With the rapid development of China’s economy, the demand for water resources continues to sharply increase, which has gradually contributed to serious environmental problems. The Chinese government has proposed establishing a natural resource balance sheet, which is expected to solve this problem by assessing the value of water resources. The main purpose of this study was to assess the value of water resources in Wuhan from 2013 to 2017. Based on a fuzzy mathematical evaluation model, 15 indicators were chosen considering the three main aspects of resources, society, and the environment to construct a water resource evaluation indicator system. In addition, the analytic hierarchy process (AHP) and entropy weight methods were combined to determine the index weight. Based on this, we calculated the value of water resources in Wuhan from 2013 to 2017. The results demonstrated that the values of water resources in Wuhan from 2013 to 2017 were US$2.910 billion, US$5.006 billion, US$9.223 billion, US$14.167 billion, and US$7.189 billion, respectively. Therefore, this paper provides a scientific foundation for the rational establishment of water prices, the assessment of local natural resource assets, and the preparation of natural resource balance sheets.