Pollen Characteristics and Genetics of Induced and Spontaneous Genetic Male‐Sterile Mutants in Rice

Hu, Jinguo; Rutger, J. N.

doi:10.1111/j.1439-0523.1992.tb00159.x

Cited by 12 publications

(9 citation statements)

References 11 publications

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“…For example, there are more than 60 genes that affect male fertility in maize or rice (Albertsen and Palmer 1979;Albertsen and Phillips 1981;Kaul1988;Hu and Rutger 1992). Recent effords using Arabidopsis thaliana have led to the cloning and molecular characterization of a number of male sterility genes.…”

Section: A) Anther Development and Nuclear Male Sterilitymentioning

confidence: 99%

Plant Breeding: Male Sterility in Higher Plants - Fundamentals and Applications

Kempken

Pring

1999

Progress in Botany

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Section: A) Anther Development and Nuclear Male Sterilitymentioning

confidence: 99%

Plant Breeding: Male Sterility in Higher Plants - Fundamentals and Applications

Kempken

Pring

1999

Progress in Botany

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“…Thus, breeders are interested in finding a mutagenic dose that induces adequate mutagenic outcome but which results in low sterility and lethality. Efficiency of the LD 50 criterion has been validated by almost all researchers (Das and Haque, 1997;Gustafson, 1989;Hu and Rutger, 1992;Snustad and Simmons, 2006). According to this criterion, treatment with 0.8% EMS solution for 6 h has led to 50% lethality compared to that of control (Table 3).…”

Section: Discussionmentioning

confidence: 97%

“…Mutagen dose, treatment period and their interaction can be considered as the main factors also influenced by pretreatment, temperature, pH, and post-treatment (Hu and Rutger, 1992). Lethal dose 50 (LD 50 ) is generally used as a criterion to define the optimum mutagenic dose.…”

Section: Introductionmentioning

confidence: 99%

Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens

Emrani¹,

Arzani²,

Saeidi³

2011

Afr. J. Biotechnol.

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Mutation induction is considered as an effective way to enrich plant genetic variation, particularly for traits with a very low level of genetic variation. The objectives of this study were to evaluate the effect of different dosages of chemical mutagens on seed germination, seed viability and seedling growth characteristics and to identify optimum treatment conditions for chemical mutagens based on the LD 50 criterion in canola (Brassica napus L.). Two pretreatment conditions of soaking in distilled water and non-soaking, different concentrations of chemical mutagens, and four treatment periods were investigated. The effect of mutagen dosage on seed viability was also assessed using the tetrazolium staining test. Results revealed the significant effects of mutagen dosages and treatment periods on seed viability and seed germination as well as on seedling characteristics for all the mutagens tested. Additionally, it was found that increased dosage and period in each treatment led to significant reductions in seed viability for the tested mutagens. Pretreatment did not significantly influence most of the studied characteristics. The 0.8% ethyl methanesulfonate (EMS) for 6 h, 12 mM N-nitroso-Nethylurea (ENU) and 6 mM sodium azide for 8 h and 9 mM N-nitroso-N-methylurea (NMU) for 4 h were considered as optimum treatment conditions.

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“…For each treatment, 150 g of Katy rice was used. EMS treatment was as described in Hu and Rutger [19] and performed at USDA ARS Dale Bumpers National Rice Research Center in 2001. Specifically, approximately 7500 seeds of Katy were treated with 0.8% (v/v) EMS in 2001.…”

Section: Mutagen Treatmentmentioning

confidence: 99%

Development and Characterization of a Large Mutant Population of a Rice Variety Katy for Functional Genomics Studies and Breeding

2019

Crop Breed Genet Genom.

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The availability of a large mutant population is an important genetic resource for functional genomic studies and breeding. Katy, a publicly available long grain tropical japonica rice variety with an excellent package of disease resistance and suitable agronomic traits, was used to develop a large mutant population. A combination of ethyl methane sulfonate (EMS), fast neutron (FN), and gamma irradiation ( 60 Co) treatments were applied at varying dosages to develop the population. Approximately 7500 rice seeds were each treated with EMS (0.4%, 0.8%, and 1.2%) and 60 Co (200 Gy), and approximately 10,000 rice seeds were exposed to 7.7 Gy, 26.3 Gy, and 49.4 Gy dosages of FN. Mutation effects were initially evaluated at the M 2 generation by assessing chlorophyll biosynthesis deficiencies. The mutation effects of each line were detected at 1.04% for 0.4% EMS, 5.04% for 0.8% and 1.2% EMS, 2.9% for 26.3 Gy FN, 3.2% for 49.4 Gy FN, and 5.04% for 60 Co 200 Gy for the M 2 population. After seeds were advanced to M 4 using a single seed breeding strategy, the genotype identity of 189 randomly selected lines was verified with nine simple sequence repeat markers and 96 randomly selected lines were evaluated for mutant morphological phenotypes. The analysis uncovered slightly higher frequencies of morphological mutants at M 4 than was observed in the M 2 generation. These findings suggest that the Katy putative mutant population consisting of 23,558 individuals is a potential asset for rice functional genomics studies and breeding.

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Pollen Characteristics and Genetics of Induced and Spontaneous Genetic Male‐Sterile Mutants in Rice

Cited by 12 publications

References 11 publications

Plant Breeding: Male Sterility in Higher Plants - Fundamentals and Applications

Plant Breeding: Male Sterility in Higher Plants - Fundamentals and Applications

Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens

Development and Characterization of a Large Mutant Population of a Rice Variety Katy for Functional Genomics Studies and Breeding

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