Pollen Fertility Assessment Through Acetocarmine Staining and in Vitro Germination in Solanum Tuberosum L.

Abstract

The wide occurrence of potato genotypes characterized by male sterility considerably complicates the realization of breeding programs. Therefore, male fertility in potato germplasm requires assessment as an important task. In the fresh study, 57 breeding lines from the Russian Potato Research Center and 85 cultivars of different origins underwent acetocarmine staining and in vitro germination assessment. With acetocarmine staining, 22 samples exhibited with tetrad sterility. In the remaining 120 samples, stained pollens vary from 0% to 88.7%, and germination varies from 0% to 19.4%. The pollen fertility assessment data obtained through acetocarmine staining and in vitro germination differed significantly. In the studied potato accessions, acetocarmine staining provided 18.3% sterile samples, while 50.7% by in vitro germination. The highest stained pollens (>80%) appeared in five potato accessions, i.e., 3004-7 (88.7%), 3000-32 (86.5%), 21.32-1 (81.8%), 17.32-1 (80.3%), and cultivar Garant (86.8%). In most potato accessions, the colored pollen grains ranged from 40% to 70%. The maximum percent of pollen germination occurred in the sample, 21.32-1 (19.4%). However, seven accessions (21.32-1, 18.5-17, 20.3-6, 20.33-8, and cultivars Edison, Queen Anne, and Garant) showed more than 10% pollen germination. During the growing season of 2022, the weather was hotter and drier compared with past long-term data, which could affect a decrease in the ability of pollens to germinate. The obtained results on male fertility will help in the study of the genetic control of pollen fertility and a further improvement in the breeding of Solanum tuberosum L.

Plant Segregation and Pollen Characteristics of Highland Papaya (Vasconcellea Pubescens A.DC.) Based on Sex Types

“…Pollen germination indicated pollen viability, while pollen tube length showed vigor (Sulusoglu and Cavusoglu, 2014). Pollen germination is one of the methods used to assess pollen fertility, previously carried out on 142 accessions of Solanum tuberosum L. (Gins et al, 2022). For male dioecious flowers of V. pubescens, the pollen germination was faster than in monoecious plants, indicating the need for male plants in the cultivation.…”

Sulfuric Acid and Hot Water Treatment Effects on the Seed Germination and Growth Traits of Sesbania Punicea L.

“…Sesbania is a nitrogen-fixing shrub that can restore disturbed soils by improving soil nitrogen (N2) and fertility via the nodulation process between bacterial nodules and sisban roots and can also serve as fodder and wood fuel (Grzeça et al, 2021). Sesban species propagation is often by the seeds and represents natural resources for reproduction and maintenance of genetic diversity in crop plants (Taiz and Zeiger, 2002;Gins et al, 2022;Narimonov et al, 2023).…”

Genetic Variability, Heritability, and Genetic Gain in Sweet Potato (Ipomoea Batatas L. Lam) for Agronomic Traits

“…The importance of sweet potato as a food crop flourishes rapidly in some parts of the world. It is also a good food-security crop and sells for cash by poor resource farmers (Tairo and Kullaya, 2004;Dalamu et al, 2012;Rawaida and Nur-Fazliana, 2019;Haq et al, 2021;Gins et al, 2022).…”