The purpose of this research was to investigate the relationship between job satisfaction and psychological well-being levels of different occupational employees (education, security, health, justice, worker, engineer, and religious official) carrying on their duties in different institutions and organizations in a mid-scale provincial center of Eastern Anatolian region in Turkey. Furthermore, the research also discussed the differentiation between psychological well-being and job satisfaction in terms of occupational areas, income levels and service period of different occupational employees. The research group included totally 348 employees including 107 female and 241 males between 21 and 64 years old. Psychological Well-Being Scale, Job Satisfaction Scale, and Personal Information Form were used as data collection tools in the research. According to the research results, a positive mid-level relationship was proved between psychological well-being levels and job satisfaction levels of the employees. In terms of occupational areas and income levels, a significant differentiation was observed between psychological well-being and job satisfaction scores of the occupational employees. Finally, no significant difference was determined between psychological well-being and job satisfaction levels of the employees.