Pollen from Cry1Ac/CpTI-transgenic cotton does not affect the pollinating beetle Haptoncus luteolus

Chen, Lizhen; Cui, Jinjie; Ma, Weihua; Niu, Changying; Lei, Chaoliang

doi:10.1007/s10340-010-0319-0

Cited by 19 publications

(7 citation statements)

References 34 publications

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“…The level of the Cry2Ab toxin, however, was stable. Recent studies conducted on another GM cotton cultivar also demonstrated temporal variances in Cry1Ac protein expression [18], [25], [42].…”

Section: Discussionmentioning

confidence: 89%

Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Niu

Mannakkara

et al. 2013

PLoS ONE

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Transgenic insect-resistant cotton (Bt cotton) has been extensively planted in China, but its effects on non-targeted insect species such as the economically important honey bee (Apis mellifera) and silkworm (Bombyx mori) currently are unknown. In this study, pollen from two Bt cotton cultivars, one expressing Cry1Ac/EPSPS and the other expressing Cry1Ac/Cry2Ab, were used to evaluate the effects of Bt cotton on adult honey bees and silkworm larvae. Laboratory feeding studies showed no adverse effects on the survival, cumulative consumption, and total hemocyte count (THC) of A. mellifera fed with Bt pollen for 7 days. No effects on the survival or development of B. mori larvae were observed either. A marginally significant difference between Cry1Ac/Cry2Ab cotton and the conventional cotton on the THC of the 3rd day of 5th B. mori instar larvae was observed only at the two highest pollen densities (approximately 900 and 8000 grains/cm2), which are much higher than the pollen deposition that occurs under normal field conditions. The results of this study show that pollen of the tested Bt cotton varieties carried no lethal or sublethal risk for A. mellifera, and the risk for B. mori was negligible.

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Section: Discussionmentioning

confidence: 89%

Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Niu

Mannakkara

et al. 2013

PLoS ONE

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“…Pollen from Cry1Ac+CpTI transgenic cotton (CCRI41) did not affect the pollinating beetle Haptoncus luteolus (Erichson) in the field and in the laboratory [19]. Xu et al [81] found that CCRI41 cotton did not affect the population dynamics of non-target pests and predators including ladybirds and spiders in Xinjiang, China.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, ELISA measurements indicated that Bt+CpTI cotton-reared F. virgata and its predator did not contain detectable amounts of the Cry1Ac and CpTI protein. Because the commercial ELISA kit for determining CpTI expression was not available [19] or the amount of CpTI proteins was lower than the lowest limit of quantification [11] , [21] there are few earlier reports on tritrophic interactions involving CpTI protein. On the contrary, many studies related to the transfer of Bt toxic proteins to higher trophic levels have been carried out.…”

Section: Discussionmentioning

confidence: 99%

Effects of Transgenic Cry1Ac + CpTI Cotton on Non-Target Mealybug Pest Ferrisia virgata and Its Predator Cryptolaemus montrouzieri

Zhang

Liu

et al. 2014

PLoS ONE

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Recently, several invasive mealybugs (Hemiptera: Pseudococcidae) have rapidly spread to Asia and have become a serious threat to the production of cotton including transgenic cotton. Thus far, studies have mainly focused on the effects of mealybugs on non-transgenic cotton, without fully considering their effects on transgenic cotton and trophic interactions. Therefore, investigating the potential effects of mealybugs on transgenic cotton and their key natural enemies is vitally important. A first study on the effects of transgenic cotton on a non-target mealybug, Ferrisia virgata (Cockerell) (Hemiptera: Pseudococcidae) was performed by comparing its development, survival and body weight on transgenic cotton leaves expressing Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) with those on its near-isogenic non-transgenic line. Furthermore, the development, survival, body weight, fecundity, adult longevity and feeding preference of the mealybug predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) was assessed when fed F. virgata maintained on transgenic cotton. In order to investigate potential transfer of Cry1Ac and CpTI proteins via the food chain, protein levels in cotton leaves, mealybugs and ladybirds were quantified. Experimental results showed that F. virgata could infest this bivalent transgenic cotton. No significant differences were observed in the physiological parameters of the predator C. montrouzieri offered F. virgata reared on transgenic cotton or its near-isogenic line. Cry1Ac and CpTI proteins were detected in transgenic cotton leaves, but no detectable levels of both proteins were present in the mealybug or its predator when reared on transgenic cotton leaves. Our bioassays indicated that transgenic cotton poses a negligible risk to the predatory coccinellid C. montrouzieri via its prey, the mealybug F. virgata.

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“…However, the fungal stability index was increased with the increasing concentration of root exudates of transgenic cotton, indicating the fungal population and community in the soil was not affected by the much higher concentration of root exudates of transgenic cotton. http 1,2,3,4,5,6,7,8 …”

Section: Figure 4 Effect Of Root Exudates From Transgenic and Nont-tmentioning

confidence: 99%

Soil Ecological Safety Evaluation for Bivalent Transgenic Cotton Plants: Root Exudates Versus Soil Enzyme Activities and Soil Microbial Diversity

Wu¹

2016

Appl Ecol Env Res

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Abstract. Pot experiments were conducted to assess the possibly adverse effect of transgenic Bt-and CpTI-cotton (Bacillus thuringiensis and cowpea trypsin inhibitor cotton) on soil ecosystem. Soil enzymes activities and microbial molecular community and diversity based on 16s-and 18s-PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles were determined by adding root exudates of Bt-and CpTI-cotton into soil. Results showed that the root exudates of transgenic cotton seedlings increased the soil catalase activity by 21.8% to 32.7%. The soil urease activity was decreased by 11.0% while the activity in the treatment of 1ml/350g soil was increased by 18.1% compared to control. The invertase activity in the treatment of 1 ml/350g soil was increased by 54.2%, however, the activity in the treatments of 2 and 4 ml/350 g soil was decreased by from 25% to 29.2%. The total 16sPCR-DGGE lane bands of bacterial community and diversity were almost not different. However, the effect pattern of root exudates of transgenic cotton on soil fungal community and diversity was differed from bacteria. The fungal community number and composition were changed. Though some dominant community disappeared and other newly dominant community appeared, the fungal stability index was increased. Results suggested there was little significantly adverse effect of Bt-and CpTI-cotton on soil ecosystem.

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Pollen from Cry1Ac/CpTI-transgenic cotton does not affect the pollinating beetle Haptoncus luteolus

Cited by 19 publications

References 34 publications

Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Effects of Transgenic Cry1Ac + CpTI Cotton on Non-Target Mealybug Pest Ferrisia virgata and Its Predator Cryptolaemus montrouzieri

Soil Ecological Safety Evaluation for Bivalent Transgenic Cotton Plants: Root Exudates Versus Soil Enzyme Activities and Soil Microbial Diversity

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