Pollen extraction from insects

Jones, G. D.

doi:10.1080/01916122.2011.629523

Cited by 38 publications

(27 citation statements)

References 69 publications

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“…Standard pollen processing methods are described in Faegri et al (1989) and previous processing methods for pollen derived from insects are in Jones (2012Jones ( , 2014. In contrast, I…”

Section: Pollen Extractionmentioning

confidence: 99%

“…Palynology (pollen analysis) is a powerful tool for determining plant-pollinator or plantpalynivore relationships when direct observation of floral visits is not possible. Pollen grains can be recovered from the insect cuticle and setae (Jones and Jones 2001, Jones 2012, Wappler et al 2015, from the guts of palynivorous insects (Jones 2012), and the rectum of honey bees (Hymenoptera: Apidae: Apis mellifera L.; Dimou and Thrasyvoulou 2009). Previous investigations have also recovered pollen from beetle frass (Davidson and Evans 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Acetolysis leaves the ectexine, or outermost stratum of the pollen grain, intact (Jones 2014). The ectexine remains even as the cytoplasm is digested in the gut of palynivorous insects (Jones 2012). For this reason, the remains of pollen material can be easily identified in insect frass.…”

Section: Introductionmentioning

confidence: 99%

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Identifying floral hosts of cerambycid beetles using palynology

Hazel¹

2016

2016 International Congress of Entomology

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Pollen is an important food source for many insects including the adults of beetles whose larvae are important forest pests. I quantified the distribution of pollen-feeding in adults of several species of long-horned beetles (Coleoptera: Cerambycidae). Palynivory by cerambycid beetles is common in the subfamily Lepturinae and occurs sporadically in Cerambycinae. The current knowledge of feeding in this group is inferred from floral host records, which are biased against tall trees because they are inaccessible to naturalists. I present a simplified palynological method for extracting and quantifying pollen from the frass of 21 species of beetles collected on flowers, with pheromone-baited panel traps, and at lights. I identified pollen grains to genus or species when possible and estimated abundances using calculations based on standardized markers added to samples. I found a higher abundance of pollen grains in the frass of palynivorous beetles collected on flowers than in the frass of beetles collected otherwise. I used the Shannon-Wiener Index to describe the diversity of pollen in the frass of beetles collected from flowers. There was high alpha diversity of pollen grains in the frass of Callimoxys sanguinicollis (Olivier) on Cornus drummondii C.A.Mey. (Cornaceae) and Molorchus bimaculatus Say on Cornus florida L. Next, I described the pollen taxa in the frass of palynivorous beetles collecting in traps and at lights by calculating the mean abundance and proportions of pollen taxa. I found Juglans nigra L. (Juglandaceae), Quercus L. (Fagaceae), and Vitis L. (Vitaceae) in frass from Anelaphus pumilus (Newman). Acer L. (Sapindaceae), Viburnum L. (Caprifoliaceae), and Quercus was found in frass from Cyrtophorus verrucosus (Olivier). I recorded Acer and Quercus pollen in the frass of Euderces pini (Olivier). Megacyllene caryae (Gahan) frass contained Quercus and Crataegus Tourn. (Rosaceae) pollen. Finally, the abundance, richness, and composition of pollen grains in the frass of Megacyllene caryae varied with collection date (e.g., April 17 vs. April 25, 2016) and location (e.g., forest vs. urban sites). These results provide insight into the natural history and feeding behavior of longhorned beetles, potentially influencing conservation or pest management strategies for forest insects. Moreover, I provide a method to secure evidence of palynivory in insects without having to carry out dissections or field observations.

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“…Standard pollen processing methods are described in Faegri et al (1989) and previous processing methods for pollen derived from insects are in Jones (2012Jones ( , 2014. In contrast, I…”

Section: Pollen Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identifying floral hosts of cerambycid beetles using palynology

Hazel¹

2016

2016 International Congress of Entomology

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“…Safranin O is the preferred stain for most uses in palynology, staining the pollen grains pink to red depending on the amount of stain and type of pollen analysed (Jones, 2012).…”

Section: Heat the Solution Gently To Boiling Point In A Water Bathmentioning

confidence: 99%

Standard methods for pollination research withApis mellifera

Delaplane¹,

Dag

Danka

et al. 2013

Journal of Apicultural Research

235

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SummaryIn this chapter we present a synthesis of recommendations for conducting field experiments with honey bees in the context of agricultural pollination. We begin with an overview of methods for determining the mating system requirements of plants and the efficacy of specific pollinators. We describe methods for evaluating the pollen-vectoring capacity of bees at the level of individuals or colonies and follow with methods for determining optimum colony field stocking densities. We include sections for determining post-harvest effects of pollination, the effects of colony management (including glasshouse enclosure) on bee pollination performance, and a brief section on considerations about pesticides and their impact on pollinator performance. A final section gives guidance on determining the economic valuation of honey bee colony inputs at the scale of the farm or region. Métodos estándar para el estudio de polinización con Apis mellifera ResumenEn este capítulo se presenta una síntesis de las recomendaciones para la realización de experimentos de campo con abejas melíferas en el contexto de la polinización agrícola. Comienza con una revisión de los métodos para la determinación de los requisitos del sistema de reproducción de las plantas y de la eficacia de los polinizadores específicos. Se describen métodos para evaluar la capacidad de las abejas como vectores de polen a los niveles de individuos o de colonias, y se continúa con los métodos para la determinación de las densidades óptimas de colonias en campo. Se incluyen secciones para la determinación de los efectos de la polinización en la cosecha, los efectos del manejo de las colonias (incluyendo el cercado en invernaderos) en el rendimiento de polinización de las abejas, y una breve sección sobre consideraciones acerca de los plaguicidas y su impacto en el rendimiento de los polinizadores. Una última sección ofrece una guía para la determinación del valor económico de los gastos de las colonias de abejas melíferas a escala de explotación o de región.

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“…To increase the pollen diversity and the number of recovered pollen grains, the whole insect was processed using light microscopy (Jones, 2012). Each boll weevil or all boll weevil parts were put into a uniquely marked 1.5 ml micro-centrifuge tube.…”

Section: Processingmentioning

confidence: 99%

Pollen extraction from insects

Cited by 38 publications

References 69 publications

Identifying floral hosts of cerambycid beetles using palynology

Identifying floral hosts of cerambycid beetles using palynology

Standard methods for pollination research withApis mellifera

Forensic pollen geolocation techniques used to identify the origin of boll weevil re-infestation

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