatographic system, as a tool for rapid and microscale analysis for use in pharmacognosy. It can be used to measure the major sennoside contents in a large number of crude drug specimens.

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Section: Crude Drugs and Compoundsmentioning

confidence: 99%

Capillary-isotachophoretic detection and identification of sennosides A and B in extracts of crude plant drugs.

Hiraoka

Miura

1990

The physico-chemical biology Japanese journal of electrophoresi

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“…Dianthrone (IX) 400 -404 ( Figure 35) and sennosides, 406 present in a few percent in rhubarb, show irreversible two-electron waves, which are ascribed to the cleavage of the C-C bridge to the anthrone (V) and reduction of the carbonyl to the corresponding alcohol [dianthranol (VIII)], which seems to be dehydrated to the corresponding 9,9'-dianthranyl (XI).…”

Section: Dianthrone (Ix) Dianthranol (Viii) Bianthrone (Vii) Anmentioning

confidence: 99%