Polarography of Proteins, Analytical Principles and Applications in Biological and Clinical Chemistry

Homolka, J

doi:10.1002/9780470110386.ch5

Cited by 10 publications

(2 citation statements)

References 90 publications

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“…Its application in medical diagnostics made the relatively simple »Brdi~ka reaction« or »Brdi~ka test« widely known and used. [15][16][17][18][19][20][21][22] Nickel ions produce similar effects, though much weaker, under the same conditions. 23,24 Later on, it was repeatedly shown that cations of other metals, 25,26 and in buffers other than ammoniacal, 27,28 cause catalytic evolution of hydrogen in the presence of certain ligands.…”

Section: »Brdi~ka« Catalysismentioning

confidence: 99%

Research Topic — Catalysis of Hydrogen Evolution on Mercury Electrodes

Heyrovský

2006

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Section: »Brdi~ka« Catalysismentioning

confidence: 99%

Research Topic — Catalysis of Hydrogen Evolution on Mercury Electrodes

Heyrovský

2006

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“…by Homolka [1971]. In the polarographic method, voltage and current in the test solution are constantly increased.…”

Section: Review On Methodology Of Determining Taamentioning

confidence: 99%

Thiol Amino Acids in Quality Control and Nutritive Value of Fishery Products

Ali¹

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Constant Current Chronopotentiometric Stripping Analysis of Bioactive Peptides at Mercury and Carbon Electrodes

et al. 1998

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Constant current derivative chronopotentiometric stripping analysis (CPSA) was used to study bioactive peptides [Lys 8 ]-vasopressin and angiotensin II at carbon paste electrode (CPE) and hanging mercury drop electrode (HMDE). Both peptides contain a single tyrosine residue which is oxidized at CPE close to þ0.6 V (against Ag/AgCl/3 M KCl electrode) producing peak Y; this peak was by about 15 mV more positive in vasopressin than in angiotensin II. At HMDE vasopressin yielded peak S due to the reduction of the disulfidic bond in the peptide. No peak S was observed with angiotensin II as it does not contain any cystine/cysteine residues. Vasopressin produced different catalytic hydrogen reduction signals in media with cobalt ion and in media not containing any metal ion. The signal produced in the latter media (peak H), which appeared close to ¹1.7 V, was well separated from the background. No catalytic hydrogen reduction signal was obtained with angiotensin II. The above mentioned CPSA signals can be applied for the determination of the respective peptide at nanomolar concentrations. The least sensitive peak S appears which requires at least 100 nM vasopressin concentration at moderate accumulation time. By two orders of magnitude, higher sensitivities can be obtained with peaks Y and H. In measuring these peaks CPSA shows significant advantages over the voltammetric stripping techniques.

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Polarography of Proteins, Analytical Principles and Applications in Biological and Clinical Chemistry

Cited by 10 publications

References 90 publications

Research Topic — Catalysis of Hydrogen Evolution on Mercury Electrodes

Research Topic — Catalysis of Hydrogen Evolution on Mercury Electrodes

Thiol Amino Acids in Quality Control and Nutritive Value of Fishery Products

Constant Current Chronopotentiometric Stripping Analysis of Bioactive Peptides at Mercury and Carbon Electrodes

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