Polish Yellow Sweet Clover (Melilotus officinalis L.) Honey, Chromatographic Fingerprints, and Chemical Markers

Jasicka-Misiak, Izabela; Makowicz, Ewa; Stanek, Natalia

doi:10.3390/molecules22010138

Cited by 30 publications

(27 citation statements)

References 32 publications

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“…It is believed that Melilotus honey contains coumarin compounds, characteristic secondary metabolites found in the Melilotus flowers and leaves. However, there are few studies on the chemical composition of Melilot honey, especially in terms of coumarin content [8,9]. In our previous study, for the first time, we proved that Melilotus albus honey exhibits antioxidant and antimicrobial activity [10].…”

Section: Introductionmentioning

confidence: 71%

A novel honey-based product enriched with coumarin from Melilotus flowers

et al. 2019

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Coumarin is a plant secondary metabolite responsible for the health-promoting properties of the Melilotus officinalis herb used in traditional medicine in the treatment of lymphedema and chronic venous diseases. The aim of this study was to analyze the possibility of honey enrichment with coumarin derived from sweet clover (Melilotus) in two different ways: herb honey and herb-infused honey production. Herb honey was obtained by feeding bees with sugar syrup enriched with a M. officinalis herb water infusion, whereas herbal macerates in multifloral honey were prepared with crushed flowers (fresh and dried) of M. officinalis and Melilotus albus at 20 °C in dark place for 6 months. Melilotus albus nectar honey (n = 14) was used as control. Using HPLC analysis, the highest content of coumarin (from 41.33 to 137.89 mg/kg) and its precursor o-coumaric acid (from 6.57 to 25.15 mg/kg) in M. officinalis flowers-infused honey were found. Herb honey contains 4.48 mg/kg coumarin and for the first time, this compound was determined in nectar honey from Polish M. albus honey (0.05 to 0.88 mg/kg). The addition of Melilotus flowers enhanced antioxidant activity (measured by FRAP, DPPH and PCL methods) of infused-honey in comparison to multifloral honey (control) and herb honey. The coumarin and o-coumaric acid were weakly correlated with antioxidant activity. It was found that only herb-infused honey can be proposed as the natural safe remedy for venous diseases prevention.

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Section: Introductionmentioning

confidence: 71%

A novel honey-based product enriched with coumarin from Melilotus flowers

et al. 2019

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“…However, recent study by Jasicka‐Misiak et al . () not confirmed the presence of this compounds in fresh M. officinalis honey.…”

Section: Introductionmentioning

confidence: 82%

Hydrogen peroxide-dependent antibacterial action of Melilotus albus honey

Sowa

Grabek-Lejko

Wesołowska

et al. 2017

Lett Appl Microbiol

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Melilot honey is used in traditional medicine as an anticoagulant agent due to the possibility of the presence of the coumarin compounds which are specific for Melilotus plant. Melilotus albus is rarely used to produce honey, and antibacterial properties of this variety of honey had not been studied yet. Nine samples of melilot honey produced in different regions of Poland were analysed according to their antibacterial activity which was correlated with physiochemical parameters and antioxidant activity. It was shown that antibacterial activity of melilot honey is created by hydrogen peroxide and other bioactive compounds.

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“…The extraction was performed using the method described by Jasicka-Misiak et al (2017). The honey (25 g) was thoroughly mixed with five parts (125 mL) of acidified water (pH = 2 with HCl) and homogenized in an ultrasonic bath for 60 min.…”

Section: Phenolic Extraction (Solid-phase Extraction Spe)mentioning

confidence: 99%

HPTLC Phenolic Profiles as Useful Tools for the Authentication of Honey

Stanek

Jasicka-Misiak

2018

Food Anal. Methods

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The present study reveals the utility of high-performance thin-layer chromatographic (HPTLC) fingerprinting of phenolic constituents for the authentication of monofloral honeys. The obtained data enables a more complete assessment of honey quality and the identification of emerging threats to honey quality. The developed procedure facilitates differentiation of varietal honeys and detection of honey adulterations. We used an HPTLC fingerprint analysis to determine the characteristic patterns of different honey types (willow, buckwheat, heather, pine honeydew, and manuka honey). The HPTLC chromatograms were used to determine the differences in the botanical origin of the honey samples on the basis of the band profiles, which are characteristic for each honey type. Identification of 11 polyphenols was performed by comparison of the color and R f of the bands with available standards. Additionally, the results were confirmed by an HPLC analysis.

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Polish Yellow Sweet Clover (Melilotus officinalis L.) Honey, Chromatographic Fingerprints, and Chemical Markers

Cited by 30 publications

References 32 publications

A novel honey-based product enriched with coumarin from Melilotus flowers

A novel honey-based product enriched with coumarin from Melilotus flowers

Hydrogen peroxide-dependent antibacterial action of Melilotus albus honey

HPTLC Phenolic Profiles as Useful Tools for the Authentication of Honey

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