Pollen- and anther-specific chi promoters from petunia: tandem promoter regulation of the chiA gene.

Tunen, Arjen J. van; Mur, Luuc R.; Brouns, Gaby; Rienstra, J. D.; Koes, Ronald; Mol, J.A.

doi:10.1105/tpc.2.5.393

Cited by 87 publications

(39 citation statements)

References 18 publications

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“…These experiments complement our previous results Goldberg, 1980, 1984) and those of others (Stinson et al, 1987;Gasser et al, 1988;Ursin et al, 1989;Twell et al, 1990;van Tunen et al, 1990) and indicate that the differentiated state of the anther is associated with the expression of unique genes. A major aspect of the results presented here is the finding that different temporal and spatial gene expression programs occur during anther development and that these programs correlate with the differentiation of specific tissues and cell types.…”

Section: Gene Expression Is Temporally and Spatially Regulated Duringsupporting

confidence: 80%

Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development.

et al. 1990

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We studied the temporal and spatial regulation of three mRNA sequence sets that are present exclusively, or at elevated levels, in the tobacco anther. One mRNA set accumulates in the tapetum and decays as the tapetum degenerates later in anther development. The second mRNA set accumulates after the tapetal-specific mRNAs, is localized within the stomium and connective, and also decays as these cell types degenerate during anther maturation. The third mRNA sequence set persists throughout anther development and is localized within most anther tissues. A tapetal-specific gene, designated as TA29, was isolated from a tobacco genome library. Runoff transcription studies and experiments with chimeric p-glucuronidase and diphtheria toxin A-chain genes showed that the TA29 gene is regulated primarily at the transcriptional leve1 and that a 122-base pair 5' region can program the tapetal-specific expression pattern. Destruction of the tapetum by the cytotoxic gene had no effect on the differentiation and/or function of surrounding sporophytic tissues but led to the production of male-sterile plants. Together, our studies show that several independent gene expression programs occur during anther development and that these programs correlate with the differentiated state of specific anther cell types.

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Section: Gene Expression Is Temporally and Spatially Regulated Duringsupporting

confidence: 80%

Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development.

et al. 1990

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“…The tissues were ground in liquid N 2 in the presence of Dowex-1 (Sigma) to remove flavonoids (Van Tunen et al, 1990). For each flower, the GUS activity of the limb, which was normalized to the protein concentration, was divided by that of the sepal (C/S ratio).…”

Section: Fluorometric Gus Assay and Statistical Analysismentioning

confidence: 99%

Post‐transcriptional silencing of chalcone synthase in Petunia by inverted transgene repeats

et al. 1997

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SummaryTo induce post.transcriptional silencing of flower pigmentation genes by homologous sense transgenes in transgenic petunias, it is not necessary for the transgenes to be highly transcribed. Even promoterless transgenes can induce silencing. Here it is shown that in these cases silencing is mediated by multimeric transgene/T-DNA loci in which the T-DNAs are arranged as inverted repeats (IRs). With the transgene constructs used, monomeric T-DNA loci are unable to confer silencing even though they modulate IR-induced silencing. IRs with the silencing sequences proximal to the centre (IR c) induce a more severe silencing than IRs with these sequences distal to the centre (IR,). Somatic reversion of silencing, as observed in a side branch of one of the chalcone synthase (Chs) transformants, was associated with a deletion of the IR locus from L1 cells, the meristematic cell layer that expresses the endogenous Chs genes in the flower corolla. Taken together, these data indicate that the post-transcriptional silencing mechanism can be activated by inverted transgene repeats. It is also shown that a silent IR UidA-ChsA locus silences the expression of a monomeric 35S promoter-driven UidA-ChsA transgene only in corollas where the endogenous Chs genes are highly transcribed. These results are consistent with a model in which an IR, by virtue of its palindromic sequence organization, is able to promote the production of aberrant RNAs from the endogenous homologs as a result of ectopic pairing.

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“…In addition to the LAT genes, the upstream regions of two other genes that are similarly regulated during pollen development have been sequenced: the maize Zmgl3 gene {Hamilton et al 1989}, the coding region of which shares sequence similarity with that of LAT52, and the petunia chalcone flavone isomerase [chi) A gene (van Tunen et al 1989), the upstream promoter (chiA PAX) of which is sufficient to direct pollen-specific expression in petunia (van Tunen et al 1990). In the Zmgl3 promoter, five sequences were found (at positions -495 bp, -439 bp, -170 bp, -141 bp, and -49 bp) that contained a GTGG motif and showed at least five of seven matches to the PB core motif.…”

Section: Conservation Of Lat Gene Regulatory Elements In Other Pollenmentioning

confidence: 99%

Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements.

Twell

Yamaguchi²,

Wing³

et al. 1991

Genes Dev.

292

220

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We have investigated the functional organization and properties of cis regulatory elements in the promoter regions of two genes from tomato (LAT52 and LAT59) that are preferentially and coordinately expressed during pollen maturation. Promoter deletion analysis in transgenic plants demonstrated that only minimal (~200 bp) promoter proximal regions are required for developmentally regulated expression in pollen and in specific cell types of the sporophyte. Cis-acting regulatory regions of these two promoters and of a third pollen-expressed promoter (LAT56) were characterized in detail using a transient expression assay. We identified two upstream activator regions in the LAT52 promoter and further showed that a 19-bp segment from one of those regions enhanced expression of the heterologous CaMV35S promoter in pollen. Similarities in sequence between crucial cis elements provide evidence that shared regulatory elements are involved in the coordinate expression of the LAT genes during microsporogenesis.[Key Words: Tomato; tobacco; male gametophyte; transcriptional control; enhancer; cell-specific gene regulation]Received September 28, 1990; revised version accepted January 4, 1991.Identifying the molecular components that control the spatial and temporal patterns of gene expression accompanying gametogenesis is an important step in understanding sexual reproduction in eukaryotes. We are interested in the development of the male gametophyte (pollen) of angiosperms because it provides a unique system with which to study cell-specific gene regulation and cellular differentiation during reproductive development in plants. Major cytological and biochemical events that occur during pollen development are well characterized, the mRNA populations in pollen have been analyzed, pollen-specific isoenzymes have been identified, and several genes that are preferentially expressed in pollen in a stage-specific manner recently have been cloned and sequenced (for reviews, see Mascarenhas 1975Mascarenhas , 1989. Of particular interest is the asymmetric mitotic division of the haploid microspore (microspore mitosis), which results in the formation of two dimorphic cells with different developmental fates. The Present addresses: ~Leicester Biocentre, University of Leicester, Leicester LE1 7RH UK;

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Pollen- and anther-specific chi promoters from petunia: tandem promoter regulation of the chiA gene.

Cited by 87 publications

References 18 publications

Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development.

Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development.

Post‐transcriptional silencing of chalcone synthase in Petunia by inverted transgene repeats

Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements.

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